Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (14): 2530-2534.doi: 10.3969/j.issn.1673-8225.2011.14.014

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Effect of granulocyte-colony stimulating factor on mobilization and recruitment of very small embryonic-like stem cells

Guo Hui-juan1, Wang Jian1, Zhang Xing-xiu1, Zheng Min2   

  1. 1Department of Neurology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing  400010, China
    2Department of Basic Medical Sciences, Chongqing Medical University, Chongqing  400016, China
  • Received:2010-11-17 Revised:2011-01-23 Online:2011-04-02 Published:2013-11-02
  • Contact: Zheng Min, Associate professor, Master’s supervisor, Department of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China zmdwj625@gmail.com
  • About author:Guo Hui-juan★, Studying for master’s degree, Department of Neurology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China guohuijia88@163.com
  • Supported by:

    the Natural Science Foundation of Chongqing, No. 2010BB5096*; the Science and Technology Subject of Health Bureau of Chongqing, No. 2009-2-207*

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Abstract:

BACKGROUND: A population of very small embryonic-like stem cells (VSEL-SCs) is a kind of non-hemopoietic stem cells, which has similar biological characteristics to embryonic stem cells. Currently, most studies concerning VSEL-SCs after myocardial infarction, but mobilization of VSEL-SCs and its effect on repair damaged tissues after acute cerebral infarction remain poorly understood. 
OBJECTIVE: To investigate the effects of granulocyte-colony stimulating factor (G-CSF) on mobilization of bone marrow-derived VSEL-SCs, recruitment and mechanism after mouse acute cerebral infarction.
METHODS: Mouse middle cerebral artery occlusion (MCAO) model was induced by using the filament occlusion method. G-CSF and normal saline were separately injected to MCAO model. Neurological scales were evaluated. The number of VSEL-SCs mobilized into peripheral blood was checked by fluorescence-activated cell sorting analysis. The level of stromal cell derived factor-1 (SDF-1) in plasma and cerebral tissue were determined by ELISA assay. Positive expression of SDF-1 in ischemic regions was detected by immunohistochemistry.
RESULTS AND CONCLUSION: Compared with the normal saline group, the neurological behavioral scale of G-CSF treated group was significantly lower at 108 hours after operation (P  < 0.05). G-CSF groups mobilized more VSEL-SCs into peripheral blood compared with the operated control group at 72 and 108 hours (P  < 0.05). The SDF-1 density significantly increased in the plasma and brain tissues of the G-CSF group than that of the normal saline group (P < 0.05), and positive correlation can be seen between the number of VSEL-SCs mobilized into peripheral blood and the SDF-1 plasma concentration. The positive expression of SDF-1 in the G-CSF group was greater than that of the normal saline group. The results indicated that G-CSF mobilized VSEL-SCs in adult MCAO mouse bone marrow into peripheral blood. The mechanism of beneficial effects of G-CSF possibly related to increase of SDF-1 expression in the infracted tissues and recruitment of more VSEL-SCs through CXCR4/SDF-1 axis.

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