Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (14): 2535-2539.doi: 10.3969/j.issn.1673-8225.2011.14.015

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A natural activity factor promotes cardiogenic differentiation of bone marrow derived mesenchymal stem cells

Peng Peng1, Mo Ye-hua1, Lin Xiao-li2, Li Zhong-yu2, Cheng Lei2, Zhou Zhen2, Li Ming1,2   

  1. 1Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou  510632, Guangdong Province, China
    2Laboratory of Innovative Medicine, Hong Kong Special Administrative Region, China
  • Received:2010-12-14 Revised:2011-01-28 Online:2011-04-02 Published:2013-11-02
  • Contact: Li Ming, Doctor, Professor, Doctoral supervisor, Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, Guangdong Province, China; Laboratory of Innovative Medicine, Hong Kong Special Administrative Region, China li.ming@labinnovativemedicine.com
  • About author:Peng Peng★, Studying for master’s degree, Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, Guangdong Province, China xuanxuan86120@163.com
  • Supported by:

    the RGC grant (2140569) from Hong Kong Government Research Committee No. 470707*

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Abstract:

BACKGROUND: Previous studies have demonstrated that the methanol extract of Geum japonicum (EGJ) can effectively promote the cardiogenic differentiation of the exogenous mesenchymal stem cells (MSCs), leading to substantial repair of infarcted heart with cardiac function significantly improved. In this study, the isolation of the active compound, which can induce cardiogenic differentiation of MSCs in cell culture system, was described through a bioassay guided fractionation strategy.
OBJECTIVE: To isolate active compound (cardiogenin) from Geum japonicum and to observe its activity in promoting cardiogenic differentiation of MSCs.
METHODS: The MSCs were isolated from the bone marrow of SD rats. The isolated MSCs were cultured and treated with EGJ (100 mg/L) and cardiogenin (10 mg/L) respectively. Immunofluorescent staining with antibodies specific for early cardiac differentiation marker (MEF2) and myosin heavy chain (MHC) was performed at 3 and 7 days after treatment respectively to monitor the process of cardiogenic differentiation of MSCs.
RESULTS AND CONCLUSION: It was found that approximately 39% of both EGJ and cardiogenin treated MSCs were positively stained with MEF2 at 3 days after treatment. On day seven of the treatment, more than 70% of both EGJ and cardiogenin treated MSCs were positively stained with MHC specific antibodies. The cardiogenin can significantly induce cardiogenic differentiation of MSCs in vitro as does EGJ, but the dose of cardiogenin required for induction of cardiopgenic differentiation of MSCs is much less but the induction efficiency is little higher than that of EGJ. 

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