Chinese Journal of Tissue Engineering Research ›› 2025, Vol. 29 ›› Issue (1): 65-73.doi: 10.12307/2024.736
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Wang Zhikun1, Bai Shaoxuan2, Zhao Wei2, Wang Chenyu1
Received:
2023-10-26
Accepted:
2023-11-25
Online:
2025-01-08
Published:
2024-05-18
Contact:
Wang Chenyu, MD, Associate professor, Zhengzhou University of Aeronautics, Zhengzhou 450015, Henan Province, China
About author:
Wang Zhikun, Master, Lecturer, Zhengzhou University of Aeronautics, Zhengzhou 450015, Henan Province, China
Supported by:
CLC Number:
Wang Zhikun, Bai Shaoxuan, Zhao Wei, Wang Chenyu. Exercise preconditioning combined with bone marrow mesenchymal stem cell transplantation for myocardial infarction in rats[J]. Chinese Journal of Tissue Engineering Research, 2025, 29(1): 65-73.
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2.1 骨髓间充质干细胞鉴定结果 通过流式细胞仪测定细胞表面抗原阳性率,结果发现,CD34阳性率为1.74%(< 5%),CD90和CD44阳性率分别为98.1%和97.5%(> 95%),所检测细胞表面标志物符合大鼠骨髓间充质干细胞表面抗原表达类型,见图2。 2.2 各组大鼠运动能力比较 实验前,各组运动能力(峰值跑速、力竭距离、力竭时间)比较均无显著性差异(P > 0.05)。与实验前比较,实验后模型组、移植组和联合组运动能力均下降(P < 0.05),假手术组无显著性差异(P > 0.05);与假手术组比较,模型组运动能力降低(P < 0.05);与模型组比较,移植组和联合组运动能力升高(P < 0.05);与移植组比较,联合组运动能力各参数进一步提升(P < 0.05),见表2。 "
2.3 各组大鼠心脏结构、功能及左心室血液动力学比较 各组心脏结构和功能见表3。与假手术组比较,模型组心脏质量、心脏质量指数、左心室质量、左心室质量指数、心肌梗死面积、左心室舒张末期内径增加(P < 0.05),左心室射血分数降低(P < 0.05);与模型组比较,移植组心脏质量、心脏质量指数、左心室质量、左心室质量指数、心肌梗死面积、左心室舒张末期内径下降(P < 0.05),左心室射血分数升高(P < 0.05),联合组心肌梗死面积降低(P < 0.05),左心室射血分数升高(P < 0.05),心脏质量、心脏质量指数、左心室质量、左心室质量指数、左心室舒张末期内径无显著性变化(P > 0.05);与移植组比较,联合组心脏质量、心脏质量指数、左心室质量、左心室质量指数、左心室舒张末期内径、左心室射血分数升高(P < 0.05),心肌梗死面积降低(P < 0.05)。"
2.4 各组大鼠心肌组织病理学观察结果 心肌纵切面苏木精-伊红染色显示:假手术组心肌纤维排列整齐,胞浆染色均匀,细胞核边界清晰;模型组心肌纤维排列紊乱,可见大量中性粒细胞浸润,广泛坏死;与模型组比较,移植组和联合组上述组织病理学改变均明显减轻,见图3A。 心肌横切面苏木精-伊红染色显示:与假手术组比较,模型组心肌细胞肿大,心肌细胞横截面积较明显增加(P < 0.05);与模型组比较,移植组心肌细胞肿大明显减轻,心肌细胞横截面积显著下降(P < 0.05);与移植组比较,联合组心肌细胞横截面积升高(P < 0.05),见图3B和图3C。 心肌Masson染色显示:假手术组几乎无胶原纤维;模型组细胞间质存在大量胶原纤维并发生心肌纤维化,胶原容积分数较假手术组明显升高(P < 0.05);移植组和联合组胶原沉积明显减轻,胶原容积分数较模型组明显下降(P < 0.05);与移植组比较,联合组胶原容积分数较模型组进一步降低(P < 0.05),见图3D,E。 2.5 各组大鼠心肌胚胎基因mRNA表达量比较 与假手术组比较,模型组心房钠尿肽、脑钠肽、β-肌球蛋白重链mRNA表达量上调(P < 0.05);与模型组比较,移植组和联合组上述各胚胎基因mRNA表达量均下调(P < 0.05);与移植组比较,联合组各胚胎基因mRNA表达量进一步下降(P < 0.05),见图4。"
2.6 各组大鼠心肌炎症因子mRNA表达量比较 与假手术组比较,模型组白细胞介素1β、白细胞介素6、肿瘤坏死因子α mRNA表达量上调(P < 0.05),白细胞介素10 mRNA表达量下调(P < 0.05);与模型组比较,移植组白细胞介素1β、白细胞介素6、肿瘤坏死因子α(除移植后4周)mRNA表达量下调(P < 0.05),白细胞介素10 mRNA表达量无显著性变化(P > 0.05),联合组各促炎症因子表达量下调(P < 0.05),白细胞介素10 mRNA表达量上调(P < 0.05);与移植组比较,联合组各促炎症因子mRNA表达量下降(P < 0.05),白细胞介素10 mRNA表达量升高(P < 0.05),见图5。 "
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