Chinese Journal of Tissue Engineering Research ›› 2024, Vol. 28 ›› Issue (16): 2568-2573.doi: 10.12307/2024.306
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Han Weina1, Xu Xiaoqing1, Shi Jinning1, Li Xinru2, Cai Hongyan3
Received:
2023-03-22
Accepted:
2023-04-20
Online:
2024-06-08
Published:
2023-07-31
Contact:
Han Weina, Department of Physiology, Puai School of Medicine (Health Science Center), Shaoyang University, Shaoyang 422000, Hunan Province, China
About author:
Han Weina, Master, Associate professor, Department of Physiology, Puai School of Medicine (Health Science Center), Shaoyang University, Shaoyang 422000, Hunan Province, China
Supported by:
CLC Number:
Han Weina, Xu Xiaoqing, Shi Jinning, Li Xinru, Cai Hongyan. Prediction and validation of potential targets for the glucagon-like peptide-1 receptor agonist in the treatment of Alzheimer’s disease[J]. Chinese Journal of Tissue Engineering Research, 2024, 28(16): 2568-2573.
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2.4 利拉鲁肽可以抑制Aβ对HT22细胞的损伤作用,且100 nmol/L为最佳浓度 实验采用CCK-8试剂盒检测了培养细胞的活性,确定100 nmol/L利拉鲁肽是拮抗Aβ1-42的最佳浓度,见图4。0 nmol/L利拉鲁肽组与对照组相比细胞活性明显下降,且有显著性差异(P < 0.001),提示Aβ有细胞毒性作用;40,80,100,150,200 nmol/L浓度的利拉鲁肽均可以拮抗Aβ的细胞毒性作用,其中100 nmol/L时细胞活性最佳,且与0 nmol/L组相比有显著性差异(P < 0.01)。由于150 nmol/L浓度的利拉鲁肽组细胞活力开始下降,因此提示100 nmol/L浓度的利拉鲁肽为拮抗Aβ1-42的最佳干预剂量。"
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