Chinese Journal of Tissue Engineering Research ›› 2024, Vol. 28 ›› Issue (3): 329-335.doi: 10.12307/2023.985

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Effects of enamel adhesives with different components on Porphyromonas gingivalis and Streptococcus mutans

Li Zhiyao1, Hu Zheng2, Li Xuan3, 4, Lu Peijun2   

  1. 1Department of Oral Surgery, Shanghai Xuhui District Dental Center, Shanghai 200032, China; 2Department of Orthodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology, Shanghai 200011, China; 3Department of Orthodontics, Shanghai Stomatological Hospital, Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Shanghai 200001, China; 4Department of Stomatology, Children’s Hospital of Fudan University, Shanghai 201103, China
  • Received:2023-01-16 Revised:2023-03-02 Online:2024-01-28 Published:2023-07-06
  • Contact: Lu Peijun, Master, Attending physician, Department of Orthodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology, Shanghai 200011, China
  • About author:Li Zhiyao, Master, Attending physician, Department of Oral Surgery, Shanghai Xuhui District Dental Center, Shanghai 200032, China Hu Zheng, MD, Attending physician, Department of Orthodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology, Shanghai 200011, China
  • Supported by:
    Youth Fund Project of Shanghai Municipal Health Bureau, No. 20144Y0219 (to LX)

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Abstract: BACKGROUND: The light curing and fluoride light curing enamel adhesives have a certain sealing effect on the etched enamel surface. The fluoride light curing enamel adhesives can also achieve the anti-caries function by releasing fluoride ions. However, the existing researches lack the long-term tracing of fluoride release effect, especially the amount of local pathogenic bacteria after 1-3 months of local fluoride application.
OBJECTIVE: To analyze the changes in the expression of Porphyromonas gingivalis and Streptococcus mutans in subgingival plaque of the upper anterior teeth adhered by different components of enamel adhesives in adolescent patients with fixed appliance.
METHODS: Ninety adolescent patients who received orthodontic treatment in Shanghai Stomatological Hospital from January to December 2016 were enrolled, including 43 males and 47 females, with a mean age of (13.27±1.12) years. These patients were randomly divided into three groups (n=30 per group). In the chemical curing group, Unite™ bonding resin was used to bond fixed appliances. In the light curing group, Transbond XT light curing resin was used to bond the fixed appliance. In the fluoride light curing group, GC light curing orthodontic adhesive was used to bond the fixed appliance with cement. The subgingival plaque was collected on the day of bonding, 1st, 2nd, and 3rd month follow-up reviews. The expressions of Porphyromonas gingivalis and Streptococcus mutans in subgingival plaque were detected by PCR.
RESULTS AND CONCLUSION: (1) Intragroup comparison: With the increase of bonding time, Porphyromonas gingivalis expression increased significantly in the 3rd month in the chemical curing group (P < 0.05). In the light curing group, Porphyromonas gingivalis showed a significant decrease in the 1st month (P < 0.05). Porphyromonas gingivalis expression decreased significantly in the 1st and 2nd months compared with initial data in the fluoride light curing group (P < 0.05). The expression of Streptococcus mutans was higher in the chemical curing group in the 1st, 2nd, and 3rd months compared with the initial data (P < 0.05). In the fluoride light curing group, the expression of Streptococcus mutans was lower in the 1st, 2nd, and 3rd months compared with the initial data (P < 0.05). There was no significant difference in the proliferation and expression of Streptococcus mutans during follow-up in the light curing group compared to the initial adhesion (P > 0.05). (2) Intergroup comparison: In the 1st month, the expression of Porphyromonas gingivalis was lower in the light curing group and fluoride light curing group than that in the chemical curing group (P < 0.05). In the 2nd and 3rd months, the expression of Porphyromonas gingivalis was lower in the fluoride light curing group than that in the light curing group and chemical curing group (P < 0.05). In the 1st, 2nd, and 3rd months, the expression of Streptococcus mutans was lower in the light curing group and fluoride light curing group than that in the chemical curing group (P < 0.05). The expression of Streptococcus mutans was lower in the fluoride light curing group than that in the light curing group (P < 0.05). (3) The results show that in the fixed orthodontic process, the use of different components of enamel adhesives has different effects on the proliferation and expression of oral Porphyromonas gingivalis and Streptococcus mutans in the short term. Fluoride light curing enamel adhesives at the initial stage can reduce the occurrence of enamel demineralization, caries, and periodontal inflammation. 

Key words: orthodontics, fixed appliance, enamel adhesives, Porphyromonas gingivalis, Streptococcus mutans

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