Depression-like behavior characteristics of rats with neuropathic pain and expression of mGluR5 and NMDAR2B in the left dorsal agranular insular area

doi:10.12307/2023.410

Abstract

Abstract: BACKGROUND: The occurrence of neuropathic pain and the resulting depression may be related to the increased protein expression of metabotropic glutamate receptor 5 (mGluR5) and N-methyl-D-aspartate receptor type 2B (NMDAR2B) in the brain.
OBJECTIVE: To explore the behavioral characteristics of pain and depression in rats with neuropathic pain caused by chronic compressive injury of the sciatic nerve and to observe the expression of mGluR5 and NMDAR2B proteins in the left dorsal agranular insular area of the rats with neuropathic pain.
METHODS: Fifty Sprague-Dawley rats were divided into sham operation group (n=25; only the sciatic nerve trunk was exposed without ligation) and model group (n=25; the sciatic nerve was ligated to establish a chronic compressive injury model). Von Frey filaments were used to detect mechanical paw withdrawal threshold in rats. Depression-like behaviors were observed by the sugar water preference test and forced swimming test. The sciatic nerve function index and hematoxylin-eosin staining were used to observe the function and structure of the sciatic nerve. The metabolism of the sciatic nerve and brain was observed by [18]F-FDG PET-CT. The expression levels of mGluR5 and NMDAR2B in the left-brain dorsal agranular insular area were detected by western blot.
RESULTS AND CONCLUSION: Compared with the sham operation group, the mechanical paw withdrawal threshold and sciatic nerve function index were significantly decreased in the model group at 1 week after operation (P < 0.05), and depression-like behaviors appeared and progressively worsened in 2-3 weeks (P < 0.05). Hematoxylin-eosin staining results showed that the pathological changes of the sciatic nerve in the model group were progressively aggravated. The average standardized intake value of the right sciatic nerve/liver in the model group was significantly higher than that in the sham operation group in 2-4 weeks after operation (P < 0.05). The expression levels of mGluR5 and NMDAR2B in the left dorsal agranular insular area were significantly higher in the model group than the sham operation group (P < 0.05). To conclude, the pathological manifestations, sciatic nerve function, neuropathic pain, and depression-like behaviors are not synchronized after sciatic nerve injury. mGluR5 and NMDAR2B in the left dorsal agranular insular area may be involved in the pathogenesis of neuropathic pain and depression-like behaviors caused by pain.

Key words: neuropathic pain, chronic compression injury of the sciatic nerve, dorsal agranular insular area, mGluR5, NMDAR2B, rat, depression-like behavior

CLC Number:

Hu Yue, Zhu Yuanliang, Wan Tenggang, Xu Fangyuan, Xu Zhangyu, Li Jiyang, Li Dan, Wang Jianxiong. Depression-like behavior characteristics of rats with neuropathic pain and expression of mGluR5 and NMDAR2B in the left dorsal agranular insular area[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(20): 3216-3223.

Figures/Tables 8

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