Chinese Journal of Tissue Engineering Research ›› 2022, Vol. 26 ›› Issue (17): 2713-2719.doi: 10.12307/2022.540
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Han Xuke1, 2, Chen Yiding1, 2, Chen Huizhen1, 2, Yang Xiaomei1. 2, Hong Peipei1, 2, Chen Qiu1, 2
Received:
2021-03-29
Revised:
2021-05-07
Accepted:
2021-09-27
Online:
2022-06-18
Published:
2021-12-24
Contact:
Chen Qiu, Professor, Doctoral supervisor, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, Sichuan Province, China; Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan Province, China
About author:
Han Xuke, MD candidate, Physician, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, Sichuan Province, China; Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan Province, China
Supported by:
CLC Number:
Han Xuke, Chen Yiding, Chen Huizhen, Yang Xiaomei, Hong Peipei, Chen Qiu. Establishment and evaluation of neurogenic bladder model in rats with type 2 diabetes mellitus[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(17): 2713-2719.
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2.1 实验动物数量分析 选取雄性SD大鼠55只,分为4组,其中正常组10只,在整个实验过程中无脱失;链脲佐菌素20,35,50 mg/kg分别15只,用于2型糖尿病建模。其中链脲佐菌素20,35 mg/kg组在造模过程中分别死亡3只,链脲佐菌素50 mg/kg组在造模过程中死亡5只,剩余34只全部纳入实验结果进行分析。 2.2 不同剂量链脲佐菌素对成模率和存活率的影响 3个模型组入组各15只大鼠,在第4周链脲佐菌素注射后,链脲佐菌素20 mg/kg组分别于第5,6,7周死亡大鼠1只,至第8周检测时存活12只;链脲佐菌素35 mg/kg组于第5周死亡大鼠2只,第7周死亡1只,至第8周检测时存活12只;链脲佐菌素50 mg/kg组于第5周死亡大鼠3只,第6,7周各死亡1只,至第8周检测时存活10只。相较于链脲佐菌素35 mg/kg组(中间剂量),链脲佐菌素50 mg/kg组(高剂量)大鼠存活率较低(P < 0.05)。而成模率方面,相较于链脲佐菌素35 mg/kg组,链脲佐菌素20 mg/kg组(低剂量)大鼠的成模率较低(P < 0.05),见表1。通过统计分析发现,链脲佐菌素35 mg/kg组成模率和存活率均较高,稳定性较好。"
2.3 一般情况 与正常组相比,模型组大鼠建模前初始体质量无明显差异。正常组大鼠体质量增加,血糖平稳;模型组大鼠在链脲佐菌素注射3 d后,出现多饮、多尿、多食的典型糖尿病症状。第4 周时模型组大鼠精神萎靡,活动度降低,体质量明显升高(P < 0.01),空腹血糖升高(P < 0.01);与正常组大鼠相比,第8周时模型组大鼠皮毛光泽度晦暗,活动度及精神状态明显变差,体质量下降(P < 0.05),空腹血糖明显升高(P < 0.01),均≥16.7 mmol/L。链脲佐菌素20,35,50 mg/kg组间体质量相比,差异无显著性意义(P > 0.05);但在第4周时,链脲佐菌素50 mg/kg组的血糖明显高于另外两组(P < 0.05);第8周时,链脲佐菌素35 mg/kg组血糖水平上升,与链脲佐菌素50 mg/kg组相比差异无显著性意义(P > 0.05),仅链脲佐菌素20 mg/kg组血糖水平明显低于链脲佐菌素50 mg/kg组(P < 0.05),见表2。"
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