Chinese Journal of Tissue Engineering Research ›› 2022, Vol. 26 ›› Issue (19): 3024-3029.doi: 10.12307/2022.381

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Effect of bone marrow mesenchymal stem cell conditioned medium on the proliferation of multiple myeloma cells

Huang Mei1, Wang Feiqing2, Liang Huiling2, Yang Xu2, Zhao Jianing2, Wang Kun2, Liu Yanqing2, Zhou Yuan2, Wang Jishi1, 3, Li Yanju1, 3, Liu Yang2, 4   

  1. 1Guizhou Medical University, Guiyang 550004, Guizhou Province, China; 2First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 550001, Guizhou Province, China; 3Department of Hematology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China; 4National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique, Guizhou Medical University, Guiyang 550004, Guizhou Province, China
  • Received:2021-06-01 Revised:2021-07-24 Accepted:2021-09-01 Online:2022-07-08 Published:2021-12-28
  • Contact: Li Yanju, MD, Associate chief physician, Guizhou Medical University, Guiyang 550004, Guizhou Province, China; Department of Hematology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China Liu Yang, MD, Associate professor, First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 550001, Guizhou Province, China; National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique, Guizhou Medical University, Guiyang 550004, Guizhou Province, China
  • About author:Huang Mei, Master candidate, Guizhou Medical University, Guiyang 550004, Guizhou Province, China
  • Supported by:
    the National Natural Science Foundation of China, No. 31660326 (to LY); China Postdoctoral Science Foundation, No. 2018M640938 (to LY), No. 43XB3794XB (to LYJ); the grant from the Science and Technology Department of Guizhou Province, No. [2016]1019 (to LY), No. LH[2016]7505 (to LYQ), No. LH[2017]7140 (to WFQ); the Top Talent Program of Guizhou Provincial Department of Education, No. KY[2016]074 (to LY); the Project Foundation of Guizhou Administration of Traditional Chinese Medicine, No. QZYY-2018-062 (to ZY); the grant from the Guizhou University of Traditional Chinese Medicine, No. [2019]45 (to WK)

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Abstract: BACKGROUND: In recent years, relationship between the bone marrow microenvironment and the growth, survival and drug resistance of multiple myeloma cells has become a research hotspot for researchers. Few studies have evaluated the effects of bone marrow mesenchymal stem cells conditioned culture medium on multiple myeloma cell lines.  
OBJECTIVE: To investigate the effect of bone marrow mesenchymal stem cell conditioned medium on the proliferation of multiple myeloma cell lines.
METHODS:  Ficoll density gradient centrifugation and adherent method were used to purify bone marrow mesenchymal stem cells from healthy donors. The supernatants of 3-5 generations of bone marrow mesenchymal stem cells were collected in the culture medium. Ultrafiltration centrifuge tube was used to prepare bone marrow mesenchymal stem cell conditioned medium and to prepare 1640 complete medium containing 10% human bone marrow mesenchymal stem cell conditioned medium so as to induce the culture of multiple myeloma cell lines RPMI8226 and U266 for 48 hours. Cell proliferation was detected by MTT assay and EdU fluorescence staining. Cell apoptosis was detected by flow cytometry and PI fluorescence staining. Real-time PCR and western-blot assay were used to detect mRNA and protein levels of multiple myeloma cell lines BCL-2 and BTK.  
RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cell conditioned medium could promote the proliferation of multiple myeloma cell lines (P < 0.05), reduce the apoptosis rate of cancer cells (P < 0.05) and was associated with high mRNA and protein expression of BCL-2 and BTK (P < 0.05). The results showed that the bone marrow mesenchymal stem cell conditioned medium promoted the proliferation multiple myeloma cell lines.

Key words: ">bone marrow mesenchymal stem cells, multiple myeloma, conditioned medium, proliferation, apoptosis

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