Wnt/beta-catenin signaling pathway in the treatment of chronic obstructive pulmonary disease with bone marrow mesenchymal stem cells

doi:10.12307/2022.321

Abstract

Abstract: BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) have a wide application prospect for the treatment of respiratory diseases. In recent years, studies have shown that the Wnt/β-catenin signaling pathway plays an important role in regulating the directional differentiation of BMSCs and promoting paracrine function.
OBJECTIVE: To investigate the effect of Wnt/β-catenin signaling pathway activation on chronic obstructive pulmonary disease after transplantation with BMSCs.
METHODS: Whole bone marrow adherence method was used to extract rat BMSCs. Rat BMSCs were infected with Wnt3a lentivirus. The chronic obstructive pulmonary disease rat model was constructed by intratracheal infusion of lipopolysaccharide combined with smoking method. After model establishment, BMSCs, GFP-BMSCs, and Wnt3a-BMSCs were implanted through the tail vein. PBS was injected through the tail vein in the model and normal control groups. The rats in each group were sacrificed at 7, 14, and 28 days. The pathological changes of rat lung tissue were observed by hematoxylin-eosin staining. The levels of interleukin-10 and tumor necrosis factor alpha in arterial blood were detected by ELISA.
RESULTS AND CONCLUSION: (1) The pathological results of hematoxylin-eosin staining showed that the pathological changes of the three BMSCs transplantation groups were significantly improved compared with the model group, and the Wnt3a-BMSCs group improved significantly. (2) At the same time point, the arterial blood tumor necrosis factor alpha level was lower in the three BMSCs transplantation groups than that in the model group (all P < 0.05), and there was little difference between the BMSCs group and the GFP-BMSCs group. However, the level in the Wnt3a-BMSCs group was relatively lower than that in the other two groups (P < 0.05). Arterial blood interleukin-10 level was higher in the three BMSCs transplantation groups than that in the model group (all P < 0.05), among which there was little difference between the BMSCs group and the GFP-BMSCs group. However, the level in the Wnt3a-BMSCs group was significantly higher than that in the other two groups (P < 0.05). (3) The results show that transplantation of BMSCs overexpressing Wnt3a (after Wnt/β-catenin signaling pathway activation) into chronic obstructive pulmonary disease rats can significantly improve the pathological changes of lung tissue, increase the expression of anti-inflammatory factor interleukin-10 and reduce the expression of tumor necrosis factor alpha, and enhance the anti-inflammatory effect of BMSCs.

Key words: stem cells, bone marrow mesenchymal stem cells, chronic obstructive pulmonary disease, Wnt3a, Wnt/?-catenin signaling pathway, interleukin-10, tumor necrosis factor alpha

CLC Number:

Zhong Yulan, Zhou Xiangxiang, Gan Xin. Wnt/beta-catenin signaling pathway in the treatment of chronic obstructive pulmonary disease with bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(13): 1979-1984.

Figures/Tables 9

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