Mechanism of Ermiao San in the treatment of rheumatoid arthritis

doi:10.12307/2022.121

Abstract

Abstract: BACKGROUND: Ermiao San has a good effect in the treatment of rheumatoid arthritis. Studies have proved that Ermiao San can inhibit angiogenesis and delay the progression of the disease, but its mechanism is not clear.
OBJECTIVE: To analyze the pharmacological mechanism of Ermiao San in the treatment of rheumatoid arthritis based on network pharmacology and molecular docking, so as to provide reference for studying the therapeutic mechanism and clinical application of classical prescriptions.
METHODS: The main chemical components and targets of Atractylodes macrocephala and Phellodendri were collected on the Systematic Pharmacological Platform of Traditional Chinese Medicine (TCMSP), and the active components of traditional Chinese medicine were screened according to ADME. The main targets of rheumatoid arthritis were obtained by GeneCards, OMIM, TTD and DRUGBANK databases, and the protein interaction was analyzed by String platform to construct the protein-protein interaction network and mine the potential protein functional modules in the network. The “drug-ingredient-target” and the relevant biological processes and signaling pathways were analyzed by Metascape platform, and then the “Ermiao San component-rheumatoid arthritis target-pathway” network was constructed by Cytoscape3.8.0 software. Finally, the molecular docking verification was carried out between the drug components with high value in the network and the core action target by Autodock software.
RESULTS AND CONCLUSION: The core active components of Ermiao San in the treatment of rheumatoid arthritis are quercetin, wogonin, β-sitosterol and so on, and the core targets are prostaglandin G/H synthetase 2, JUN proto-oncogene protein, and nuclear factor-κb/p65. The biological pathways of Ermiao San in the treatment of rheumatoid arthritis mainly include advanced glycation end product and receptor, mitogen-activated protein kinase, interleukin-17, and tumor necrosis factor signaling pathway, which are mainly related to immune regulation, apoptosis, inflammatory reaction and oxidative stress. The results of molecular docking showed that the key compounds such as quercetin, wogonin and β-sitosterol in Ermiao San have stable binding activity with key targets such as prostaglandin G/H synthetase 2, JUN proto-oncogene protein, and nuclear factor-κb/p65. This study preliminarily indicates that Ermiao San plays a role in the treatment of rheumatoid arthritis through multi-component, multi-target and multi-pathway regulations, which provides a basis for the study on the mechanism and clinical application of Ermiao San.

Key words: Ermiao San, traditional Chinese medicine, network pharmacology, molecular docking, rheumatoid arthritis, target prediction, immune regulation, inflammatory response

CLC Number:

Zhao Yuwei, Gao Yuting, Li Zhen, Hao Huiqin . Mechanism of Ermiao San in the treatment of rheumatoid arthritis[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(5): 742-748.

Figures/Tables 13

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