Chinese Journal of Tissue Engineering Research ›› 2022, Vol. 26 ›› Issue (2): 245-252.doi: 10.12307/2022.040
Previous Articles Next Articles
Zhang Xiaoyun1, 2, Li Huanan1, Chen Feng2, Chai Yuan2, Gan Bin1, Li Song1, Chen Dingpeng1
Received:
2020-09-27
Revised:
2020-09-30
Accepted:
2020-10-24
Online:
2022-01-18
Published:
2021-10-27
Contact:
Li Huanan, MD, Doctoral supervisor, Chief physician, School of Clinical Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, Jiangxi Province, China
About author:
Zhang Xiaoyun, MD candidate, Associate chief physician, School of Clinical Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, Jiangxi Province, China; Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530011, Guangxi Zhuang Autonomous Region, China
Supported by:
CLC Number:
Zhang Xiaoyun, Li Huanan, Chen Feng, Chai Yuan, Gan Bin, Li Song, Chen Dingpeng. Potential molecular mechanism of Guizhi Shaoyao Zhimu Decoction in the treatment of gouty arthritis based on network pharmacology and molecular docking[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(2): 245-252.
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks
2.5 GO富集分析和KEGG富集分析结果 利用DAVID数据库对潜在靶点进行GO及KEGG功能富集分析,见图4。GO富集分析共确定了846个与痛风性关节炎发生发展过程密切相关的条目,其中805条生物学过程的相关条目主要包括对脂多糖的反应、对细菌来源分子的反应、细胞对生物刺激的反应、氧化应激反应、炎症反应的调节和对活性氧的反应等;9个细胞成分的相关条目主要包括膜筏、微域膜和膜区等;共32个分子功能的相关条目主要包括血红素结合、四吡咯结合、丝氨酸型内肽酶活性、丝氨酸型肽酶活性、磷酸酶结合、丝氨酸水解酶活性、铵离子结合、MAP激酶活性和激活转录因子结合等。KEGG通路结果表明,桂枝芍药知母汤治疗痛风性关节炎可能的作用机制通路主要涉及白细胞介素17通路,肿瘤坏死因子通路、AGE-RAGE通路、NOD样受体通路、核转录因子κB通路和Toll样受体通路等,其中白细胞介素17通路图见图5。"
[1] TATSIY O, MAYER TZ, CARALHO OLIVEIRA V, et al. Cytokine production and NET formation by monosodium urate-activated human neutrophils involves early and late events, and requires upstream TAK1 and Syk. Front Immunol. 2020. doi: 10.3389/fimmu.2019.02996. [2] HSIEH CY, LI LH, LAM Y, et al. Synthetic 4-hydroxy auxarconjugatin b, a novel autophagy inducer, attenuates gouty inflammation by inhibiting the NLRP3 inflammasome. Cells. 2020;9(2):279. [3] 舒建龙,李凤珍,覃裕旺.痛风病理及临床中西医治疗的研究进展[J].中国实验方剂学杂志, 2020,26(2):218-227. [4] 黄 聪,彭伟,牟茂婷,等.基于“肢节痹病”的桂枝芍药知母汤的现代药理作用探讨[J].时珍国医国药,2019,30(4):949-950. [5] 乔晓涵.桂枝芍药知母汤加减方治疗急性痛风性关节炎疗效观察[D].沈阳:辽宁中医药大学, 2019. [6] LI G, XU Q, HAN K, et al. Experimental evidence and network pharmacology-based analysis reveal the molecular mechanism of Tongxinluo capsule administered in coronary heart diseases. Biosci Rep. 2020;40(10):BSR20201349. [7] SAKLE NS, MORE SA, MOKALE SN. A network pharmacology-based approach to explore potential targets of Caesalpinia pulcherima: an updated prototype in drug discovery. Sci Rep. 2020;10(1):17217. [8] ZHANG Q, LI R, PENG W, et al. Identification of the active constituents and significant pathways of Guizhi-Shaoyao-Zhimu decoction for the treatment of diabetes mellitus based on molecular docking and network pharmacology. Comb Chem High Throughput Screen. 2019; 22(9):584-598. [9] 汪选斌,陆金健.中药药理规范研究的思考[J].世界科学技术-中医药现代化,2019,21(9):1846-1854. [10] GUO Q, MAO X, ZHANG Y, et al. Guizhi-Shaoyao-Zhimu decoction attenuates rheumatoid arthritis partially by reversing inflammation-immune system imbalance. J Transl Med. 2016;14(1):165. [11] ZHANG Q, PENG W, WEI S, et al. Guizhi-Shaoyao-Zhimu decoction possesses anti-arthritic effects on type II collagen-induced arthritis in rats via suppression of inflammatory reactions, inhibition of invasion & migration and induction of apoptosis in synovial fibroblasts. Biomed Pharmacother. 2019;118:109367. [12] ZHANG KH, WANG MQ, WEI LL, et al. Investigation of the effects and mechanisms of dendrobium loddigesii rolfe extract on the treatment of gout. Evid Based Complement Alternat Med. 2020; 2020:4367347. [13] ZHOU Q, SUN HJ, LIU SM, et al. Anti-inflammation effects of the total saponin fraction from Dioscorea nipponica Makino on rats with gouty arthritis by influencing MAPK signalling pathway. BMC Complement Med Ther. 2020;20(1):261. [14] ZHOU M, ZE K, HUA L, et al. Cyr61 promotes inflammation of a gouty arthritis model in rats. Mediators Inflamm. 2020;2020:8298615. [15] 李建,张洁瑛,孙鹏,等.平胃散合桂枝芍药知母汤加减治疗慢性痛风性关节炎的疗效机制[J].中国实验方剂学杂志,2018,24(1):180-185. [16] RUIZ-MIYAZAWA KW, STAURENGO-FERRARI L, MIZOKAMI SS, et al. Quercetin inhibits gout arthritis in mice: induction of an opioid-dependent regulation of inflammasome. Inflammopharmacology. 2017;25(5):555-570. [17] FU X, QIN T, YU J, et al. Formononetin ameliorates cognitive disorder via pgc-1α pathway in neuroinflammation conditions in high-fat diet-induced mice. CNS Neurol Disord Drug Targets. 2019;18(7):566-577. [18] WANG S, CAO M, XU S, et al. Luteolin alters macrophage polarization to inhibit inflammation. Inflammation. 2020;43(1):95-108. [19] LI P, LI Y, JIANG H, et al. Glabridin, an isoflavan from licorice root, ameliorates imiquimod-induced psoriasis-like inflammation of BALB/c mice.Int Immunopharmacol. 2018;59:243-251. [20] 李想,李冀.甘草提取物活性成分药理作用研究进展[J].江苏中医药,2019,51(5):81-86. [21] Wu SC, YANG ZQ, LIU F, et al. Antibacterial effect and mode of action of flavonoids from licorice against methicillin-resistant staphylococcus aureus. Front Microbiol. 2019;10:2489. [22] YUAN X, LI N, ZHANG M, et al. Taxifolin attenuates IMQ-induced murine psoriasis-like dermatitis by regulating T helper cell responses via Notch1 and JAK2/STAT3 signal pathways. Biomed Pharmacother. 2020;123:109747. [23] HAUGEN J, CHANDYO RK, BROKSTAD KA, et al. Cytokine concentrations in plasma from children with severe and non-severe community acquired pneumonia. PLoS One. 2015;10(9):e0138978. [24] PISANI LP, ESTADELLA D, RIBEIRO DA. The role of toll like receptors (TLRs) in oral carcinogenesis. Anticancer Res. 2017;37(10):5389-5394. [25] CHEN Y, WU D, SUN L. Clinical Significance of High-Mobility Group Box 1 Protein (HMGB1) and Nod-Like Receptor Protein 3(NLRP3) in Patients with Ulcerative Colitis. Med Sci Monit. 2020;26:e919530. [26] FREUDENSTEEIN D, LITCHFIELD C, CARAMIA F, et al. TP53 status, patient sex, and the immune response as determinants of lung cancer patient survival. Cancers (Basel). 2020;12(6):1535. [27] DONG Z, CHEN W, CHEN C, et al. CUL3 deficiency causes social deficits and anxiety-like behaviors by impairing excitation-inhibition balance through the promotion of cap-dependent translation. Neuron. 2020;105(3):475-490. [28] EDREES MAH, LUO J, SUN F, et al. Ubc9 deficiency selectively impairs the functionality of common lymphoid progenitors (CLPs) during bone marrow hematopoiesis. Mol Immunol. 2019;114:314-322. [29] 黄聪,彭伟,牟茂婷,等.基于“肢节痹病”的桂枝芍药知母汤的现代药理作用探讨[J].时珍国医国药,2019,30(4):949-950. [30] SAKELLARIOU G, SCIRE CA, ADINOLFI A, et al. Differential diagnosis of inflammatory arthropathies by musculoskeletal ultrasonography: a systematic literature review. Front Med (Lausanne). 2020;7:141. [31] FENG K, CHEN Z, PENGCHENG L, et al. Quercetin attenuates oxidative stress-induced apoptosis via SIRT1/AMPK-mediated inhibition of ER stress in rat chondrocytes and prevents the progression of osteoarthritis in a rat model. J Cell Physiol. 2019;234(10):18192-18205. [32] CHO IA, KIM TH, LIM H, et al. Formononetin antagonizes the interleukin-1β-induced catabolic effects through suppressing inflammation in primary rat chondrocytes. Inflammation. 2019;42(4):1426-1440. [33] FEI J, LIANG B, JIANG C, et al. Luteolin inhibits IL-1β-induced in?ammation in rat chondrocytes and attenuates osteoarthritis progression in a rat model. Biomed Pharmacother. 2019;109:1586-1592. [34] YAMAMOTO S, IWAKUMA T. Regulators of oncogenic mutant TP53 gain of function. Cancers (Basel). 2018;11(1):4. [35] UHLMANN C, KUHN LM, TIGGES J, et al. Efficient modulation of TP53 expression in human induced pluripotent stem cells. Curr Protoc Stem Cell Biol. 2020;52(1):e102. [36] CAO L, BASUDAN A, SIKORA MJ, et al. Frequent amplifications of ESR1, ERBB2 and MDM4 in primary invasive lobular breast carcinoma. Cancer Lett. 2019;461:21-30. [37] KONG Y, WANG Z, HUANG M, et al. CUL7 promotes cancer cell survival through promoting Caspase-8 ubiquitination. Int J Cancer. 2019;145(5):1371-1381. [38] HWANG HS, LEE MH, CHOI MH, et al. NOD2 signaling pathway is involved in fibronectin fragment- induced pro-catabolic factor expressions in human articular chondrocytes. BMB Rep. 2019;52(6):373-378. [39] PAN H, DAI H, WANG L, et al. MicroRNA-410-3p modulates chondrocyte apoptosis and inflammation by targeting high mobility group box 1 (HMGB1) in an osteoarthritis mouse model. BMC Musculoskelet Disord. 2020;21(1):486. [40] 吴剑静,胡洛爽,叶天申,等.桂枝芍药知母汤加减结合微针刀治疗急性痛风性关节炎疗效观察[J].实用中医药杂志,2020,36(3):279-280. [41] LI R, JIA Z, ZHU H. Regulation of Nrf2 signaling. React Oxyg Species (Apex). 2019;8(24):312-322. [42] YANG SC, CHEN PJ, CHANG SH, et al. Luteolin attenuates neutrophilic oxidative stress and inflammatory arthritis by inhibiting Raf1 activity. Biochem Pharmacol. 2018;154:384-396. |
[1] | Wang Jianping, Zhang Xiaohui, Yu Jinwei, Wei Shaoliang, Zhang Xinmin, Xu Xingxin, Qu Haijun. Application of knee joint motion analysis in machanism based on three-dimensional image registration and coordinate transformation [J]. Chinese Journal of Tissue Engineering Research, 2022, 26(在线): 1-5. |
[2] | Tan Xinfang, Guo Yanxing, Qin Xiaofei, Zhang Binqing, Zhao Dongliang, Pan Kunkun, Li Yuzhuo, Chen Haoyu. Effect of uniaxial fatigue exercise on patellofemoral cartilage injury in a rabbit [J]. Chinese Journal of Tissue Engineering Research, 2022, 26(在线): 1-6. |
[3] | Xue Yadong, Zhou Xinshe, Pei Lijia, Meng Fanyu, Li Jian, Wang Jinzi . Reconstruction of Paprosky III type acetabular defect by autogenous iliac bone block combined with titanium plate: providing a strong initial fixation for the prosthesis [J]. Chinese Journal of Tissue Engineering Research, 2022, 26(9): 1424-1428. |
[4] | Zhuang Zhikun, Wu Rongkai, Lin Hanghui, Gong Zhibing, Zhang Qianjin, Wei Qiushi, Zhang Qingwen, Wu Zhaoke. Application of stable and enhanced lined hip joint system in total hip arthroplasty in elderly patients with femoral neck fractures complicated with hemiplegia [J]. Chinese Journal of Tissue Engineering Research, 2022, 26(9): 1429-1433. |
[5] | Jin Tao, Liu Lin, Zhu Xiaoyan, Shi Yucong, Niu Jianxiong, Zhang Tongtong, Wu Shujin, Yang Qingshan. Osteoarthritis and mitochondrial abnormalities [J]. Chinese Journal of Tissue Engineering Research, 2022, 26(9): 1452-1458. |
[6] | Zhang Lichuang, Xu Hao, Ma Yinghui, Xiong Mengting, Han Haihui, Bao Jiamin, Zhai Weitao, Liang Qianqian. Mechanism and prospects of regulating lymphatic reflux function in the treatment of rheumatoid arthritis [J]. Chinese Journal of Tissue Engineering Research, 2022, 26(9): 1459-1466. |
[7] | Jing Jinpeng, Zhang Yue, Liu Xiaomin, Liu Yi. Traditional Chinese medicine injection for promoting blood circulation in prevention of deep vein thrombosis after orthopedic surgery: network meta-analysis [J]. Chinese Journal of Tissue Engineering Research, 2022, 26(9): 1467-1476. |
[8] | Zhang Jichao, Dong Yuefu, Mou Zhifang, Zhang Zhen, Li Bingyan, Xu Xiangjun, Li Jiayi, Ren Meng, Dong Wanpeng. Finite element analysis of biomechanical changes in the osteoarthritis knee joint in different gait flexion angles [J]. Chinese Journal of Tissue Engineering Research, 2022, 26(9): 1357-1361. |
[9] | Bai Zixing, Cao Xuhan, Sun Chengyi, Yang Yanjun, Chen Si, Wen Jianmin, Lin Xinxiao, Sun Weidong. Construction and biomechanical analysis of ankle joint finite element model in gait cycle [J]. Chinese Journal of Tissue Engineering Research, 2022, 26(9): 1362-1366. |
[10] | Wang Baojuan, Zheng Shuguang, Zhang Qi, Li Tianyang. Miao medicine fumigation can delay extracellular matrix destruction in a rabbit model of knee osteoarthritis [J]. Chinese Journal of Tissue Engineering Research, 2022, 26(8): 1180-1186. |
[11] | Wu Bingshuang, Wang Zhi, Tang Yi, Tang Xiaoyu, Li Qi. Anterior cruciate ligament reconstruction: from enthesis to tendon-to-bone healing [J]. Chinese Journal of Tissue Engineering Research, 2022, 26(8): 1293-1298. |
[12] | Hui Xiaoshan, Bai Jing, Zhou Siyuan, Wang Jie, Zhang Jinsheng, He Qingyong, Meng Peipei. Theoretical mechanism of traditional Chinese medicine theory on stem cell induced differentiation [J]. Chinese Journal of Tissue Engineering Research, 2022, 26(7): 1125-1129. |
[13] | Li Jie, Zhang Haitao, Chen Jinlun, Ye Pengcheng, Zhang Hua, Zhou Bengen, Zhao Changqing, Sun Youqiang, Chen Jianfa, Xiang Xiaobing, Zeng Yirong. Anterior cruciate ligament rupture and patellofemoral joint stability before sagittal and axial measurement using MRI [J]. Chinese Journal of Tissue Engineering Research, 2022, 26(6): 969-972. |
[14] | Liu Dongcheng, Zhao Jijun, Zhou Zihong, Wu Zhaofeng, Yu Yinghao, Chen Yuhao, Feng Dehong. Comparison of different reference methods for force line correction in open wedge high tibial osteotomy [J]. Chinese Journal of Tissue Engineering Research, 2022, 26(6): 827-831. |
[15] | Shao Yangyang, Zhang Junxia, Jiang Meijiao, Liu Zelong, Gao Kun, Yu Shuhan. Kinematics characteristics of lower limb joints of young men running wearing knee pads [J]. Chinese Journal of Tissue Engineering Research, 2022, 26(6): 832-837. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||