Effects of estrogen combined with treadmill exercise on bone mass and articular cartilage in ovariectomized mice

doi:10.12307/2022.1010

Chinese Journal of Tissue Engineering Research ›› 2023, Vol. 27 ›› Issue (8): 1166-1171.doi: 10.12307/2022.1010

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Effects of estrogen combined with treadmill exercise on bone mass and articular cartilage in ovariectomized mice

Huang Linke1, 2, 3, Wei Linhua2, 3, 4, Jiang Jie1, 2, 3, Liu Qian2, 3, Chen Weiwei2, 3

1Department of Orthopedics, The Second Affiliated Hospital of Guangxi Medical University, Nanning 530006; 2Regenerative Medicine Research Center, Department of Orthopedics, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021; 3Guangxi Key Laboratory of Regenerative Medicine, Nanning 530021; 4Department of Surgery, Nanning Infectious Disease Hospital Affiliated to Guangxi Medical University, Nanning 530023

Received:2022-01-25 Accepted:2022-03-02 Online:2023-03-18 Published:2022-07-27
Contact: Chen Weiwei, MD, Assistant researcher, Regenerative Medicine Research Center, Department of Orthopedics, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China; Guangxi Key Laboratory of Regenerative Medicine, Nanning 530021, Guangxi Zhuang Autonomous Region, China
About author:Huang Linke, Master, Attending physician, Department of Orthopedics, The Second Affiliated Hospital of Guangxi Medical University, Nanning 530006, Guangxi Zhuang Autonomous Region, China; Regenerative Medicine Research Center, Department of Orthopedics, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China; Guangxi Key Laboratory of Regenerative Medicine, Nanning 530021, Guangxi Zhuang Autonomous Region, China Wei Linhua, Master, Associate chief physician, Regenerative Medicine Research Center, Department of Orthopedics, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China; Guangxi Key Laboratory of Regenerative Medicine, Nanning 530021, Guangxi Zhuang Autonomous Region, China; Department of Surgery, Nanning Infectious Disease Hospital Affiliated to Guangxi Medical University, Nanning 530023, Guangxi Zhuang Autonomous Region, China Huang Linke and Wei Linhua contributed equally to this work.
Supported by:
The 62nd-batch General Project of China Postdoctoral Science Foundation, No. 2017M623296XB (to CWW)

Abstract

Abstract: BACKGROUND: Postmenopausal women are prone to developing osteoporosis and osteoarthritis, and the effect of estrogen replacement therapy combined with treadmill exercise on bone mass and articular cartilage is unclear.
OBJECTIVE: To investigate the effects of exogenous estrogen combined with low intensity treadmill exercise on bone mass and articular cartilage in an ovariectomized mouse model.
METHODS: Thirty-five C57BL/6 mice were randomly divided into five groups: sham group, sham and low intensity treadmill exercise group (sham-sport group), ovariectomized group, ovariectomized and supplying exogenous estrogen group (estrogen group), ovariectomized and supplying exogenous estrogen combined with low intensity treadmill exercise group (estrogen-sport group). The mice were subjected to bilateral ovariectomy or sham operation. The mice in the estrogen and estrogen-sport groups were injected with estrogen every other day after the operation, and the mice in the sham-sport and estrogen-sport groups ran on a treadmill for 6 weeks. The changes in the number of osteoclasts and bone mass in the metaphysis of the distal femur of the mice were observed. Osteoarthritis Research Society International histopathology was used to evaluate the degeneration of knee joint cartilage in mice.
RESULTS AND CONCLUSION: Bone volume per total volume in the estrogen-sport group was significantly higher than that in the ovariectomized group (P < 0.01). There was no statistical difference among the estrogen-sport, sham, sham-sport group, and estrogen groups (P > 0.05). Osteoclast number per bone surface and osteoclast surface per bone surface were significantly decreased in the estrogen-sport group compared with the ovariectomized group (P < 0.01). There was no statistical significance among the estrogen-sport, sham-sport, and estrogen groups (P > 0.05). The knee Osteoarthritis Research Society International histopathology of the estrogen-sport group was significantly higher than that of the other groups (P < 0.01). In conclusion, exogenous estrogen supplementation with low-intensity treadmill exercise can increase bone mass in ovariectomized mice but lead to articular cartilage degeneration.

Key words: estrogen, bone mass, osteoclast, treadmill exercise, ovariectomized mouse, cartilage, osteoarthritis

CLC Number:

Huang Linke, Wei Linhua, Jiang Jie, Liu Qian, Chen Weiwei. Effects of estrogen combined with treadmill exercise on bone mass and articular cartilage in ovariectomized mice[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(8): 1166-1171.

Figures/Tables 4

References

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Effects of estrogen combined with treadmill exercise on bone mass and articular cartilage in ovariectomized mice

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