Medication rules and mechanism of femoral head necrosis: an analysis based on data mining and network pharmacology

doi:10.12307/2021.217

Abstract

Abstract: BACKGROUND: Traditional Chinese medicine is often used as an auxiliary method in the treatment of femoral head necrosis. However, its medication rules, prescription principles, and mechanism of action are still unclear.
OBJECTIVE: To explore the rules of prescriptions for the treatment of femoral head necrosis in the hip-protecting ward of the Third Orthopedics Department of the First Affiliated Hospital of Guangzhou University of Chinese Medicine using data mining, and to explore the mechanism of action of potential new prescription combinations.
METHODS: From January 2017 to December 2019, the prescriptions for the treatment of femoral head necrosis in the Third Orthopedics Department of the First Affiliated Hospital of Guangzhou University of Chinese Medicine were collected. Data mining was conducted through the TCM inheritance auxiliary platform (V2.5), and prescription drugs were analyzed with respect to the frequency of medication uses, drug associations, and potential new prescription combinations. TCMSP, String and KEGG databases were used to analyze the interaction relationship between drug targets in the “new prescription” and to analyze the related pathways obtained.
RESULTS AND CONCLUSION: A total of 126 prescriptions were screened, containing 84 Chinese herbs, and 4 potential new prescription combinations were evolved (prescription 1: peach kernel-astragalus-achyranthes bidentata-angelica-duhuo; prescription 2: Chuanxiong-white peony-dipsacus- atractylodes; prescription 3: Panax notoginseng-poria-rehmannia-salvia-eucommia; prescription 4: safflower-gentiana-psoralen-codonopsis). There were 74 main chemical components (sitosterol α1, gibberellin 17, etc.) of potential new prescription combination 1 (“new prescription”). This study predicted that there were 202 targets for the active ingredients of the “new prescription,” and 121 potential targets for the treatment of femoral head necrosis were obtained after mapping, such as JUN, AKT1, MAPK1, RELA, interleukin-6, etc. The Kyoto Encyclopedia of Genes and Genomes pathway involved in Chinese medicine-disease targets mainly included the MAPK signaling pathway, Toll-like receptors, nuclear factor-κB signaling pathway and hypoxia-inducible factor-1 signaling pathway. The results confirmed that traditional Chinese medicine for the treatment of femoral head necrosis is mainly based on promoting blood circulation to remove blood stasis, nourishing liver and kidney, replenishing qi and blood, supplemented by drugs that clear meridians and dispel rheumatism. The “new prescription” is characterized by multiple components and multiple targets (JUN, AKT1, MAPK1, RELA, interleukin-6, etc.), and its mechanism may be through involvement in promoting angiogenesis, regulating bone metabolism, etc. to regulate the development of femoral head necrosis.

Key words: bone, femur, femoral head necrosis, hip, traditional Chinese medicine, data mining, network pharmacology, molecular mechanism, signaling pathway

CLC Number:

Wang Ding, Lin Tianye, Chen Weijian, Chen Jianfeng, Ou Huizhi, Li Hongzhu, Yang Peng, Wei Qiushi, He Wei, Feng Zongquan. Medication rules and mechanism of femoral head necrosis: an analysis based on data mining and network pharmacology[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(32): 5148-5154.

Figures/Tables 11

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