中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (14): 2648-2651.doi: 10.3969/j.issn.1673-8225.2012.14.037

• 干细胞临床实践 clinical practice of stem cells • 上一篇    下一篇

急性淋巴细胞白血病患者外周血CD4+CD25+T调节细胞与白细胞介素2、白细胞介素10、转化生长因子β的检测*

 

巫翠萍1，覃  西1，巫翠云2，朱  洪1，周海燕1   

  1. 1海南医学院附属医院，海南省海口市  570102；2海口市人民医院，海南省海口市570208
  • 收稿日期:2011-08-08 修回日期:2011-10-30 出版日期:2012-04-01 发布日期:2012-04-01
  • 通讯作者: 覃西，教授，海南医学院附属医院，海南省海口市 570102 qinxi99@21cn.com
  • 作者简介:巫翠萍，女，1962年生，海南省海口市人，汉族，2002年海南医学院毕业，副主任检验师，主要从事临床血液病学检验及流式细胞术的应用与研究。wucuiping2007@163.com
  • 基金资助:

    海南省卫生厅科研立项课题(琼卫2008-55)。

Detection of interleukin 2, interleukin 10, transforming growth factor β and CD4+CD25+ regulatory T cells in peripheral blood of patients with acute lymphoblastic leukemia 

Wu Cui-Ping1, Qin Xi1, Wu Cui-yun2, Zhu Hong1, Zhou Hai-yan1   

  1. 1The Affiliated Hospital of Hainan Medical College, Haikou  570102, Hainan Province, China; 2People’s Hospital of Haikou, Haikou  570208, Hainan Province, China
  • Received:2011-08-08 Revised:2011-10-30 Online:2012-04-01 Published:2012-04-01
  • Contact: Qin Xi, Professor, the Affiliated Hospital of Hainan Medical College, Haikou 570102, Hainan Province, China qinxi99@21cn.com
  • About author:Wu Cui-Ping, Associate chief technician, the Affiliated Hospital of Hainan Medical College, Haikou 570102, Hainan Province, China wucuiping2007@163.com
  • Supported by:
    Reaearch Project Topics of HealthyDepartment of Hainan Province, No. Qiongwei2008-55*
     

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摘要：

背景：目前急性淋巴细胞白血病的病因尚未完全清楚，而CD4+CD25+T调节细胞对机体的免疫反应可能起着一定的调节作用。
目的：观察急性淋巴细胞白血病患者外周血CD4+CD25+T调节细胞分泌白细胞介素2、白细胞介素10及转化生长因子β的情况。
方法：采用密度梯度离心法分离22例B细胞系列的急性淋巴细胞白血病(B-ALL)患者、13例T细胞系列的急性淋巴细胞白血病(T-ALL)患者和18名正常人外周血单个核细胞。采用免疫磁珠法分离出CD4+CD25+T调节细胞进行培养，同时用双抗体夹心酶联免疫吸附试验检测细胞培养上清中白细胞介素2、白细胞介素10及转化生长因子β的水平。
结果与结论：B-ALL患者和T-ALL患者外周血CD4+CD25+T调节细胞培养上清中白细胞介素10和转化生长因子β水平显著高于正常人水平，而白细胞介素2水平显著低于正常人水平(P < 0.05)；B-ALL和T-ALL患者的CD4+CD25+T调节细胞培养上清中3种因子表达差异无显著性意义(P > 0.05)。提示CD4+CD25+T调节细胞可能通过增加分泌白细胞介素10和转化生长因子β、减少分泌白细胞介素2等干扰抗肿瘤的免疫反应。
 

关键词: 急性淋巴细胞白血病, CD4+CD25+T调节细胞, 白细胞介素2, 白细胞介素10, 转化生长因子&beta

Abstract:

BACKGROUND: At present, the cause of acute lymphoblastic leukemia (ALL) has not yet entirely understood, CD4+CD25+ regulatory T cells (Treg) may have certain regulatory effect on immune response in pathogenic process of ALL.
OBJECTIVE: To study the levels of interleukin 2 (IL-2), interleukin 10 (IL-10), transforming growth factor (TGF-β) of CD4+CD25+ regulatory T cells (Treg) in peripheral blood of patients with ALL.
METHODS: The peripheral blood mononuclear cells (PBMC) were isolated with density gradient centrifugation from 22 B cell series ALL (B-ALL) patients, 13 T cell series ALL (T-ALL) patients and 18 normal people. Immunomagnetic bead separation was used to separate CD4+CD25+Treg cells, and the levels of IL-2, IL-10 and TGF-β in the cells culture supernatant were detected by using enzyme-linked immunosorbent assay (ELISA).
RESULTS AND CONCLUSION: The levels of IL-10 and TGF-β in the cells culture supernatant of B-ALL patients and T-ALL patients were significantly higher than those in normal people, while the level of IL-2 was significantly lower than that in normal people (P < 0.05). There was no significant difference between B-ALL and T-ALL patients in the IL-2, IL-10 and TGF-β levels of the cells culture supernatant (P > 0.05). CD4+CD25+Treg cells might interfere the immunologic reaction of antitumor by increasing the IL-10 and TGF-β levels and decreasing the IL-2 level.
 

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