中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (42): 7861-7865.doi: 10.3969/j.issn.2095-4344.2012.42.014

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

一种新型小鼠重症急性胰腺炎模型的制作及评估

朱长炎1，赵海峰1，曾宪有1，吴卫真2   

  1. 1福建医科大学福总临床医学院，福建省福州市 350025
    2福建医科大学福总临床医学院，解放军南京军区福州总医院泌尿外科，福建省福州市 350025
  • 收稿日期:2012-02-23 修回日期:2012-03-30 出版日期:2012-10-14 发布日期:2013-04-08
  • 通讯作者: 吴卫真，博士，教授，福建医科大学福总临床医学院，解放军南京军区福州总医院泌尿外科，福建省福州市 350025 wwzhen@medmail.com.cn
  • 作者简介:朱长炎★，男，1982年生，江西省万年县人，汉族，福建医科大学在读硕士。

Preparation and evaluation of a new type model of severe acute pancreatitis in mice

Zhu Chang-yan1, Zhao Hai-feng1, Zeng Xian-you1, Wu Wei-zhen2   

  1. 1Key Laboratory of Biology Transplantation, Fuzhou General Clinical Medical College of Fujian Medical University, Fuzhou 350025, Fujian Province, China
    2 Fuzhou General Clinical Medical College of Fujian Medical University, Fuzhou 350025, Fujian Province, China
  • Received:2012-02-23 Revised:2012-03-30 Online:2012-10-14 Published:2013-04-08
  • Contact: Wu Wei-zhen, Doctor, Professor, Fuzhou General Clinical Medical College of Fujian Medical University, Fuzhou 350025, Fujian Province, China wwzhen@medmail.com.cn
  • About author:Zhu Chang-yan★, Studying for master’s degree, Key Laboratory of Biology Transplantation, Fuzhou General Clinical Medical College of Fujian Medical University, Fuzhou 350025, Fujian Province, China

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1267

被引次数

5

摘要：

背景：目前有关小鼠重症急性胰腺炎模型有多次剂量注射雨蛙素法、高剂量L-精氨酸诱导法和胆碱缺乏食物喂养等，但有各自应用的局限性，不能较为广泛使用。
目的：探索并建立一种新型小鼠重症急性胰腺炎动物模型。
方法：雄性ICR小鼠40只随机分成2组，假手术组小鼠仅行开腹和关腹，模型组小鼠直视下逆行胆胰管注射30 g/L牛黄胆酸钠建立重症急性胰腺炎模型。所有小鼠在注射后6，12，24，48 h麻醉下处死进行指标检测。
结果与结论：各时间点模型组血清淀粉酶、谷丙转氨酶、血肌酐及乳酸脱氢酶均显著高于假手术组(P < 0.05)，胰腺组织病理评分(水肿，炎性细胞浸润，坏死)显著高于假手术组(P < 0.05)，伴有肺组织病理轻度改变。结果表明，在直视下逆行注射牛黄胆酸钠诱发小鼠重症急性胰腺炎模型，具有快捷，可重复性好而又稳定的优点，且符合临床病变特征。

关键词: 重症急性胰腺炎, 牛黄胆酸钠, 小鼠, 淀粉酶, 谷丙转氨酶, 血肌酐, 乳酸脱氢酶, 病理, 动物模型

Abstract:

BACKGROUND: At present, there are several methods for preparing a model of severe acute pancreatitis (SAP) in mice, including Hyla method with multiple injection, L-arginine-induced method with high dose and choline deficiency food feeding. But these methods all have their limiations, and therefore, they cannot be used widely.
OBJECTIVE: To explore and establish a new type model of SAP in mice.
METHODS: Forty adult male ICR mice were collected randomly and divided into two groups (each of 20 mice): sham operation group and model group. The mice of the sham operation group were only performed laparotomy and closure. The mice of the model group were injected retrogradely into biliopancreatic duct with 3% sodium taurocholate under macroscopic observation. All mice were killed under anesthesia at hours 6, 12, 24 and 48 after injection. Serum amylase, alanine aminotransferase, creatinine and lactate dehydrogenase were assayed. Histological scores of pancreas and lung of the two groups were examined under microscope.
RESULTS AND CONCLUSION: Activities of serum amylase, serum alanine aminotransferase, serum creatinine and serum lactate dehydrogenase of the model group were significantly higher than those of the sham operation group at each time point (P < 0.05). Histopathological scores: interstitial edema, inflammatory cells (mononuclear and neutrophil cells) infiltration, necrosis as well as parenchymal hemorrahages in the model group were higher than those in the sham operation group (P < 0.05), and lesions of other organs including the lung, liver and kidney were found. These results suggest that compared with other methods, the mice model of SAP induced by sodium taurocholate under macroscopic observation is easy, reproducible and stable, and is in accordance with clinical diseases.

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