中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (45): 8430-8434.doi: 10.3969/j.issn.2095-4344.2012.45.013

• 脂肪干细胞 adipose-derived stem cells • 上一篇    下一篇

人脂肪间充质干细胞的免疫调节作用

王建军1，樊 艳2，马爱群2，高明暄3   

  1. 1兰州市第一人民医院骨科，甘肃省兰州市 730050
    2西安交通大学医学院第一附属医院心血管内科，陕西省西安市 710061
    3解放军兰州军区兰州总医院创伤骨科，甘肃省兰州市 730050
  • 收稿日期:2012-02-22 修回日期:2012-05-09 出版日期:2012-11-04 发布日期:2013-03-16
  • 作者简介:王建军★，男，1980年生，甘肃省兰州市人，汉族，2009年兰州大学毕业，硕士，主治医师，主要从事骨科基础与临床的研究。 fancy677@163.com

Immune regulatory effect of human adipose-derived mesenchymal stem cells

Wang Jian-jun1, Fan Yan2, Ma Ai-qun2, Gao Ming-xuan3   

  1. 1Department of Orthopedics, the First People’s Hospital of Lanzhou, Lanzhou 730050, Gansu Province, China
    2Department of Cardiovascular Medicine, the First Affiliated Hospital of Medical College of Xi’an Jiaotong University, Xi’an 710061, Shanxi Province, China
    3Department of Orthopedic Trauma, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu Province, China
  • Received:2012-02-22 Revised:2012-05-09 Online:2012-11-04 Published:2013-03-16
  • About author:Wang Jian-jun★, Master, Attending physician, Department of Orthopedics, the First People’s Hospital of Lanzhou, Lanzhou 730050, Gnasu Province, China fancy677@163.com

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561

被引次数

10

摘要：

背景：脂肪间充质干细胞的免疫调节作用及其机制如何，目前了解甚少。
目的：观察人脂肪间充质干细胞体外对淋巴细胞增殖、细胞亚群和分泌细胞因子水平的影响，以了解其免疫调节作用。
方法：分离培养成人脂肪间充质干细胞，流式细胞仪鉴定细胞表型，分别成骨、成心肌细胞诱导，免疫细胞化学染色对诱导后的细胞进行鉴定。分离培养人外周血淋巴细胞，按不同浓度(1∶1，1∶10，1∶100)与脂肪间充质干细胞共培养，并添加植物血凝素刺激，同时设立对照组，3 d后收集上清。
结果与结论：脂肪间充质干细胞与淋巴细胞共培养后检测3种浓度组淋巴细胞的A值明显低于阳性对照组，其中1∶1浓度组最低(P < 0.05)；CD4+CD25+Tregs亚群比例明显高于阳性对照组，且1∶1浓度组最高；细胞因子白细胞介素2、白细胞介素4、γ-干扰素水平明显减低，但白细胞介素10水平升高，且具有一定的浓度依赖性(P < 0.05)。提示脂肪间充质干细胞在体外能明显抑制淋巴细胞的增殖，这种抑制作用可能与其增加CD4+CD25+Tregs亚群数量，以及改变淋巴细胞分泌细胞因子的水平有关。

关键词: 淋巴细胞, 脂肪间充质干细胞, 免疫调节, 移植物抗宿主病, 干细胞

Abstract:

BACKGROUND: We know little about the immune regulatory effect of adipose-derived mesenchymal stem cells.
OBJECTIVE: To investigate the influence of adult adipose-derived mesenchymal stem cells on lymphocyte proliferation, cells subsets and the level of cell factors in vitro, in order to understand the immune regulatory effect.
METHODS: Adipose-derived mesenchymal stem cells were isolated from adult adipose tissue. The phenotype was identified with the flow cytometry. Adipose-derived mesenchymal stem cells were induced into osteoblasts and myocardial cells, respectively, and identified with the immunocytochemistry staining. The human peripheral blood lymphocytes were separated and cultured with adipose-derived mesenchymal stem cells according to different concentrations (1:1, 1:10, 1:100), and added the plant haemagglutinin for simulation. The control group was set up, and after 3 days, the culture fluid was collected.
RESULTS AND CONCLUSION: The absorbance value of adipose-derived mesenchymal stem cells in the treatment group after co-cultured with lymphocytes with three different concentrations was significantly lower than that in the control group, and 1:1 concentration had the lowest absorbance value (P < 0.05); the proportion of CD4+CD25+ Tregs in the treatment groups was significantly higher than that in the control group, and highest in the 1:1 concentration group. The cell factors of interleukin-2, interleukin-4 and γ-interferon were obviously reduced in the treatment groups, while the interleukin-10 level was increased, and showed a certain concentration-dependent manner (P < 0.05). Adipose-derived mesenchymal stem cells can significantly suppress the proliferation of lymphocyte in vitro. The inhibition effect may relate with the increasing of CD4+CD25+ Tregs subsets, and relate with the changing of cytokine secretion of lymphocyte.

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