中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (1): 7-11.

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

损伤脊髓匀浆上清对骨髓间充质干细胞分泌髓鞘前脂蛋白、脑源性神经营养因子的影响

刘  然1，2，范东艳3，金  鹏4，范洪学1，王  苹4   

  1. 1吉林大学公共卫生学院，吉林省长春市 130021
    2 长春市中心血站，吉林省长春市 130031
    3西藏大学医学院，西藏自治区拉萨市  850000
    4吉林大学第一医院耳鼻喉-头颈外科，吉林省长春市  130021
  • 收稿日期:2010-09-20 修回日期:2010-11-22 出版日期:2011-01-01 发布日期:2011-01-01
  • 通讯作者: 王苹，博士，副研究员，吉林大学第一医院耳鼻喉-头颈外科，吉林省长春市 130021 wangping99@hotmail.com
  • 作者简介:刘然★，男，1977年生，吉林省长春市人，汉族，2005年日本东京圣德大学毕业，硕士，主治医师，主要从事干细胞应用的研究。 lr770920@163.com

Effects of injured spinal cord extracts on brain-derived neurotropic factor and myelin proteolipid protein in bone marrow mesenchymal stem cells

Liu Ran1, 2, Fan Dong-yan3, Jin Peng4, Fan Hong-xue1, Wang Ping4   

  1. 1College of Public Health, Jilin University, Changchun  130021, Jilin Province, China
    2Changchun Blood Center, Changchun  130031, Jilin Province, China
    3Medical College of Xizang University, Lasa  850000, Xizang Autonomous Region, China
    4Department of Otolaryngology, Head and Neck Surgery, First Hospital, Jilin University, Changchun  130021, Jilin Province, China
  • Received:2010-09-20 Revised:2010-11-22 Online:2011-01-01 Published:2011-01-01
  • Contact: Wang Ping, Doctor, Associate researcher, Department of Otolaryngology, Head and Neck Surgery, First Hospital, Jilin University, Changchun 130021, Jilin Province, China wangping99@hotmail.com
  • About author:Liu Ran★, Master, Attending physician, College of Public Health, Jilin University, Changchun 130021, Jilin Province, China; Changchun Blood Center, Changchun 130031, Jilin Province, China lr770920@163.com

PDF

409

被引次数

9

摘要：

背景：损伤脊髓匀浆上清成分复杂，其中不仅存在多种化学物质，而且也存在着多种细胞因子，这些物质能否影响骨髓间充质干细胞的增殖分化和分泌功能还不清楚。
目的：探讨损伤脊髓匀浆上清成分对骨髓间充质干细胞分泌的脑源性神经营养因子和髓鞘前脂蛋白的影响。
方法：贴壁法分离纯化Wistar大鼠骨髓间充质干细胞，稳定传到第3代后，分别用正常和损伤的Wistar大鼠脊髓匀浆上清诱导培养20 d。免疫荧光染色检测神经元特异性烯醇化酶阳性细胞，ELISA法检测培养液内髓鞘前脂蛋白、脑源性神经营养因子的含量，即时定量-PCR检测髓鞘前脂蛋白mRNA、脑源性神经营养因子mRNA水平。
结果与结论：损伤脊髓匀浆上清液诱导培养骨髓间充质干细胞后，神经元特异性烯醇化酶阳性细胞率和培养液内脑源性神经营养因子、髓鞘前脂蛋白的含量在各个时间点均较正常脊髓匀浆上清液对骨髓间充质干细胞培养组高。提示，损伤的脊髓匀浆上清液能够诱导骨髓间充质干细胞分泌脑源性神经营养因子、髓鞘前脂蛋白，有利于向神经细胞方向分化。

关键词: 脑源性神经营养因子, 髓鞘前脂蛋白, 骨髓间充质干细胞, 脊髓匀浆上清液, 损伤脊髓

Abstract:

BACKGROUND: The component of injured spinal cord extracts is complex and includes some chemical substances or cytokines. It remains unclear if these factors may influence cellular proliferation and secretion of bone marrow mesenchymal stem cells (BMSCs) or not.  
OBJECTIVE: To investigate the effect of injured spinal cord extracts on secretion of brain-derived neurotropic factor (BDNF) or myelin proteolipid protein (PLP) in BMSCs.
METHODS: BMSCs were obtained from Wistar rats. After 3 generation of cell proliferation, BMSCs were induced with normal or injured spinal cord extracts for 20 days. Neuron specific enolase (NSE) positive cells were detected by immunofluorescence staining. Levels of BDNF and PLP in BMSCs cultured supernatant were evaluated using enzyme linked immunosorbent assay (ELISA) and BDNF and PLP mRNA was determined by real time polymerase chain reaction assay.
RESULTS AND CONCLUSION: The rate of NSE positive cells and levels of BDNF and PLP that were treated with injured spinal cord extracts were greater significantly at different cultured time points than normal spinal cord extracts. These results showed that the injured spinal cord extracts could induce secretion of BDNF and PLP in BMSCs that is to the benefit of cellular differentiation into neuronal cells.

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