关键词: 囊胚培养, 囊胚移植, 临床妊娠, 内细胞团, 滋养层细胞

Abstract:

BACKGROUND: The blastocysts were transferred into the uterine cavity following blastocyst culture. It may shorten the period of embryo development and implantation. On the other hand, uterine contractility decreases gradually. Thus, it would decrease the opportunity of the embryo implantation in vitro. The blastocyst transfer is fit for the physiological function.
OBJECTIVE: To observe the pregnancy rate following blastocyst transfer.
METHODS: Patients underwent superovulation with gonadotropin releasing hormone agonist and gonadotropin. Oocyte-cumulus-corano complex were isolated from follicular fluid and immediately placed in human insemination media supplemented with human serum albumin. Fertilization was assessed and scored at 16-18 hours after insemination and transferred to cleavage media. Development of embryo and number f blastomere at 3 days were assessed and graded.
RESULTS AND CONCLUSION: Only one patient was pregnant after embryo transfer among those 33 patients who had no blastocyst was transferred. The clinical pregnancy rate of 238 patients after blastocyst transfer was 49.16%. It was no statistically different of the success rate among the different maternal age. 122 patients were transferred at least one good-quality blastocyst, the clinical pregnancy rate was 56.56%. 116 patients were transferred poor-quality blastocyst, the clinical pregnancy rate was 41.38%. It showed that it is no effect of the maternal age on the clinical pregnancy rate after blastocyst transfer. It may be cancelled if the patient was no blastocyst. But the blastocyst should be transferred whether there is good-quality blastocyst or not.