中国组织工程研究

中国组织工程研究 ›› 2026, Vol. 30 ›› Issue (26): 6719-6728.doi: 10.12307/2026.361

• 功能性生物材料Functional biomaterials • 上一篇    下一篇

多功能复合涂层改性聚醚醚酮的促成骨与抗菌性能

张慧芳1，2，3，李函锴1，2，3，黄浩哲1，2，3，刘  敏1，2，3，王  频1，2，3，黄海霞1，2，3，孙黎波4，兰玉燕1，2，3   

  1. 1西南医科大学附属口腔医院口腔修复科，四川省泸州市   646000；2口颌面修复重建和再生泸州市重点实验室，四川省泸州市  646000；3西南医科大学口腔医学研究所，四川省泸州市   646000；4泸州市人民医院口腔科，四川省泸州市   646000
  • 接受日期:2025-08-30 出版日期:2026-09-18 发布日期:2026-03-10
  • 通讯作者: 兰玉燕，硕士，副教授，西南医科大学附属口腔医院口腔修复科，四川省泸州市 646000；口颌面修复重建和再生泸州市重点实验室，四川省泸州市 646000；西南医科大学口腔医学研究所，四川省泸州市 646000 孙黎波，硕士，副教授，副主任医师，泸州市人民医院口腔科，四川省泸州市 646000
  • 作者简介:张慧芳，1997年生，江西省赣州市人，汉族，西南医科大学在读硕士，主要从事口腔修复与种植的基础研究工作。
  • 基金资助:
    四川省医学会青年创新科研课题项目(Q22064)，项目负责人：兰玉燕；泸州市科技局应用研究基础项目(2023JYJ053)，项目负责人：兰玉燕；泸州市科技计划资助项目(2024JYJ087)，项目负责人：孙黎波；泸州市医学会科研项目(2024-YXXM-010)，项目负责人：孙黎波

Osteogenic and antibacterial properties of polyether ether ketone modified by multifunctional composite coating

Zhang Huifang1, 2, 3, Li Hankai1, 2, 3, Huang Haozhe1, 2, 3, Liu Min1, 2, 3, Wang Pin1, 2, 3, Huang Haixia1, 2, 3, Sun Libo4, Lan Yuyan1, 2, 3   

  1. 1Department of Prosthodontics, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou 646000, Sichuan Province, China; 2Luzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Southwest Medical University, Luzhou 646000, Sichuan Province, China; 3Institute of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China; 4Department of Stomatology, Luzhou People’s Hospital, Luzhou 646000, Sichuan Province, China 
  • Accepted:2025-08-30 Online:2026-09-18 Published:2026-03-10
  • Contact: Lan Yuyan, MS, Associate professor, Department of Prosthodontics, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Luzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Institute of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China Sun Libo, MS, Associate professor, Associate chief physician, Department of Stomatology, Luzhou People’s Hospital, Luzhou 646000, Sichuan Province, China
  • About author:Zhang Huifang, Master candidate, Department of Prosthodontics, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Luzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Institute of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Supported by:
    Youth Innovation Research Project of Sichuan Medical Association, No. Q22064 (to LYY); Luzhou Science and Technology Bureau Applied Research Basic Project, No. 2023JYJ053 (to LYY); Luzhou Science and Technology Plan Funding Project, No. 2024JYJ087 (to SLB); Scientific Research Project of Luzhou Medical Association, No. 2024-YXXM-010 (to SLB)

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摘要：

文题释义：
聚醚醚酮：是一种由主链上单酮键和双醚键重复单元组成的半结晶聚合物，弹性模量接近人类皮质骨，具有良好的生物安全性、生物力学性能、自然光透过率、耐腐蚀等优点，可用于航空、军事、电子、石油化工等领域，在医疗领域可用于人工骨替代材料、牙种植体等。
掺锶羟基磷灰石：也称锶取代羟基磷灰石或锶掺杂改性的羟基磷灰石，其中的羟基磷灰石是人类骨骼和牙齿组织的主要无机成分，化学式为Ca10(PO4)6(OH)2。锶作为一种微量元素通过刺激骨形成和抑制骨吸收在骨代谢中发挥重要作用，当锶离子和钙离子同时存在时，锶离子可以部分取代钙离子，增强骨诱导活性。

背景：聚醚醚酮的固有生物活性不足，作为骨植入材料存在骨结合不良和种植体相关感染风险，开发兼具成骨和抗菌功能的表面改性策略对于提升聚醚醚酮植入性能具有重要的临床意义。
目的：分析负载聚多巴胺修饰掺锶羟基磷灰石-银复合涂层聚醚醚酮的生物相容性、促成骨和抗菌作用。
方法：①在聚醚醚酮表面制备聚多巴胺-羟基磷灰石复合涂层，记为PEEK@PDA-HA；按照Sr2+/(Sr2++Ca2+)分别为0%，5%，10%，20%的比例，在聚醚醚酮表面制备负载聚多巴胺-掺锶羟基磷灰石-银复合涂层，分别记为PEEK@PDA-HA-Ag、PEEK@PDA-5Sr/HA-Ag、PEEK@PDA-10Sr/HA-Ag、PEEK@PDA-20Sr/HA-Ag。将MC3T3-E1细胞分别与聚醚醚酮、PEEK@PDA-HA-Ag、PEEK@PDA-5Sr/HA-Ag、PEEK@PDA-10Sr/HA-Ag、PEEK@PDA-20Sr/HA-Ag共培养，通过细胞增殖、活死染色与黏附检测结果筛选最佳材料用于后续实验。表征PEEK@PDA-10Sr/HA-Ag的表面形貌与水接触角。②将MC3T3-E1细胞分别接种于聚醚醚酮、PEEK@PDA-HA、PEEK@PDA-HA-Ag、PEEK@PDA-10Sr/HA-Ag表面，成骨诱导后分别进行碱性磷酸酶染色、茜素红染色与骨钙素免疫荧光染色，检测材料的促成骨分化性能。③将大肠杆菌(或金黄色葡萄球菌)分别与聚醚醚酮、PEEK@PDA-HA、PEEK@PDA-HA-Ag、PEEK@PDA-10Sr/HA-Ag共培养，通过琼脂平板计数法和细菌活死染色检测材料的抗菌性能。
结果与结论：①细胞增殖、活死染色与黏附检测结果显示，PEEK@PDA-10Sr/HA-Ag促MC3T3-E1细胞增殖效果最好，同时该材料表面黏附的MC3T3-E1细胞形态良好且伸出较多丝状伪足，可用于后续实验。扫描电镜下可见PEEK@PDA-10Sr/HA-Ag表面粗糙、凹凸不平，附着大量球状纳米颗粒团块。相较于聚醚醚酮，PEEK@PDA-10Sr/HA-Ag表面水接触角减小，亲水性增强。②碱性磷酸酶染色、茜素红染色、骨钙素免疫荧光染色显示，PEEK@PDA-10Sr/HA-Ag促成骨作用最强。③细菌平板计数和活死染色显示，相较于其他3组材料，PEEK@PDA-10Sr/HA-Ag可有效抑制大肠杆菌、金黄色葡萄球菌的生长。④结果表明，负载聚多巴胺修饰掺锶羟基磷灰石-银复合涂层聚醚醚酮具有良好的生物相容性、促成骨和抗菌作用。

https://orcid.org/0009-0004-1180-1388 (张慧芳)

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料；口腔生物材料；纳米材料；缓释材料；材料相容性；组织工程

关键词: 聚醚醚酮, 复合涂层, 聚多巴胺, 锶, 羟基磷灰石, 掺锶羟基磷灰石, 银

Abstract: BACKGROUND: Polyether ether ketone (PEEK) has insufficient inherent biological activity. As a bone implant material, there are risks of poor osseointegration and implant-associated infections. The development of PEEK surface modification strategies with both osteogenic and antibacterial functions has important clinical significance for improving the implantable performance of PEEK.
OBJECTIVE: To analyze the biocompatibility, osteogenic and antibacterial effects of polydopamine-modified strontium-doped hydroxyapatite-silver composite coating PEEK. 
METHODS: (1) A polydopamine-hydroxyapatite composite coating was prepared on the surface of PEEK, recorded as PEEK@PDA-HA. According to the ratio of Sr2+/(Sr2++Ca2+) of 0%, 5%, 10%, and 20%, respectively, a polydopamine-strontium-doped hydroxyapatite-silver composite coating was prepared on the surface of PEEK, recorded as PEEK@PDA-HA-Ag, PEEK@PDA-5Sr/HA-Ag, PEEK@PDA-10Sr/HA-Ag, and PEEK@PDA-20Sr/HA-Ag, respectively. MC3T3-E1 cells were co-cultured with PEEK, PEEK@PDA-HA-Ag, PEEK@PDA-5Sr/HA-Ag, PEEK@PDA-10Sr/HA-Ag, and PEEK@PDA-20Sr/HA-Ag. The best materials were selected for subsequent experiments through cell proliferation, live-dead staining, and adhesion test results. The surface morphology and water contact angle of PEEK@PDA-10Sr/HA-Ag were characterized. (2) MC3T3-E1 cells were inoculated on the surfaces of PEEK, PEEK@PDA-HA, PEEK@PDA-HA-Ag, and PEEK@PDA-10Sr/HA-Ag. Alkaline phosphatase staining, alizarin red staining, and osteocalcin immunofluorescence staining were performed after osteogenic induction to detect the osteogenic differentiation performance of the materials. (3) Escherichia coli (or Staphylococcus aureus) was co-cultured with PEEK, PEEK@PDA-HA, PEEK@PDA-HA-Ag, and PEEK@PDA-10Sr/HA-Ag. The antibacterial properties of the materials were detected by agar plate counting method and bacterial live and dead staining. 
RESULTS AND CONCLUSION: (1) The results of cell proliferation, live and dead staining, and adhesion detection showed that PEEK@PDA-10Sr/HA-Ag had the best effect on promoting MC3T3-E1 cell proliferation. At the same time, the MC3T3-E1 cells adhered to the surface of the material had good morphology and extended more filopodia, which could be used for subsequent experiments. Scanning electron microscopy showed that the surface of PEEK@PDA-10Sr/HA-Ag was rough and uneven, with a large number of spherical nanoparticles attached. Compared with PEEK, the water contact angle of the surface of PEEK@PDA-10Sr/HA-Ag was reduced, and the hydrophilicity was enhanced. (2) Alkaline phosphatase staining, alizarin red staining, and osteocalcin immunofluorescence staining showed that PEEK@PDA-10Sr/HA-Ag had the strongest osteogenic effect. (3) Bacterial plate counts and live-dead staining showed that PEEK@PDA-10Sr/HA-Ag could effectively inhibit the growth of Escherichia coli and Staphylococcus aureus compared with the other three groups of materials. (4) The results showed that PEEK loaded with polydopamine modified strontium-doped hydroxyapatite-silver composite coating had good biocompatibility, osteogenic and antibacterial effects.


Key words: polyether ether ketone, composite coating, polydopamine, strontium, hydroxyapatite, strontium-doped hydroxyapatite, silver

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