中国组织工程研究

中国组织工程研究 ›› 2026, Vol. 30 ›› Issue (24): 6225-6230.doi: 10.12307/2026.174

• 肌肉肌腱韧带组织构建 tissue construction of the muscle, tendon and ligament • 上一篇    下一篇

内侧副韧带与外侧副韧带力学差异及弹性蛋白降解的影响

徐洪璋1，黄  波1，赵东良2，胡  赢1，乔  丹3，邓羽平1，2，4   

  1. 1南方医科大学中西医结合医院骨伤科，广东省广州市   510315；2北京大学深圳研究生院，深圳湾实验室，广东省深圳市   518132；3浙江大学医学院附属邵逸夫医院病理科，浙江省杭州市   310016；4南方医科大学基础医学院人体解剖学国家重点学科，广东省医学生物力学重点实验室，广东省医学3D打印应用转化工程技术研究中心，广东省广州市   510515
  • 收稿日期:2025-06-03 修回日期:2025-09-15 出版日期:2026-08-28 发布日期:2026-01-29
  • 通讯作者: 徐洪璋，医学博士，副主任医师，南方医科大学中西医结合医院骨伤科，广东省广州市 510315
  • 作者简介:徐洪璋，男，1974年生，江西省鄱阳县人，汉族，医学博士，副主任医师，主要从事数字骨科与生物力学方面的研究。
  • 基金资助:
    国家自然科学基金青年项目(82305261)，项目负责人：邓羽平；中国博士后科学基金项目(2022M711533)，项目负责人：邓羽平；国家自然科学基金青年项目(12202017)，项目负责人：赵东良；深圳市科技计划资助项目(JCYJ20210324130401005)，项目负责人：赵东良；深圳市医学研究专项资金项目(深医专项A2303037)，项目负责人：赵东良；深圳湾实验室启动基金项目(QH3003，21300021)，项目负责人：赵东良；深圳市优秀科技创新人才培养项目(2023511739，多尺度-多场耦合心肌动力学)，项目负责人：赵东良；深圳湾实验室超算中心项目，项目负责人：赵东良

Mechanical differences between medial collateral ligament and lateral collateral ligament and influence of elastin degradation

Xu Hongzhang1, Huang Bo1, Zhao Dongliang2, Hu Ying1, Qiao Dan3, Deng Yuping1, 2, 4   

  1. 1Department of Orthopedics and Traumatology, Hospital of Integrated Traditional Chinese and Western Medicine, Southern Medical University, Guangzhou 510315, Guangdong Province, China; 2Shenzhen Bay Laboratory, Shenzhen Graduate School of Peking University, Shenzhen 518132, Guangdong Province, China; 3Department of Pathology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, Zhejiang Province, China; 4National Key Discipline of Human Anatomy, Guangdong Provincial Key Laboratory of Medical Biomechanics, Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • Received:2025-06-03 Revised:2025-09-15 Online:2026-08-28 Published:2026-01-29
  • Contact: Xu Hongzhang, Department of Orthopedics and Traumatology, Hospital of Integrated Traditional Chinese and Western Medicine, Southern Medical University, Guangzhou 510315, Guangdong Province, China
  • About author:Xu Hongzhang, MD, Associate chief physician, Department of Orthopedics and Traumatology, Hospital of Integrated Traditional Chinese and Western Medicine, Southern Medical University, Guangzhou 510315, Guangdong Province, China
  • Supported by:
    National Natural Science Foundation of China (Youth Project), Nos. 82305261 (to DYP) and 12202017 (to ZDL); China Postdoctoral Science Foundation Project, No. 2022M711533 (to DYP); Shenzhen Municipal Science and Technology Plan Project, No. JCYJ20210324130401005 (to ZDL); Shenzhen Municipal Medical Research Special Fund Project, No. A2303037 (to ZDL); Shenzhen Bay Laboratory Startup Fund Project, No. QH3003, 21300021 (to ZDL); Shenzhen Municipal Outstanding Science and Technology Innovation Talent Cultivation Project, No. 2023511739 (to ZDL); Shenzhen Bay Laboratory Supercomputing Center Project (to ZDL)

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摘要：



文题释义：
应力-应变曲线：是一种用来描述材料在受力过程中的力学行为的图形化工具，表示了材料在外力作用下应力(单位面积上所承受的力)和应变(材料变形的程度)之间的关系。
刚度：是一种结构特性，表示结构在外力作用下抵抗变形的能力。刚度取决于材料的模量以及结构的形状和尺寸。

背景：内侧副韧带和外侧副韧带作为膝关节的重要稳定器分别承担着限制膝关节外翻与内翻的关键功能，但是内侧副韧带和外侧副韧带之间的力学差异、微观结构特点和弹性蛋白降解对它们力学性能的影响尚不清楚。
目的：比较内侧副韧带和外侧副韧带的力学差异，量化胶原纤维排布的结构特点，探讨弹性蛋白降解对两韧带力学性能的影响。
方法：获取成年猪左侧内侧副韧带和外侧副韧带，冷冻保存后解冻，通过准静态单轴拉伸测试内侧副韧带和外侧副韧带的力学性能，比较重复拉伸对它们力学性质的影响。使用双光子显微镜二次谐波成像，量化内侧副韧带和外侧副韧带的胶原纤维结构。将重复拉伸后的内侧副韧带和外侧副韧带分别置于弹性蛋白酶溶液中孵育12 h，进行单轴拉伸实验，明确弹性蛋白处理对韧带力学性质的影响。
结果与结论：①准静态单轴拉伸测试显示，内侧副韧带的高张弹性模量高于外侧副韧带(P < 0.05)，两组韧带间的低张弹性模量比较差异无显著性意义(P > 0.05)；重复拉伸会显著降低内侧副韧带和外侧副韧带的低张弹性模量。②弹性蛋白酶溶液处理会显著降低内侧副韧带和外侧副韧带的低张弹性模量和高张弹性模量，并且外侧副韧带的高张弹性模量下降程度高于内侧副韧带；弹性蛋白酶溶液处理后，内侧副韧带的低张弹性模量和高张弹性模量均高于外侧副韧带(P < 0.05)。③双光子成像显示内侧副韧带的胶原纤维维持卷曲结构，其纤维波动度显著高于外侧副韧带。④结果表明，内侧副韧带较外侧副韧带具有更强的弹性性能，弹性蛋白酶处理对外侧副韧带的力学性质影响更大，这些力学结果可能与内侧副韧带更加卷曲的胶原纤维排列结构相关。
https://orcid.org/0009-0004-5332-0195 (徐洪璋) 


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 内侧副韧带, 外侧副韧带, 生物力学, 弹性蛋白, 准静态拉伸, 二次谐波

Abstract: BACKGROUND: As crucial stabilizers of the knee joint, the medial collateral ligament and lateral collateral ligament play essential roles in restricting valgus and varus movements, respectively. However, the mechanical differences between the medial collateral ligament and lateral collateral ligament, the microstructure characteristics, and the effect of elastin degradation on their mechanical properties remain poorly understood.
OBJECTIVE: To compare the mechanical differences between the medial collateral ligament and lateral collateral ligament, quantify the structural characteristics of the collagen fiber alignment, and investigate the impact of elastin degradation on the mechanical properties.
METHODS: The medial collateral ligament and lateral collateral ligament of the left side of adult pigs were obtained, frozen, and then thawed. The mechanical properties of the medial collateral ligament and lateral collateral ligament were tested using quasi-static uniaxial tensile, and the effect of repeated tensile on the mechanical properties was determined. The collagen fiber structure of the medial collateral ligament and lateral collateral ligament was quantified using two-photon microscopy and second-harmonic generation imaging. To evaluate the effect of elastin degradation on the mechanical properties, the medial collateral ligament and lateral collateral ligament under repeated stretching were incubated in an elastase solution for 12 hours before uniaxial stretching.
RESULTS AND CONCLUSION: (1) The medial collateral ligament exhibited a significantly higher high-tensile modulus of elasticity that the lateral collateral ligament (P < 0.05). There was no significant difference in low-tensile modulus of elasticity between the two groups of ligaments (P > 0.05). Repeated stretching significantly reduced the low-tensile modulus of both the medial collateral ligament and lateral collateral ligament. (2) Elastase treatment led to a significant decrease in both the low- and high-tensile elastic modulus of the medial collateral ligament and lateral collateral ligament, with the reduction of the high-tensile modulus in the lateral collateral ligament being more pronounced than the medial collateral ligament. Following elastase treatment, the low- and high-tensile moduli of the medial collateral ligament were significantly lower than those of the lateral collateral ligament (P < 0.05). (3) Two-photon imaging showed that the collagen fibers of the medial collateral ligament maintained a curled structure, and their fiber waviness was significantly higher than that of the lateral collateral ligament. These findings indicate that the medial collateral ligament exhibits greater elastic properties than the lateral collateral ligament, and elastase treatment has a more substantial impact on the mechanical properties of the lateral collateral ligament. This mechanical behavior might be attributed to the more coiled collagen fiber alignment in the medial collateral ligament.

Key words: medial collateral ligament, lateral collateral ligament, biomechanics, elastin, quasi-static tensile, second harmonics 

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