中国组织工程研究 ›› 2020, Vol. 24 ›› Issue (14): 2263-2270.doi: 10.3969/j.issn.2095-4344.2461

• 组织构建综述 tissue construction review • 上一篇    下一篇

外泌体和细胞因子促进牙髓血管生成的作用与调控机制

陈  婷1,2,李心竹1,2,徐稳安1,2   

  1. 1南方医科大学南方医院(第一临床医学院),广东省广州市  510515;2南方医科大学口腔医学院,广东省广州市  510515
  • 收稿日期:2019-06-29 修回日期:2019-07-02 接受日期:2019-08-09 出版日期:2020-05-18 发布日期:2020-03-16
  • 通讯作者: 徐稳安,博士,副教授,南方医科大学南方医院(第一临床医学院),广东省广州市 510515;南方医科大学口腔医学院,广东省广州市 510515
  • 作者简介:陈婷,女,1997年生,广东省梅州市人,汉族,南方医科大学在读。
  • 基金资助:
    国家自然科学基金(81700956);南方医科大学2018年省级大学生创新训练计划项目(201812121134);南方医科大学南方医院院长基金(2016B028)

Role of angiogenesis in dental pulp regeneration: exosomes and angiogenic factors#br#

Chen Ting1, 2, Li Xinzhu1, 2, Xu Wenan1, 2   

  1. 1Nanfang Hospital, Southern Medical University/The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, Guangdong Province, China; 2School of Stomatology, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • Received:2019-06-29 Revised:2019-07-02 Accepted:2019-08-09 Online:2020-05-18 Published:2020-03-16
  • Contact: Xu Wenan, MD, Associate professor, Nanfang Hospital, Southern Medical University/The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, Guangdong Province, China; School of Stomatology, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • About author:Chen Ting, Nanfang Hospital, Southern Medical University/The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, Guangdong Province, China; School of Stomatology, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • Supported by:
     the National Natural Science Foundation of China, No. 81700956; 2018 Provincial College Students Innovation Training Program of Southern Medical University, No. 201812121134; Dean’s Fund of Southern Hospital Affiliated to Southern Medical University, No. 2016B028

摘要:

文题释义:
牙髓再生:被定义为牙根结构、牙本质、牙髓牙本质复合体的更换,包括其血运、神经的重建,以弥补牙齿低血运和低感觉带来的使用寿损。牙髓再生治疗现已作为牙髓感染或坏死的一个新的治疗选择,组织工程技术促进牙髓再生,即将具有增殖分化潜能的干细胞和适宜的生物活性支架物质植入患牙牙根内,在一定生长因子的诱导下,产生新的牙本质牙髓复合体。
血管生成:是指从已有的毛细血管或毛细血管后静脉发展而形成新的血管,主要包括激活期血管基底膜降解;血管内皮细胞的激活、增殖、迁移;重建形成新的血管和血管网。血管形成是一个促血管形成因子和抑制因子协调作用的复杂过程,正常情况下二者处于平衡状态,一旦此平衡打破就会激活血管系统使血管生成过度,或抑制血管系统使血管退化。

背景:随着组织工程技术在口腔领域的应用,构建一种组织工程化的牙髓进行牙髓-牙本质复合体的再生治疗成为可能,而在牙髓再生过程中,形成有功能的血运系统是其必要条件。血管生成是指从已有的毛细血管中形成新的血管,对牙髓再生和维持牙髓内环境稳定具有重要意义。

目的:综述外泌体和促进血管生成的细胞因子在牙髓血管生成过程中作用机制的研究进展。

方法:应用计算机检索PubMed数据库和CNKI数据库,英文关键词为“tissue engineering,pulp regeneration,regenerative endodontics,angiogenesis,neovascularization,angiogenic,signal molecules,exosomes,factors,role,mechanism”,中文关键词为“牙髓再生,组织工程,血管生成,血管再生,信号分子,外泌体,因子,机制”,重点查阅从2017至2019年的相关文献并总结。 

结果与结论:组织工程中移植的牙髓组织能否存活取决于其能否快速形成有功能的血运系统。牙髓再生过程中血管生成是一个不可缺少的过程,许多文献研究了外泌体和多种促血管生成的细胞因子在促进牙髓血管生成中的作用,然而牙髓血管生成的调控机制还有待进一步研究。

ORCID: 0000-0003-3438-2310(陈婷)

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

关键词: 组织工程, 牙髓再生, 血管生成, 促进血管生成, 信号分子, 外泌体, 细胞因子, 作用机制

Abstract:

BACKGROUND: With the application of tissue engineering technology in the field of stomatology, it is possible to construct a tissue-engineered dental pulp for the regeneration of dentin-pulp complex. Formation of new blood supply system through angiogenesis is mandatory to dental pulp regeneration. Angiogenesis is defined as the formation of new blood vessels from preexisting capillaries, which has great significance in pulp regeneration and homeostasis. 

OBJECTIVE: To review the contribution of exosomes and angiogenic factors to angiogenesis in the dental pulp.

METHODS: A search of PubMed and CNKI was performed for relevant literature published from 2017 through 2019. The search terms were “tissue engineering, pulp regeneration, regenerative endodontics, angiogenesis, neovascularization, angiogenic, signal molecules, exosomes, factors, role, mechanism” in English and Chinese, respectively. 

RESULTS AND CONCLUSION: Many studies have indicated an intimate relationship between angiogenesis and dental pulp regeneration. The contribution of exosomes and angiogenic factors to angiogenesis of the dental pulp has been previously discussed. Angiogenesis is an indispensable process during dental pulp regeneration. The survival of transplanted pulp tissue is closely linked to the process of angiogenesis at sites of application. However, further investigations are warranted on the detailed regulatory mechanisms of exosomes and factors involved in initiation and progression of angiogenesis in pulp tissue.

Key words: tissue engineering, dental pulp regeneration, angiogenesis, pro-angiogenesis, exosomes, cytokines, mechanism of action

中图分类号: