中国组织工程研究

中国组织工程研究 ›› 2026, Vol. 30 ›› Issue (32): 8436-8442.doi: 10.12307/2026.458

• 水凝胶材料Hydrogel materials • 上一篇    下一篇

负载槲皮素-纳米银粒子光敏水凝胶应用于感染创口的抗菌性能

陈世超1，2，3，邓云艺1，2，3，赵任圣洁1，2，3，余  科1，2，3，李广文1，2，3   

  1. 1西南医科大学口腔医学院种植科，四川省泸州市   646000；2口颌面修复重建和再生泸州市重点实验室，四川省泸州市   646000；3西南医科大学口腔医学研究所，四川省泸州市   646000
  • 接受日期:2026-02-07 出版日期:2026-11-18 发布日期:2026-04-27
  • 通讯作者: 李广文，博士，副主任医师，西南医科大学口腔医学院种植科，四川省泸州市 646000；口颌面修复重建和再生泸州市重点实验室， 四川省泸州市 646000；西南医科大学口腔医学研究所，四川省泸州市 646000 余科，博士，副教授，西南医科大学口腔医学院种植科，四川省泸州市 646000；口颌面修复重建和再生泸州市重点实验室，四川省泸州市 646000；西南医科大学口腔医学研究所，四川省泸州市 646000
  • 作者简介:陈世超，男，2000年生，福建省莆田市人，汉族，硕士，主要从事水凝胶抗菌敷料研究。
  • 基金资助:
    四川省自然科学基金面上项目(2024NSFSC0680)，项目负责人：李广文；四川省卫生健康委员会科技项目(青年苗圃项目：24QNMP018)，项目负责人：李广文；西南医科大学附属口腔医院博士科研启动项目(2025BS03)，项目负责人：李广文

Antibacterial properties of photocrosslinkable hydrogel loaded with quercetin-silver nanoparticles for infected wounds

Chen Shichao1, 2, 3, Deng Yunyi1, 2, 3, Zhao Renshengjie1, 2, 3, Yu Ke1, 2, 3, Li Guangwen1, 2, 3   

  1. 1Department of Implantology, School of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China; 2Luzhou Key Laboratory of Oral and Maxillofacial Reconstruction and Regeneration, Luzhou 646000, Sichuan Province, China; 3Institute of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Accepted:2026-02-07 Online:2026-11-18 Published:2026-04-27
  • Contact: Li Guangwen, PhD, Associate chief physician, Department of Implantology, School of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Luzhou Key Laboratory of Oral and Maxillofacial Reconstruction and Regeneration, Luzhou 646000, Sichuan Province, China; Institute of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China Yu Ke, PhD, Associate professor, Department of Implantology, School of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Luzhou Key Laboratory of Oral and Maxillofacial Reconstruction and Regeneration, Luzhou 646000, Sichuan Province, China; Institute of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • About author:Chen Shichao, MS, Department of Implantology, School of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Luzhou Key Laboratory of Oral and Maxillofacial Reconstruction and Regeneration, Luzhou 646000, Sichuan Province, China; Institute of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Supported by:
    Sichuan Provincial Natural Science Foundation (General Program), No. 2024NSFSC0680 (to LGW); Sichuan Provincial Health Commission Science and Technology Project, No. 24QNMP018 (to LGW); Doctoral Research Start-up Project of Affiliated Stomatological Hospital of Southwest Medical University, No. 2025BS03 (to LGW) 

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摘要：

文题释义：
槲皮素-纳米银粒子：槲皮素是著名的抗氧化药物，能够清除体内的自由基，减少氧化应激对细胞的损伤。银纳米颗粒是研究最广泛的金属纳米颗粒抗菌剂，具有广谱抗菌性能、稳定性强、极少产生耐药菌株等特点。槲皮素能与银纳米颗粒的表面反应形成稳定的酚银复合物，即槲皮素-纳米银粒子，这种表面修饰可以有效防止颗粒间的聚集，从而增加纳米颗粒的分散性和稳定性，兼具有两者特性的同时可以降低银离子的释放，减少对细胞和组织的潜在毒性。
甲基丙烯酰化透明质酸水凝胶：是基于细胞外基质中的重要组成部分透明质酸的衍生物，继承了透明质酸的生物相容性，能够为细胞提供一个接近生理环境的生长基质，减少免疫反应和组织排斥，还可通过可见光进行快速光固化，形成稳定的三维网络结构的特性。 

背景：多项研究证实，槲皮素能够抑制炎症因子的释放、减轻创口局部炎症反应，为创口愈合创造有利条件，然而，槲皮素存在水溶性差、生物利用度低等问题，限制了它在创口治疗中的应用。
目的：探讨槲皮素-纳米银粒子/甲基丙烯酰化透明质酸复合水凝胶的抗菌性能。
方法：①通过水热还原法合成槲皮素-纳米银粒子(记为QuAgNPs)，将QuAgNPs按照不同的质量浓度(1，5，10，20，40，60，80 μg/mL)负载于甲基丙烯酰化透明质酸水凝胶前驱液中制备复合水凝胶，分别记为1，5，10，20，40，60，80 μg/mL QuAgNPs/HAMA水凝胶，同时制备单纯的甲基丙烯酰化透明质酸水凝胶(记为HAMA水凝胶)。将金黄色葡萄球菌分别与1，5，10，20，40，60，80 μg/mL QuAgNPs/HAMA水凝胶共培养，通过抑菌圈实验检测抗菌性能。检测60，80 μg/mL QuAgNPs/HAMA水凝胶的体外药物释放性能。根据抑菌圈实验结果，选择合适的QuAgNPs/HAMA水凝胶用于后续实验。②扫描电镜下观察HAMA水凝胶、60 μg/mL QuAgNPs/HAMA水凝胶的微观形貌。将金黄色葡萄球菌(或大肠杆菌)分别与QuAgNPs、HAMA水凝胶、60 μg/mL QuAgNPs/HAMA水凝胶共培养，以单独培养的细菌为空白对照，通过涂布平板抗菌实验检测抑菌率。将人脐静脉内皮细胞分别与QuAgNPs、HAMA水凝胶浸提液、60 μg/mL QuAgNPs/HAMA水凝胶浸提液共培养，以单独培养的细胞为空白对照，活/死细胞染色检测细胞活力。
结果与结论：①抑菌圈实验显示，60，80 μg/mL QuAgNPs/HAMA水凝胶的抗菌性能强于1，5，10，20，40 μg/mL QuAgNPs/HAMA水凝胶，60，80 μg/mL QuAgNPs/HAMA水凝胶的抗菌性能无差异。60，80 μg/mL QuAgNPs/HAMA水凝胶均具有良好的体外药物释放性能。因此，后续实验选择60 μg/mL QuAgNPs/HAMA水凝胶。②扫描电镜显示，HAMA水凝胶呈疏松多孔状结构，60 μg/mL QuAgNPs/HAMA水凝胶可见近似球形的QuAgNPs粒子散在分布于HAMA水凝胶表面。涂布平板抗菌实验显示，QuAgNPs、60 μg/mL QuAgNPs/HAMA水凝胶对金黄色葡萄球菌与大肠杆菌均有显著的抑制作用。活/死细胞染色显示，QuAgNPs、HAMA水凝胶、60 μg/mL QuAgNPs/HAMA水凝胶未对细胞活力造成影响，均具有良好的细胞相容性。③结果表明，QuAgNPs/HAMA复合水凝胶具有良好的抗菌性能。
https://orcid.org/0009-0008-6286-0459 (陈世超) 

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料；口腔生物材料；纳米材料；缓释材料；材料相容性；组织工程

关键词: 槲皮素, 纳米银粒子, 甲基丙烯酰化透明质酸, 水凝胶, 抗菌性能, 感染创口

Abstract: BACKGROUND: Numerous studies have confirmed that quercetin can inhibit the release of inflammatory factors and reduce local inflammatory responses in wounds, creating favorable conditions for wound healing. However, quercetin suffers from poor water solubility and low bioavailability, limiting its application in wound treatment.
OBJECTIVE: To investigate the antibacterial properties of quercetin-silver nanoparticles (QuAgNPs)/methacrylated hyaluronic acid (HAMA) composite hydrogels. 
METHODS: (1) QuAgNPs were synthesized by hydrothermal reduction. QuAgNPs were loaded into methacrylated hyaluronic acid hydrogel precursor solution at different concentrations (1, 5, 10, 20, 40, 60, and 80 μg/mL) to prepare composite hydrogels, denoted as 1, 5, 10, 20, 40, 60, and 80 μg/mL QuAgNPs/HAMA hydrogels. A pure HAMA hydrogel was also prepared. Staphylococcus aureus was co-cultured with 1, 5, 10, 20, 40, 60, and 80 μg/mL QuAgNPs/HAMA hydrogels, and antibacterial properties were detected by the inhibition zone assay. The in vitro drug release performance of 60 and 80 μg/mL QuAgNPs/HAMA hydrogels was also tested. Based on the inhibition zone assay results, a suitable QuAgNPs/HAMA hydrogel was selected for subsequent experiments. (2) The microstructure of HAMA hydrogel and 60 μg/mL QuAgNPs/HAMA hydrogel was observed under a scanning electron microscope. Staphylococcus aureus (or Escherichia coli) was co-cultured with QuAgNPs, HAMA hydrogel, and 60 μg/mL QuAgNPs/HAMA hydrogel, with bacteria cultured alone as a blank control. The antibacterial rate was detected by the plate coating antibacterial experiment. Human umbilical vein endothelial cells were co-cultured with QuAgNPs, HAMA hydrogel extract, and 60 μg/mL QuAgNPs/HAMA hydrogel extract, respectively, with cells cultured alone as a blank control. Cell viability was detected by live/dead cell staining. 
RESULTS AND CONCLUSION: (1) The inhibition zone experiment showed that the antibacterial performance of 60 and 80 μg/mL QuAgNPs/HAMA hydrogels was stronger than that of 1, 5, 10, 20, and 40 μg/mL QuAgNPs/HAMA hydrogels, and there was no difference in antibacterial performance between 60 and 80 μg/mL QuAgNPs/HAMA hydrogels. Both 60 and 80 μg/mL QuAgNPs/HAMA hydrogels exhibited good in vitro drug release performance. Therefore, 60 μg/mL QuAgNPs/HAMA hydrogel was selected for subsequent experiments. (2) Scanning electron microscopy showed that the HAMA hydrogel had a loose and porous structure, and approximately spherical QuAgNPs particles were scattered on the surface of the HAMA hydrogel in the 60 μg/mL QuAgNPs/HAMA hydrogel. The plate coating antibacterial experiment showed that QuAgNPs and 60 μg/mL QuAgNPs/HAMA hydrogel had significant inhibitory effects on both Staphylococcus aureus and Escherichia coli. Live/dead cell staining showed that QuAgNPs, HAMA hydrogel, and 60 μg/mL QuAgNPs/HAMA hydrogel did not affect cell viability and all had good cytocompatibility. (3) The results indicate that the QuAgNPs/HAMA composite hydrogel has good antibacterial properties. 

Key words: quercetin, silver nanoparticles, methacrylated hyaluronic acid, hydrogel, antibacterial property, infected wounds

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