中国组织工程研究

中国组织工程研究 ›› 2026, Vol. 30 ›› Issue (12): 2949-2956.doi: 10.12307/2026.655

• 骨组织构建 bone tissue construction •    下一篇

敲低Linc00052表达对成骨细胞增殖、迁移和凋亡的影响

齐  鲁1，王俊杰1，邓钦高2，王  星2   

  1. 1新疆医科大学第二附属医院口腔科，新疆维吾尔自治区乌鲁木齐市  830063；2新疆医科大学第一附属医院口腔修复科，新疆维吾尔自治区乌鲁木齐市  830054


  • 收稿日期:2025-02-20 接受日期:2025-07-28 出版日期:2026-04-28 发布日期:2025-09-28
  • 通讯作者: 王星，副主任医师，新疆医科大学第一附属医院口腔修复科，新疆维吾尔自治区乌鲁木齐市 830054
  • 作者简介:齐鲁，男，山东省淄博市人，汉族，硕士，副教授，副主任医师，主要从事口腔临床医学研究。
  • 基金资助:
    新疆维吾尔自治区自然科学基金面上项目(2023D01C116)，项目负责人：齐鲁

Knockdown of Linc00052 influences osteoblast proliferation, migration and apoptosis

Qi Lu1, Wang Junjie1, Deng Qingao2, Wang Xing2   

  1. 1Department of Stomatology, Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830063, Xinjiang Uygur Autonomous Region, China; 2Department of Stomatology and Prosthodontics, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Received:2025-02-20 Accepted:2025-07-28 Online:2026-04-28 Published:2025-09-28
  • Contact: Wang Xing, Associate chief physician, Department of Stomatology and Prosthodontics, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • About author:Qi Lu, MS, Associate professor, Associate chief physician, Department of Stomatology, Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830063, Xinjiang Uygur Autonomous Region, China
  • Supported by:
    the Natural Science Foundation of Xinjiang Uygur Autonomous Region (General Program), No. 2023D01C116 (to QL)

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摘要：


文题释义：
根尖周炎：是指发生在牙齿根尖部及其周围组织的炎症性疾病。根尖周炎主要是由牙齿根尖部感染和炎症引起，通常与未治疗的牙髓炎或根管感染有关，主要表现为根尖周组织的慢性炎症和牙槽骨缺损。
长链非编码RNA：是一类长度大于200个核苷酸的非编码RNA分子，具有多种生物学功能，在细胞增殖、分化、凋亡及基因调控、炎症反应、成骨代谢等方面发挥着重要作用。

背景：Linc00052与多种疾病的发生和发展密切相关，包括癌症和炎症性疾病。研究发现，Linc00052靶向miR-145发挥人关节软骨细胞损伤的保护作用。
目的：观察敲低Linc00052对成骨细胞增殖、迁移和凋亡的影响
方法：①收集30例人根尖周炎骨组织与30例人健康根尖周骨组织，RT-qPCR检测Linc00052、miR-145表达。②将第3代CP-H111成骨细胞分2组培养：实验组转染慢病毒敲低Linc00052表达，对照组转染空载慢病毒阴性对照，转染72 h后，RT-qPCR检测Linc00052、miR-145表达，Western blot检测肿瘤坏死因子α与转化生长因子β/SMAD2/SMAD3信号通路蛋白表达，CCK-8实验检测细胞增殖，划痕实验、Transwell小室实验检测细胞迁移，流式细胞术检测细胞凋亡。
结果与结论：①根尖周炎根尖周骨组织中Linc00052表达高于健康根尖周骨组织(P < 0.05)，miR-145表达低于健康根尖周骨组织(P < 0.05)；②实验组细胞内Linc00052表达、细胞凋亡与肿瘤坏死因子α蛋白表达低于对照组(P < 0.05)，miR-145表达与转化生长因子β1、p-SMAD2、p-SMAD3蛋白表达高于对照组，细胞迁移能力强于对照组；③结果表明，敲低Linc00052可通过负调控miR-145表达降低肿瘤坏死因子α蛋白表达、激活转化生长因子β/SMAD2/SMAD3信号通路，促进成骨细胞的增殖、迁移并抑制细胞凋亡。
https://orcid.org/0000-0001-9488-297X(齐鲁)

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: Linc00052, 成骨细胞, 肿瘤坏死因子α, 转化生长因子β/SMAD2/SMAD3信号通路, 根尖周炎骨缺损

Abstract: BACKGROUND: Linc00052 is associated with the occurrence and development of various diseases, including cancer and inflammatory diseases. It was found that Linc00052 targets miR-145 to exert a protective effect against human articular chondrocyte injury.
OBJECTIVE: To observe the roles of Linc00052 knockdown in the proliferation, migration and apoptosis of osteoblasts.
METHODS: (1) Human periapical bone tissues were collected from 30 patients with periapical inflammation and 30 healthy controls. Expression of Linc00052 and miR-145 was detected by RT-qPCR. (2) Passage 3 CP-H111 osteoblasts were cultured in two groups: the experimental group was transfected with lentivirus to knock down the expression of Linc00052, and the control group was transfected with an empty lentiviral negative control. At 72 hours after the transfection, RT-qPCR was used to detect the expression of Linc00052 and miR-145; western blot was used to detect the protein expression of tumor necrosis factor α and transforming growth factor β/SMAD2/SMAD3 signaling pathway proteins; cell counting kit-8 assay was used to detect cell proliferation; cell scratch assay and Transwell assay were conducted to detect cell migration; and flow cytometry was used to detect cell apoptosis.
RESULTS AND CONCLUSION: (1) The expression of Linc00052 in periapical bone tissue of periapical periodontitis was significantly higher than that in normal periapical bone tissue (P < 0.05). (2) The expression of Linc00052, apoptosis level and protein expression of tumor necrosis factor α in the experimental group were lower than those of the control group (P < 0.05), while the protein expression of miR-145, transforming growth factor β1, p-SMAD2, and p-SMAD3 was higher than that in the control group, and the cell migration ability was stronger than that of the control group. To conclude, knockdown of Linc00052 expression can promote osteoblast proliferation and migration and inhibit cell apoptosis by reducing the protein expression of tumor necrosis factor α and activating the transforming growth factor β/SMAD2/SMAD3 signaling pathway.

Key words: Linc00052, osteoblast, tumor necrosis factor α, transforming growth factor β/SMAD2/SMAD3 pathway signaling pathway, periapical bone loss

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