中国组织工程研究

中国组织工程研究 ›› 2025, Vol. 29 ›› Issue (26): 5536-5542.doi: 10.12307/2025.769

• 血管组织构建 vascular tissue construction • 上一篇    下一篇

高糖对hCMEC/D3人脑微血管内皮细胞血脑屏障紧密连接的影响

杨洪涛1，2，许永劼1，周勇君1，2，王  双2，黄昶煜东1，朱丽英2，3，潘  卫1，2   

  1. 贵州医科大学附属医院，1贵州省产前诊断中心，3临床检验中心，贵州省贵阳市  550004；2贵州医科大学医学检验学院，贵州省贵阳市  550004


  • 收稿日期:2024-08-27 接受日期:2024-10-16 出版日期:2025-09-18 发布日期:2025-02-21
  • 通讯作者: 潘卫，博士，教授，贵州医科大学附属医院贵州省产前诊断中心，贵州省贵阳市 550004；贵州医科大学医学检验学院，贵州省贵阳市 550004 共同通讯作者：朱丽英，博士，副教授，贵州医科大学医学检验学院，贵州省贵阳市 550004；贵州医科大学附属医院临床检验中心，贵州省贵阳市 550004
  • 作者简介:杨洪涛，男，1999年生，贵州医科大学在读硕士，主要从事糖尿病并发症发病机制研究。
  • 基金资助:
    国家自然科学基金地区科学基金项目(82260165)，项目负责人：潘卫；国家自然科学基金青年科学基金项目(82300920)，项目负责人：许永劼；贵州省科技计划项目(黔科合基础-ZK[2024]一般199)，项目负责人：许永劼；贵州省卫生健康委科学技术基金项目(gzwkj2024-081)，项目负责人：许永劼；贵州医科大学附属医院国家自然科学基金地区基金培育计划(gyfyhsfc-2022-37)，项目负责人：朱丽英

Effect of high glucose on blood-brain barrier tight junctions in hCMEC/D3 human brain microvascular endothelial cells

Yang Hongtao1, 2, Xu Yongjie1, Zhou Yongjun1, 2, Wang Shuang2, Huang Changyudong1, Zhu Liying2, 3, Pan Wei1, 2   

  1. 1Guizhou Prenatal Diagnosis Center, 3Clinical Laboratory Center, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China; 2School of Medical Laboratory Science, Guizhou Medical University, Guiyang 550004, Guizhou Province, China  

  • Received:2024-08-27 Accepted:2024-10-16 Online:2025-09-18 Published:2025-02-21
  • Contact: Pan Wei, PhD, Professor, Guizhou Prenatal Diagnosis Center, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China; School of Medical Laboratory Science, Guizhou Medical University, Guiyang 550004, Guizhou Province, China Co-corresponding author: Zhu Liying, PhD, Associate professor, School of Medical Laboratory Science, Guizhou Medical University, Guiyang 550004, Guizhou Province, China; Clinical Laboratory Center, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China
  • About author:Yang Hongtao, Master candidate, Guizhou Prenatal Diagnosis Center, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China; School of Medical Laboratory Science, Guizhou Medical University, Guiyang 550004, Guizhou Province, China
  • Supported by:
    National Natural Science Foundation of China (Regional Science Fund Project), No. 82260165 (to PW); National Natural Science Foundation of China (Youth Science Fund Project), No. 82300920 (to XYJ); Guizhou Science and Technology Plan Project, No. ZK[2024]199 (to XYJ); Guizhou Provincial Health Commission Science and Technology Fund Project, No. gzwkj2024-081 (to XYJ); National Natural Science Foundation of Regional Cultivation Program of Affiliated Hospital of Guizhou Medical University, No. gyfyhsfc-2022-37 (to ZLY)

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摘要：


文题释义：
细胞紧密连接：是一种在细胞间形成的特殊结构，主要存在于上皮细胞(如皮肤和消化道内壁)和内皮细胞(血管内壁)之间的连接部位。这些连接像“锁链”一样将相邻的细胞紧密地连接在一起，形成一道有效的屏障，防止物质随意通过细胞间的空隙。 
组蛋白去乙酰化酶4：是一类依赖锌的去乙酰化酶，属于第Ⅱa类组蛋白去乙酰化酶，具有去乙酰化酶的活性。近年来的研究发现，组蛋白去乙酰化酶4在大脑中表达丰富，可参与基因的转录调控、细胞凋亡、代谢等诸多生物进程。

背景：血脑屏障是保护中枢神经系统的重要结构，研究高糖对血脑屏障的影响对预防高糖导致的中枢神经系统损伤有重要意义。
目的：探讨高糖对hCMEC/D3人脑微血管内皮细胞血脑屏障功能的潜在影响。
方法：hCMEC/D3细胞分别以常规糖培养基(葡萄糖浓度25 mmol/L)和高糖培养基(葡萄糖浓度55 mmol/L)培养，光学显微镜观察细胞形态，CCK-8法检测细胞活力变化。采用Transwell小室将hCMEC/D3细胞系建立单层细胞血脑屏障模型，每日监测细胞跨膜电阻值变化，通过苯酚红透过率检测单层细胞的通透性，流式细胞术检测细胞凋亡率，Western blot检测细胞中Bcl-2、Bax、Caspase-3、ZO-1、Occludin、Claudin-1、组蛋白去乙酰化酶蛋白4的表达，ELISA检测细胞上清液中组蛋白去乙酰化酶水平，免疫荧光检测细胞中组蛋白去乙酰化酶4的表达。
结果与结论：①高糖组细胞活力显著低于对照组(P < 0.000 1)；②对照组细胞生长状态良好，交织成致密网状，突触间相互连接；高糖组细胞生长受抑，细胞间突触连接减少；③与对照组相比，高糖组跨膜电阻值降低(P < 0.05)，单层细胞膜苯酚红通透性增加(P < 0.05)，细胞凋亡率升高(P < 0.01)，Bax蛋白表达增加(P < 0.000 1)，Caspase-3蛋白表达无明显变化(P > 0.05)，Bcl-2、ZO-1、Occludin、Claudin-1、组蛋白去乙酰化酶4蛋白表达降低(P < 0.01，P < 0.001，P < 0.01，P < 0.000 1，P < 0.01)，组蛋白去乙酰化酶4荧光表达降低(P < 0.001)；④高糖组细胞上清液中组蛋白去乙酰化酶4水平低于对照组(P < 0.05)。结果表明，高糖诱导hCEMCE/D3细胞凋亡增加，通透性增强，其机制可能与组蛋白去乙酰化酶4表达水平降低有关。
https://orcid.org/0009-0006-1957-1753(杨洪涛)

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 高糖, 血脑屏障, 紧密连接, 通透性, 细胞凋亡, 组蛋白去乙酰化酶4, 工程化组织构建

Abstract: BACKGROUND: The blood-brain barrier is an important structure that protects the central nervous system, and the study of the effects of high glucose on the blood-brain barrier is important for the prevention of high glucose-induced damage to the central nervous system. 
OBJECTIVE: To investigate the potential effect of high glucose on the blood-brain barrier function of hCMEC/D3 human brain microvascular endothelial cells.
METHODS: hCMEC/D3 cells were cultured in regular sugar medium (glucose concentration of 25 mmol/L) and high-sugar medium (glucose concentration of 55 mmol/L). The morphology of cells in each group was observed by light microscopy. CCK-8 assay was used to detect changes in cell viability. A monolayer blood-brain barrier model was established using hCMEC/D3 cell line with Transwell chamber device. Changes in cell transmembrane resistance were monitored daily. The permeability of cell monolayers was detected by phenol red permeability. Flow cytometry was used to detect the apoptosis rate of the cells. Western blot assay was used to detect the expression of Bcl-2, Bax, Caspase-3, ZO-1, Occludin, Claudin-1, and histone deacetylase 4. The levels of histone deacetylase in cell supernatant were detected by ELISA. The expression of histone deacetylase 4 in cells was detected by immunofluorescence. 
RESULTS AND CONCLUSION: (1) The cell viability of high sugar group was significantly lower than that of control group (P < 0.000 1). (2) The cells of the control group were in a good growth state, interwoven into a dense mesh, with interconnections between synapses. The cell growth of high glucose group was suppressed, and the connection of inter-cellular synapses was reduced. (3) Compared with the control group, the transmembrane resistance value of the high glucose group was reduced (P < 0.05); phenol-red permeability of the monolayer cell membrane was increased (P < 0.05); cell apoptosis rate was increased (P < 0.01); the expression of Bax protein was increased (P < 0.000 1); the expression of Caspase-3 protein had no significant change (P > 0.05); the expression of Bcl-2, ZO-1, Occludin, Claudin-1, and histone deacetylase 4 proteins was decreased (P < 0.01, P < 0.001, P < 0.01, P < 0.000 1, P < 0.01); the fluorescence expression of histone deacetylase 4 was decreased (P < 0.001) in the high glucose group. (4) The level of histone deacetylase 4 in the cell supernatant of the high glucose group was lower than that of the control group (P < 0.05). The results show that high glucose induces the increased apoptosis and enhances permeability of hCEMCE/D3 cells, and its mechanism may be related to the decreased expression level of histone deacetylase 4.

Key words: high glucose, blood-brain barrier, tight junction, permeability, apoptosis, histone deacetylase 4, engineered tissue construction

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