中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (46): 8561-8565.doi: 10.3969/j.issn.2095-4344.2012.46.003

• 骨组织构建 bone tissue construction • 上一篇    下一篇

咖啡酸对破骨细胞形成及组织蛋白酶K基因表达的抑制

唐全勇，贾志宇，赵云转，陈 彪，张 睿，杨 威，张英怀   

  1. 河北医科大学第二医院口腔颌面外科，河北省石家庄市 050000
  • 收稿日期:2012-03-05 修回日期:2012-04-18 出版日期:2012-11-11 发布日期:2013-03-16
  • 作者简介:唐全勇☆，男，1954年生，河北省顺平县人，汉族，2005年日本九州大学毕业，博士，主任医师，教授，主要从事破骨细胞与骨代谢方面研究。 tqy723@yahoo.com.cn

Caffeic acid inhibits osteoclast formation and expression of cathepsin K gene

Tang Quan-yong, Jia Zhi-yu, Zhao Yun-zhuan, Chen Biao, Zhang Rui, Yang Wei, Zhang Ying-huai   

  1. Department of Oral and Maxillofacial Surgery, the 2nd Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China
  • Received:2012-03-05 Revised:2012-04-18 Online:2012-11-11 Published:2013-03-16
  • About author:Tang Quan-yong☆, Doctor, Chief physician, Professor, Department of Oral and Maxillofacial Surgery, the 2nd Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China tqy723@yahoo.com.cn

PDF

527

被引次数

15

摘要：

背景：咖啡酸对破骨细胞形成分化方面影响的研究很少，对破骨细胞性骨吸收机制的研究更少。
目的：观察咖啡酸对破骨细胞形成分化及组织蛋白酶K基因表达的影响，分析甘薯提取物咖啡酸对破骨细胞性骨吸收的抑制机制。
方法：采用3种不同细胞培养系，观察咖啡酸对破骨细胞形成分化的影响，同时利用反转录-聚合酶链反应技术观察咖啡酸对组织蛋白酶K基因表达的抑制作用。
结果与结论：咖啡酸在3种不同细胞培养系中均显示出直接抑制破骨细胞形成和分化的作用，根据半定量反转录-聚合酶链反应基因表达分析，咖啡酸对RANKL受体RANK mRNA的表达完全无影响，但显著抑制了组织蛋白酶K的表达。提示甘薯提取物有效成分咖啡酸不仅能直接抑制破骨细胞的形成及分化，而且可显著抑制组织蛋白酶K的表达，其可能是甘薯提取物抑制破骨细胞性骨吸收的作用机制。

关键词: 咖啡酸, 组织蛋白酶K, 反转录-聚合酶链反应, 破骨细胞, 细胞培养

Abstract:

BACKGROUND: To date, there are few researches on the effect of caffeic acid on the formation and differentiation of osteoclasts, and fewer researches on the mechanism of osteoclastic bone resorption.
OBJECTIVE: To explore the mechanism of sweet potato extract caffeic acid inhibiting osteoclastic bone resorption by studying the effect of caffeic acid on the formation and differentiation of osteoclast and on the expression of cathepsin K gene.
METHODS: Caffeic acid effects on formation and differentiation of osteoclasts in three osteoclast cell lines were observed and the inhibition effect of caffeic acid on the expression of cathepsin K gene was detected by reverse transcription-PCR.
RESULTS AND CONCLUSION: Caffeic acid could directly inhibit the formation and differentiation of osteoclasts in three osteoclast cell lines. Caffeic acid had no influences on the expression of receptor activator of nuclear factor-kappa B ligand and receptor activator of nuclear factor-kappa B mRNA, but could markedly inhibit the expression of cathepsin K gene based on the semi-quantitative gene analysis of reverse transcription-PCR. Caffeic acid, an effective component of sweet potato extract, cannot only directly inhibit the formation and differentiation of osteoclast, but also markedly inhibit the expression of cathepsin K gene, which seems to be the mechanism of sweet potato extract inhibiting osteoclastic bone resorption.

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