中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (42): 7849-7852.doi: 10.3969/j.issn.2095-4344.2012.42.011

• 口腔组织构建 oral tissue construction • 上一篇    下一篇

周期性应力刺激对成牙骨质样细胞增殖的影响

任嫒姝1，白 丁2，黄 兰1，付 钢3   

  1. 重庆医科大学附属口腔医院，1正畸科，3 VIP中心，重庆市 400015；2 四川大学华西口腔医学院正畸科，四川大学口腔疾病国家重点实验室，四川省成都市 610041
  • 收稿日期:2012-04-02 修回日期:2012-06-04 出版日期:2012-10-14 发布日期:2013-04-08
  • 通讯作者: 付钢，博士，副主任医师，重庆医科大学附属口腔医院VIP中心，重庆市 400015
  • 作者简介:任嫒姝☆，女，1979年生，四川省蓬溪县人，汉族，2007年四川大学毕业，博士，副主任医师，主要从事口腔正畸生物力学的研究。 rasras@163.com

Effects of mechanical stress on proliferation of cementoblast-like cells

Ren Ai-shu1, Bai Ding2, Huang Lan1, Fu Gang3   

  1. 1Department of Orthodontics, 3VIP Center, Affiliated Stomatological Hospital, Chongqing Medical University, Chongqing 400015, China; 2Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2012-04-02 Revised:2012-06-04 Online:2012-10-14 Published:2013-04-08
  • Contact: Fu Gang, Doctor, Associate chief physician, VIP Center, Affiliated Stomatological Hospital, Chongqing Medical University, Chongqing 400015, China
  • About author:Ren Ai-shu, Doctor, Associate chief physician, Department of Orthodontics, Affiliated Stomatological Hospital, Chongqing Medical University, Chongqing 400015, China rasras@163.com

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758

被引次数

1

摘要：

背景：成牙骨质细胞在牙根形成、牙周病牙骨质的再生以及正畸牙根吸收的修复中起着重要的作用，其在体外培养状态下对力学信号反应特性的变化，在国内外少见报道。
目的：分析正畸牙移动张力侧及压力侧两种不同受力形式与牙骨质改建的关系。
方法：采用四点弯曲细胞力学加载仪，对体外培养的成牙骨质样细胞OCCM30分别施加2 000 με张、压应力，应用流式细胞术检测应力刺激3，6，12，24 h后OCCM30细胞周期及增殖指数的变化。
结果与结论：2 000 με张、压应力加载3，6 h后，成牙骨质样细胞OCCM30在S期的细胞百分率明显减小，细胞增殖活性降低；随着加力时间的延长，S期细胞百分率回升，加载24 h后S期细胞百分率与对照组无明显差异，细胞增殖活性恢复。张、压应力对增殖活性的影响趋势一致，相互之间差别无显著性意义。

关键词: 应力, 成牙骨质样细胞, 增殖, 正畸, 牙, 组织改建

Abstract:

BACKGROUND: Cementoblasts play an important role during root formation, cementum regeneration of periodontitis and repair of orthodontic root resorption. The influence of mechanical stimulation on cementoblasts in vitro is rarely reported.
OBJECTIVE: To analyze the relationship between different stress patterns at tension and compression sides during orthodontic tooth movement and cementum remodeling.
METHODS: 2000 με tensile and compressive stress was exerted, separately, on cementoblast-like cells OCCM30 by four-point bending system. The flow cytometry was used to examine the cell cycle and proliferation activity of OCCM30 after 3-, 6-, 12-, 24-hour loading.
RESULTS AND CONCLUSION: 2000 με tensile and compressive stress both depressed proliferation activity of OCCM30 through decreasing S-phase fraction after 3 and 6 hours loading. The S-phase fraction rose again with time prolonged. After 24 hours loading, the S-phase fraction and proliferation activity resumed to the level of controls. There was no significant difference between tensile and compressive stress in the influence on proliferation activity.

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