中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (40): 7486-7490.doi: 10.3969/j.issn.2095-4344.2012.40.015

• 移植与免疫 transplantation and Immunology • 上一篇    下一篇

应用环磷酰胺构建C57BL/6J小鼠免疫抑制模型

杨宪勇   

  1. 泰安市中心医院，山东省泰安市 271000
  • 收稿日期:2012-06-08 修回日期:2012-06-18 出版日期:2012-09-30 发布日期:2012-09-30
  • 作者简介:杨宪勇★，男，1973年生，山东省肥城市人，硕士，主管技师，主要从事医学免疫学工作。 yang_xianyong@yahoo.com.cn

Establishing a C57BL/6J mouse immunosuppressive model induced by cyclophosphamide

Yang Xian-yong   

  1. Taian City Central Hospital, Taian 271000, Shandong Province, China
  • Received:2012-06-08 Revised:2012-06-18 Online:2012-09-30 Published:2012-09-30
  • About author:Yang Xian-yong★, Master, Technician- in-charge, Taian City Central Hospital, Taian 271000, Shandong Province, China yang_xianyong@yahoo.com.cn

PDF

870

被引次数

39

摘要：

背景：在免疫增强剂的研究中，常用到免疫抑制模型，如何建立免疫抑制模型成为免疫增强剂作用评价的关键。
目的：应用环磷酰胺构建C57BL/6J小鼠免疫抑制模型。
方法：将小鼠随机分为正常对照组、环磷酰胺50 mg/kg 5 d组，环磷酰胺80 mg/kg 3 d组，环磷酰胺80 mg/kg 5 d组，环磷酰胺100 mg/kg 5 d组和环磷酰胺100 mg/kg隔天给药组。正常对照组小鼠腹腔注射生理盐水0.1 mL，连续5 d。环磷酰胺50 mg/kg 5 d组，环磷酰胺80 mg/kg 3 d组，环磷酰胺80 mg/kg 5 d组和环磷酰胺100 mg/kg 5 d组小鼠分别以环磷酰胺50，80，80，100 mg/kg腹腔注射连续5，3，5，5 d。环磷酰胺100 mg/kg隔天给药组小鼠腹腔注射100 mg/kg环磷酰胺，隔天1次，共注射3次。
结果与结论：与正常对照组比较，腹腔注射环磷酰胺可导致小鼠外周血中CD3+T，CD3+CD4+T及CD3+CD8+T细胞明显下降(P < 0. 05)；谷丙转氨酶(除环磷酰胺50 mg/kg 5 d、80 mg/kg 3 d组)、谷草转氨酶、尿素显著升高(P < 0.05)，其中环磷酰胺80 mg/kg 5 d组、环磷酰胺100 mg/kg 5 d组和环磷酰胺100 mg/kg隔天给药组对肝肾功能的影响更为明显。提示腹腔注射环磷酰胺可建立CD3+T，CD3+CD4+T及CD3+CD8+T细胞明显下降的免疫抑制模型，其中以环磷酰胺50 mg/kg 5 d和80 mg/kg 3 d方式对肝肾功能的损伤较小。

关键词: 免疫抑制模型, C57BL/6J小鼠, CD3+T淋巴细胞, CD3+CD4+T淋巴细胞, CD3+CD8+T淋巴细胞, 环磷酰胺

Abstract:

BACKGROUND: The immunosuppressive models are commonly used in the research of immunostimulants, but how to establish the immunosuppressive models is the key for the evaluation of immunostimulants.
OBJECTIVE: To establish C57BL/6J mice immunosuppressive model induced by cyclophosphamide.
METHODS: Normal C57BL/6J mice were randomly divided into six groups (n=4). Group 1 was normal control group, group 2 was injected with 50 mg/kg cyclophosphamide for 5 days, group 3 was injected with 80 mg/kg cyclophosphamide for 3 days, group 4 was injected with 80 mg/kg cyclophosphamide for 5 days, group 5 was injected with 100 mg/kg cyclophosphamide for 5 days, and group 6 was injected with 100 mg/kg cyclophosphamide every 2 days. Mice in group 1 were intraperitoneal injected with 0.1 mL normal saline and lasted for 5 days. Mice in group 2, 3, 4, 5 were intraperitoneal injected with 50, 80, 80 and 100 mg/kg cyclophosphamide, respectively, and lasted for 5, 3, 5 and 5 days, respectively. Mice in group 6 were intraperitoneal injected with 100 mg/kg cyclophosphamide every two days and total injected for three times.
RESULTS AND CONCLUSION: Compared with group 1, the CD3+T, CD3+CD4+T and CD3+CD8+T levels in the peripheral blood in the other five groups were significantly decreased (P < 0. 05); the contents of alanine aminotransferase (except for group 2 and group 3), aspartate aminotransferase and blood urea nitrogen in the other five groups were significantly increased (P < 0. 05), especially in group 4, 5 and 6. Intraperitoneal injection of cyclophosphamide can establish the immunosuppressive model with decreased level of CD3+T, CD3+CD4+T and CD3+CD8+T, and intraperitoneal injection of 50 mg/kg and 80 mg/kg cyclophosphamide for 5 and 3 days has less kidney and liver damage.

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