中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (16): 2865-2868.doi: 10.3969/j.issn.1673-8225.2012.16.004

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

羟基丁酸与羟基辛酸共聚物一体化骨软骨组织工程支架的制备及性能*★◆

郭霄飞1，张永红2，杜美丽1，吕利华1，陈学英3，赵良启1   

  1. 1山西大学生物技术研究所化学生物学与分子工程教育部重点实验室，山西省太原市  030006；2山西医科大学第二医院骨科，山西省太原市  030001；3山西省医用组织库，山西省太原市  030006
  • 收稿日期:2011-11-15 修回日期:2012-02-23 出版日期:2012-04-15 发布日期:2012-04-15
  • 通讯作者: 赵良启，博士生导师，教授，山西大学化学生物学与分子工程教育部重点实验室，山西省太原市 030006 liangqi@ sxu.edu.cn
  • 作者简介:郭霄飞★，男，1985年生，山西省临汾市人，汉族，山西大学在读硕士，主要从事微生物生化工程及生物医用材料研究。 sx_xiaofei@ 126.com
  • 基金资助:

    国家自然科学基金主任基金项目(31040030)，项目名称：新型多聚羟基烷酸软骨组织工程载体的研究。

Preparation and characterization of an integrative poly (hydroxybutyrate-co-hydroxyoctanoate) osteochondral tissue-engineering scaffold

Guo Xiao-fei1, Zhang Yong-hong2, Du Mei-li1, Lü Li-hua1, Chen Xue-ying3, Zhao Liang-qi1   

  1. 1Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan  03006, Shanxi Province, China; 2Department of Orthopedics, Second Hospital of Shanxi Medical University, Taiyuan  03001, Shanxi Province, China; 3Shanxi Tissue Bank, Taiyuan  03001, Shanxi Province, China
  • Received:2011-11-15 Revised:2012-02-23 Online:2012-04-15 Published:2012-04-15
  • Contact: author: Zhao Liang-qi, Doctoral supervisor, Professor, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 03006, Shanxi Province, China liangqi@ sxu.edu.cn
  • About author:Guo Xiao-fei★, Studying for master’s degree, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 03006, Shanxi Province, China sx_xiaofei@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 31040030*

PDF

535

摘要：

背景：单层支架难以满足关节软骨损伤修复的要求，现提出骨软骨共同修复的一体化支架，以弥补了单一支架的部分缺陷。
目的：以羟基丁酸与羟基辛酸共聚物为基础材料，羟基磷灰石等为复合材料研制一体化骨软骨组织工程支架，测试该支架的物理特性和细胞黏附性。
方法：采用溶剂浇铸/颗粒沥滤法，以支架孔径、孔隙率、力学强度和细胞黏附生长率为检测指标，以羟基丁酸与羟基辛酸共聚物为连续相，通过改变致孔剂NaCl粒径和羟基磷灰石材料配比制备不同形态结构、力学强度和生物学功能的三层一体化骨软骨组织工程支架。
结果与结论：致孔剂与支架材料的最佳质量配比分别为软骨层4.5/1，过渡层2.5/1，硬骨层3.5/1。扫描电镜观察显示支架的三层结构明显不同且紧密结合，其软骨层、过渡层、硬骨层的孔径分别为150~250 μm，≤60 μm，150~450 μm；孔隙率检测结果依次为84%，60%，75%；力学强度测定依次为2.93，6.43，4.30 MPa；支架对骨髓间充质干细胞无毒性，细胞黏附与生长状态良好。结果表明该一体化骨软骨组织工程支架具有仿生学特性，符合骨软骨组织工程支架的基本条件。
关键词：羟基丁酸；羟基辛酸；共聚物；一体化支架；关节软骨缺损；骨软骨组织工程
缩略语注释：PHBHO：poly(hydroxybutyrate-co-hydroxyoctanoate)，羟基丁酸与羟基辛酸共聚体；HA：hydroxyapatite，羟基磷灰石
doi:10.3969/j.issn.1673-8225.2012.16.004

关键词: 羟基丁酸, 羟基辛酸, 共聚物, 一体化支架, 关节软骨缺损, 骨软骨组织工程

Abstract:

BACKGROUND: Single-layer scaffold, however, is difficult to meet the requirements of articular cartilage injury and repair. The integrative scaffold makes up partial defects of a single-layer scaffold.
OBJECTIVE: To prepare an integrative scaffold made of poly (hydroxybutyrate-co-hydroxyoctanoate)-hydroxyapatite to repair articular cartilage and subchondral bone defects.
METHODS: The scaffold was prepared by solvent casting/particle leaching method, and its aperture, porosity, mechanical strength and cell adhesion growth were tested.
RESULTS AND CONCLUSION: The optimal ratio of porogenic agent to scaffold material was 4.5/1 for the cartilage layer, 2.5/1 for the middle layer, and 3.5/1 for the bone layer. The porosity and pore size of the scaffold were 75% and 150-450 μm in the bone layer, 84% and 150-250 μm in the cartilage layer, 60% and ≤60 μm in the middle layer, respectively. The layers linked to be a whole one closely. The mechanical strength was 4.3, 6.43, and 2.93 MPa from the bone layer to the cartilage layer. The scaffold displayed good affinity to the cells. The technique of integrative scaffold made of poly (hydroxybutyrate-co-hydroxyoctanoate)-hydroxyapatite w as developed. The scaffold may be a competent candidate in application in articular cartilage damage repair.

中图分类号: