中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (28): 5269-5272.doi: 10.3969/j.issn.1673-8225.2011.28.035

• 肌肉肌腱韧带组织构建 tissue construction of the muscle, tendon and ligament • 上一篇    下一篇

骨骼肌细胞αB-晶状体蛋白基因在大强度离心运动后的表达

张立立，杨惠玲，翟凤鸣，张瑞存，李壮志   

  1. 石家庄学院体育系，河北省石家庄市  050035
  • 收稿日期:2011-02-09 修回日期:2011-05-13 出版日期:2011-07-09 发布日期:2011-07-09
  • 作者简介:张立立★，女，1974年生，河北省石家庄市人，汉族，2004年河北师范大学体育学院毕业，硕士，讲师，主要从事运动生理学的教学与科研工作。 zhang197li@126.com

Expression of alpha B-crystallin mRNA of skeletal muscle cells after high-intensity eccentric exercise

Zhang Li-li, Yang Hui-ling, Zhai Feng-ming, Zhang Rui-cun, Li Zhuang-zhi   

  1. Department of Physical Education, Shijiazhuang University, Shijiazhuang  050035, Hebei Province, China
  • Received:2011-02-09 Revised:2011-05-13 Online:2011-07-09 Published:2011-07-09
  • About author:Zhang Li-li★, Master, Lecturer, Department of Physical Education, Shijiazhuang University, Shijiazhuang 050035, Hebei Province, China zhang197li@126.com

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摘要：

背景：骨骼肌含有被称为“分子伴侣”的小热休克蛋白αB-晶状体蛋白，有可能在肌肉运动中具有重要的生理功能。
目的：观察离心运动后骨骼肌细胞αB-晶状体蛋白基因表达。
方法：将成年雄性Wistar大鼠随机分为安静对照组和运动后即刻组，运动后24 h组。安静对照组正常喂养，两个运动组进行一次性大负荷离心运动，分别取对照组腓肠肌和运动组运动后0和24 h的腓肠肌，使用冰冻切片原位杂交法检测αB-晶状体蛋白基因表达情况。
结果与结论：骨骼肌细胞αB-晶状体蛋白基因在对照组呈低水平表达，在运动后即刻和运动后24 h组均呈较高表达 (P < 0.05)，αB-晶状体蛋白基因表达杂交信号主要位于肌细胞膜下。结果证实，大强度离心运动可诱导骨骼肌细胞αB-晶状体蛋白基因表达增高。

关键词: 离心运动, αB-晶状体蛋白, 基因, 肌细胞, 组织工程

Abstract:

BACKGROUND: Skeletal muscle is called “molecular chaperones” the small heat shock protein αB-crystallin protein in the exercise training may have important physiological functions.
OBJECTIVE: To examine the mRNA expression of αB-crystallin in skeletal muscle after eccentric exercise.
METHODS: Adult male Wistar rats were randomly divided into control group, immediately after exercise group and 24 hours after exercise group. Control group received normal feeding. Exercise groups experienced once high-intensity eccentric exercise. After exercise, gastrocnemius muscle from excise groups at 0 and 24 hours after excise and those from control group were removed. The gastrocnemius muscles were processed for cryosection in situ hybridization to measure αB-crystallin mRNA expression.
RESULTS AND CONCLUSION: Compared with control group which had a weak level of labeling, the hybridization signal of αB-crystallin mRNA was obviously enhanced near muscle cell membrane in immediately after exercise group and 24 hours after exercise group (P < 0.05). Results suggest that αB-crystallin mRNA expression was up-regulated after high-intensity eccentric exercise.

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