中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (28): 5225-5228.doi: 10.3969/j.issn.1673-8225.2011.28.024

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

肝细胞生长因子对过氧化氢诱导肝细胞凋亡的影响

沈广海1，高  爽2，沈兆亮3，田丽敏1，唐  博4   

  1. 1锦州市传染病医院肝脏病科，辽宁省锦州市 121000
    2锦州市中心医院普外科，辽宁省锦州市 121000
    3辽宁医学院附属第一医院普外科，辽宁省锦州市  121001
    4大连医科大学附属第二医院普外三科，辽宁省大连市 116023
  • 收稿日期:2011-03-05 修回日期:2011-06-01 出版日期:2011-07-09 发布日期:2011-07-09
  • 通讯作者: 沈广海，男，1954年生，1980年哈尔滨医科大学毕业，辽宁省庄河市人，汉族，副教授，主要从事肝脏疾病的临床诊治及预后工作。 dytangbo@163. com
  • 作者简介:沈广海，男，1954年生，1980年哈尔滨医科大学毕业，辽宁省庄河市人，汉族，副教授，主要从事肝脏疾病的临床诊治及预后工作。 dytangbo@163. com

Effects of hepatocyte growth factors on H2O2-induced hepatocyte apoptosis

Shen Guang-Hai1, Gao Shuang2, Shen Zhao-liang2, Tian Li-min1, Tang Bo4   

  1. 1Department of Hepatology, Jinzhou Infection Hospital, Jinzhou  121000, Liaoning Province, China
    2Department of General Surgery, Jinzhou Central Hospital, Jinzhou   121000, Liaoning Province, China
    3Department of General Surgery, First Affiliated Hospital of Liaoning Medical University, Jinzhou  121001, Liaoning Province, China
    4Third Department of General Surgery, Second Affiliated Hospital of Dalian Medical University, Dalian  116023, Liaoning Province, China
  • Received:2011-03-05 Revised:2011-06-01 Online:2011-07-09 Published:2011-07-09
  • Contact: Shen Guang-hai, Associate professor, Department of Hepatology, Jinzhou Infection Hospital, Jinzhou 121000, Liaoning Province, China dytangbo@163.com
  • About author:Shen Guang-hai, Associate professor, Department of Hepatology, Jinzhou Infection Hospital, Jinzhou 121000, Liaoning Province, China dytangbo@163.com

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304

被引次数

3

摘要：

背景：肝细胞生长因子对多种细胞具有保护作用。
目的：观察肝细胞生长因子对过氧化氢诱导肝细胞凋亡的保护作用及其机制。
方法：采用人LO2肝细胞系，随机分成3组：正常对照组为正常培养的LO2细胞；模型组加入100 mmol/L过氧化氢作用LO2细胞4 h；肝细胞生长因子组加入50 mg/L 肝细胞生长因子预处理LO2细胞24 h，再加入100 mmol/L过氧化氢继续培养4 h后处理细胞。
结果与结论：体外培养的LO2细胞经100 mmol/L过氧化氢作用4 h后，LO2细胞可出现明显的凋亡现象，表现为细胞存活率降低(P < 0.01)，Caspase-3蛋白表达增加(P < 0.01)，Bcl-2蛋白表达降低(P < 0.01)。给予质量浓度50 mg/L 肝细胞生长因子预处理24 h后再加入100 mmol/L过氧化氢继续培养4 h，LO2细胞的凋亡被显著抑制(P < 0.01)，说明肝细胞生长因子可通过增加LO2细胞Bcl-2的表达来抑制过氧化氢诱导的LO2细胞凋亡。

关键词: 肝细胞生长因子, 细胞凋亡, Bcl-2, Caspase-3, 过氧化氢, 组织工程

Abstract:

BACKGROUND: Hepatocyte growth factors exhibit protective effects on many cells. 
OBJECTIVE: To investigate the protective effects of hepatocyte growth factors on H2O2-induced hepatocyte apoptosis of liver cells as well we the possible mechanism.
METHODS: LO2 liver cell lines were randomly divided into three groups: normal control group: LO2 cells were conventionally cultured; model group: LO2 cells were treated with 100 mmol/L H2O2 for 4 hours; hepatocyte growth factor group: LO2 cells were pretreated with 50 mg/L hepatocyte growth factors for 24 hours and then further cultured with 100 mmol/L H2O2 for 4 hours.
RESULTS AND CONCLUSION: After 4-hour treatment with 100 mmol/L H2O2, in vitro cultured LO2 cells showed obvious apoptosis, presenting with decreased cell survival rate (P < 0.01), increased caspase-3 protein expression (P < 0.01), and decreased Bcl-2 protein expression (P < 0.01). After 24-hour pretreatment with hepatocyte growth factors and subsequent 4-hour culture with 100 mmol/L H2O2, LO2 cell apoptosis was significantly inhibited (P < 0.01). These findings suggest that hepatocyte growth factors can inhibit H2O2-induced LO2 cell apoptosis by increasing Bcl-2 expression in LO2 cells.

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