中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (28): 5185-5188.doi: 10.3969/j.issn.1673-8225.2011.28.014

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

长期运动预适应模型大鼠心脏蛋白激酶C和热休克蛋白70的表达

孙晓娟，侯  娜   

  1. 咸阳师范学院体育系，陕西省咸阳市 712000
  • 收稿日期:2011-03-03 修回日期:2011-06-03 出版日期:2011-07-09 发布日期:2011-07-09
  • 作者简介:孙晓娟☆，1973年生，女，陕西省彬县人，讲师，2008年于上海体育学院毕业，博士主要从事运动与心血管形态和机能的研究工作。 sxj362@yahoo. com.cn
  • 基金资助:

    陕西省教育厅科学研究计划项目(09JK805)；2010年陕西省体育局体育科研课题(10050)；咸阳师范学院专项科研基金资助重点项目(09XSYK103)。

Expression of protein kinase C and heat shock protein 70 in the heart of rat models of long-term exercise preconditioning

Sun Xiao-juan, Hou Na   

  1. Department of Physical Education, Xianyang Normal University, Xianyang 712000, Shaanxi Province, China
  • Received:2011-03-03 Revised:2011-06-03 Online:2011-07-09 Published:2011-07-09
  • About author:Sun Xiao-jun☆, Doctor, Lecturer, Department of Physical Education, Xianyang Normal University, Xianyang 712000, Shaanxi Province, China sxj362@yahoo.com. cn
  • Supported by:

    Science Research Plan Program of Education Department of Shaanxi Province, No.09JK805*; Sport Science Research Program of Sport Bureau of Shaanxi Province in 2010, No. 10050*; Science Research Foundation for Xianyang Normal University, No. 09XSYK103*

PDF

278

被引次数

9

摘要：

背景：研究表明蛋白激酶C和热休克蛋白70可能参与运动预适应的心脏保护作用。
目的：观察长期运动预适应对大鼠心脏蛋白激酶C和热休克蛋白70的影响及其对心脏保护的作用机制。
方法：将SD大鼠随机分为对照组，力竭运动组和运动预适应组。对照组和力竭运动组大鼠常规饲养3周，运动预适应组大鼠进行3周的间歇性游泳运动建立长期运动预适应动物模型。3周后，力竭运动组和运动预适应组大鼠进行一次性力竭游泳运动。
结果与结论：大鼠力竭运动后，力竭运动组心脏热休克蛋白70表达高于对照组(P < 0.05)；先经3周运动预适应再进行力竭运动后，运动预适应组心脏蛋白激酶C和热休克蛋白70的表达高于力竭运动组(P < 0.05)。结果证实，长期运动预适应能激活心脏蛋白激酶C，诱导热休克蛋白70的合成增多，从而发挥其心脏保护作用。

关键词: 运动预适应, 蛋白激酶C, 热休克蛋白70, 心脏, 大鼠, 组织工程

Abstract:

BACKGROUND: Several studies have demonstrated that protein kinase C (PKC) and heat shock protein 70 (HSP70) take part in the cardioprotection of exercise preconditioning (EP), but the relationship and the underlying mechanism remain poorly understood.
OBJECTIVE: To investigate the effects of long-term EP on PKC and HSP70 expression in the rat heart and the possible mechanism of cardioprotection.
METHODS: Sprague-Dawley rats were randomly divided into the control, exhausted exercise and EP groups. Rats in the control and exhausted exercise groups were fed normally for 3 weeks. The EP group rats underwent 3 weeks of intermittent swimming exercise in order to establish long-term EP animal model. After 3 weeks, the exhausted exercise group and exercise preconditioning group underwent exhausted exercise. PKC and HSP70 expression in rat heart was determined by western blot analysis and immunohistochemistry.
RESULTS AND CONCLUSION: After exhausted exercise, cardiac HSP70 expression was greater in the exhausted exercise group than in the control group (P < 0.05). After 3 weeks of EP and subsequent exhausted exercise, cardiac PKC and HSP70 expression was greater in the EP group than in the exhausted exercise group (P < 0.05). The results demonstrated that long-term EP could activate cardiac PKC expression and induce HSP70 synthesis, which could play a big role in cardioprotection of long-term EP.

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