中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (27): 5096-5099.doi: 10.3969/j.issn.1673-8225.2011.27.035

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

MicroRNA在干细胞增殖与分化过程中的调控作用

杞少华1，武栋成1，李东升2   

  1. 1武汉大学基础医学院生物化学与分子生物学系，湖北省武汉市430071
    2湖北省胚胎干细胞重点实验室，湖北省十堰市442000
  • 收稿日期:2011-01-26 修回日期:2011-03-10 出版日期:2011-07-02 发布日期:2011-07-02
  • 通讯作者: 武栋成，教授，博士生导师，武汉大学基础医学院生物化学与分子生物学系，湖北省武汉市 430071 bcdcwu@hotmail.com 并列通讯作者：李东升，教授，湖北省胚胎干细胞重点实验室，湖北省十堰市442000 dsli@yymc.edu.cn
  • 作者简介:杞少华☆，女，湖北省十堰市人，汉族，武汉大学基础医学院生物化学与分子生物学系在读博士，主要从事干细胞移植方面研究。 qshhua@tom.com
  • 基金资助:

    课题受武汉大学科研基金(301275530, 301275685)的资助。

Roles of MicroRNA in regulating proliferation and differentiation of stem cells

Qi Shao-hua1, Wu Dong-cheng1, Li Dong-sheng2   

  1. 1Department of Biochemistry and Molecular Biology, Wuhan University Basic Medical College, Wuhan  430071, Hubei Province, China
    2Hubei Key Laboratory of Embryonic Stem Cells, Shiyan  442000, Hubei Province, China
  • Received:2011-01-26 Revised:2011-03-10 Online:2011-07-02 Published:2011-07-02
  • Contact: Wu Dong-cheng, Professor, Doctoral supervisor, Department of Biochemistry and Molecular Biology, Wuhan University Basic Medical College, Wuhan 430071, Hubei Province, China; bcdcwu@hotmail.com Correspondence to: Li Dong-sheng, Professor, Hubei Key Laboratory of Embryonic Stem Cells, Shiyan 442000, Hubei Province, China dsli@yymc.edu.cn
  • About author:Qi Shao-hua☆, Studying for doctoral degree, Department of Biochemistry and Molecular Biology, Wuhan University Basic Medical College, Wuhan 430071, Hubei Province, China qshhua@tom.com
  • Supported by:

    Science and Technology Fund by Wuhan University, No. 301275530*, 301275685*

PDF

472

被引次数

10

摘要：

背景：研究表明MicroRNA(miRNA)可通过抑制干细胞特定mRNA序列的翻译来调控干细胞的自我更新和分化。
目的：探讨miRNAs在干细胞增殖和分化过程中的作用。
方法：由第一作者检索2000/2010 PubMed数据库、Elsevier数据库及Nature数据库。英文检索词为“stem cell，embryonic stem cell(ESC)， induced pluripotent stem cells(iPS cell)， microRNA(miRNA)”。排除重复性研究。共保留其中的39篇进行归纳总结。
结果与结论：胚胎干细胞有特异性的miRNAs表达，miRNAs对胚胎干细胞增殖与分化起重要的调控作用；miRNAs对造血干细胞分化的多个阶段和方向有调控作用；miRNAs还参与了神经干细胞、间充质干细胞和皮肤干细胞等成体干细胞分化的调控。干细胞特异性的miRNAs可提高体细胞重编程的效率。

关键词: 干细胞, 增殖, 分化, 调控, microRNA(miRNA)

Abstract:

BACKGROUND: MicroRNAs (miRNAs) have an important role in regulating stem cell self-renewal and differentiation by repressing the translation of selected mRNAs in stem cells.
OBJECTIVE: To investigate the roles of miRNAs in regulating proliferation and differentiation of stem cells.
METHODS: The first author searched PubMed database, Elsevier database and Nature database from 2000 to 2010. Key words were “stem cell, embryonic stem cell (ESC), induced pluripotent stem cell (iPS cell), microRNA (miRNA)”. Duplicated articles were excluded. Finally, 39 articles were selected.
RESULTS AND CONCLUSION: MicroRNAs are specifically expressed in embryonic stem cells. MiRNAs play important roles in regulating stem cell’s proliferation and differentiation. MiRNAs modulate multiple stages and cell types of hemopoietic stem cell differentiation. MiRNAs also regulate the differentiation of neural stem cells, mesenchymal stem cells and skin stem cells. Embryonic stem cell-specific miRNAs promote the efficiency of somatic cell reprogramming.

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