中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (27): 5087-5090.doi: 10.3969/j.issn.1673-8225.2011.27.033

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

肿瘤干细胞的分离培养及生物学特性

王瑞海   

  1. 唐山市开平医院，河北省唐山市 063000
  • 收稿日期:2011-04-28 修回日期:2011-06-06 出版日期:2011-07-02 发布日期:2011-07-02
  • 作者简介:王瑞海，男，1974年生，河北省唐山市人，汉族，2010年河北医科大学毕业，主治医师，主要从事肿瘤的临床治疗工作。 wangruihai1974@163.com

Isolation, culture and biological characteristics of cancer stem cells

Wang Rui-hai   

  1. Kaiping Hospital of Tangshan City, Tangshan  063000, Hebei Province, China
  • Received:2011-04-28 Revised:2011-06-06 Online:2011-07-02 Published:2011-07-02
  • About author:Wang Rui-hai, Attending physician, Kaiping Hospital of Tangshan City, Tangshan 063000, Hebei Province, China wangruihai1974@163.com

PDF

901

被引次数

10

摘要：

背景：肿瘤干细胞是一类具有自我更新能力、不定分化潜能的种子细胞，可以形成不同分化程度的肿瘤细胞, 是恶性肿瘤不断生长、复发转移、无法被传统的放化疗方法彻底杀死的根源。
目的：总结肿瘤干细胞的分离培养及其生物学特性。
方法：由作者采用电子检索的方式，在万方数据库中检索以“肿瘤干细胞，分离培养，生物学特性” 为中文关键词，计算机检索1990-01/2010-12有关肿瘤干细胞的分离培养及生物学特性的文章，经检索共查到相关文献45篇。经阅读标题、摘要、全文后，排除内容重复、普通综述、Meta分析类文章后，筛选纳入22篇文献进行评价。
结果与结论：在肺癌、胰腺癌、结肠癌、肝细胞癌、神经系统肿瘤等多种脏器肿瘤中，CD133被作为鉴定肿瘤干细胞的特异性标记物之一。巢蛋白、波形蛋白和CD117在神经系统肿瘤干细胞中有表达。CD44及内皮素转化酶在肺癌肿瘤干细胞中的表达具有意义。CD44和CD24可作为分选人胰腺癌细胞株PANC-1中肿瘤干细胞的表面标志。而CD166在大肠癌肿瘤干细胞中高表达。

关键词: 肿瘤干细胞, 分离, 培养, 生物学特性, 干细胞

Abstract:

BACKGROUND: Cancer stem cells are a kind of seed cells with self-renewal capacity and uncertain differentiation potential, and can differentiate into tumor cells to different extents, which are the root cause of continued growth, metastasis and recurrence of malignant tumors which cannot be completely killed by traditional chemotherapy.
OBJECTIVE: To review the isolation, culture and biological characteristics of cancer stem cells.
METHODS: A computer retrieve of Wanfang database (1990-01/2010-12) was conducted for articles regarding the isolation, culture and biological characteristics of cancer stem cells using the keywords of “cancer stem cells, isolation and culture, biological characteristics” in Chinese. Repetitive articles, reviews and Meta analyses were excluded, and finally 22 of 45 articles were included in result analysis.
RESULTS AND CONCLUSION: CD133 is regarded as a specific marker of cancer stem cells in various organ tumors, including lung cancer, pancreatic cancer, colon cancer, hepatocellular carcinoma, nervous system tumors. Nestin, vimentin, and CD117 express in cancer stem cells of the nervous system. Expressions of CD44 and endothelin-converting enzyme in lung cancer stem cells are of significance. CD44 and CD24 can be used as cancer stem cell surface markers for sorting human pancreatic cancer cell line PANC-1. CD166 has a high expression in colorectal cancer stem cells.

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