中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (23): 4237-7241.doi: 10.3969/j.issn.1673-8225.2011.23.014

• 脂肪干细胞 adipose-derived stem cells • 上一篇    下一篇

体外诱导脂肪源性干细胞向类肝细胞的定向分化

刘  剑1，李  立2，冉江华2，张升宁2，邵剑春3   

  1. 1昆明医学院，云南省昆明市  650031
    2昆明医学院附属甘美医院肝胆胰外科，云南省昆明市  650011
    3昆明市甘美医院检验科，云南省昆明市650011
  • 收稿日期:2011-02-26 修回日期:2011-05-09 出版日期:2011-06-04 发布日期:2011-06-04
  • 通讯作者: 李立，教授，主任医师，昆明医学院附属甘美医院肝胆胰外科，云南省昆明市 650011 ynkmlili@yahoo.com
  • 作者简介:刘剑☆，男，1973年生，云南省昆明市人，汉族，昆明医学院在读博士，主治医师，主要从事普通外科肝胆专业研究。 liujianwxc1219@163.com
  • 基金资助:

    云南省科技厅科研基金资助项目(2007CA007)：“多脏器联合移植重大关键技术的研究与应用示范”。

Directional differentiation of adipose tissue-derived stem cells into hepatic-like cells in vitro by procedure-induction

Liu Jian1, Li Li2, Ran Jiang-hua2, Zhang Sheng-ning2, Shao Jian-chun3   

  1. 1Kunming Medical College, Kunming  650031, Yunnan Province, China
    2Department of Hepatopancreatobiliary Surgery, Ganmei Hospital Affiliated to Kunming Medical College, Kunming  650011, Yunnan Province, China
    3Department of Clinical Laboratory, Ganmei Hospital, Kunming  650011, Yunnan Province, China
  • Received:2011-02-26 Revised:2011-05-09 Online:2011-06-04 Published:2011-06-04
  • Contact: Li Li, Professor, Chief physician, Department of Hepatopancreatobiliary Surgery, Ganmei Hospital Affiliated to Kunming Medical College, Kunming 650011, Yunnan Province, China ynkmlili@yahoo.com
  • About author:Liu Jian☆, Studying for doctorate, Attending physician, Kunming Medical College, Kunming 650031, Yunnan Province, China liujianwxc1219@163.com
  • Supported by:

    the Scientific Research Foundation of Department of Science and Technology of Yunnan Province, No. 2007CA007*

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摘要：

背景：用脂肪源性干细胞治疗肝脏疾病之前，如何建立有效稳定的肝细胞分化诱导方案，纯化并快速扩增性能稳定的类肝细胞等问题亟待解决。
目的：建立大鼠脂肪源性干细胞转化为类肝细胞的程序化诱导体系。
方法：分离纯化Lewis大鼠脂肪源性干细胞，流式细胞仪鉴定其表面标志，分3个阶段加入含有肝细胞生长因子、成纤维细胞生长因子4、酸性成纤维细胞生长因子、制瘤素M细胞因子的诱导培养体系，使脂肪源性干细胞向肝细胞转化。
结果与结论：大鼠脂肪源性干细胞诱导7，14，21 d后，细胞阳性表达 ALB、AFP、CK18mRNA，表达量随诱导时间延长而增强，类肝细胞具有白蛋白合成功能。氨代谢和尿素的合成功能在9~12 d出现并持续存在。结果表明脂肪源性干细胞体外分段诱导可成功转化为类肝细胞。

关键词: 脂肪源性干细胞, 分化, 类肝细胞, 程序化诱导, 大鼠

Abstract:

BACKGROUND: How to establish effective stable hepatic cell differentiation schedule, to purify or rapidly amplify stable hepatic-like cells needs to be solved before adipose tissue-derived stem cells (ADSCs) were used to treat liver diseases.
OBJECTIVE: To establish sequencing induction system of ADSCs transforming into hepatic-like cells.
METHODS: After isolated and purified the Lewis rat ADSCs, the surface marks of ADSCs were identified by flow cytometry. The rat ADSCs were transformed into the hepatic-like cells in the procedure-culture system by tris-step including hepatocyte growth factor (FGF), fibroblast growth factor-4, acid fibroblast growth factor and oncostatin M cytokine.
RESULTS AND CONCLUSION: After rat ADSCs were induced at 7, 14 and 21 days, the expression of albumin (ALB), alpha fetoprotein (AFP) and CK18 mRNA was determined and fortified over time. Hepatic-like cells had ALB synthesis function. Metabolism of ammonia and urea synthesis occurred and lasted after 9-12 days. Results suggest ADSCs were successfully differentiated into hepatic-like cells in vitro after procedure-induction.

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