中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (18): 3351-3353.doi: 10.3969/j.issn.1673-8225.2011.18.029

• 器官移植动物模型 organ transplantation and animal model • 上一篇    下一篇

大鼠胆管缺血狭窄模型的构建

赵德芳1，张  彤1，赵利军2，陈大志3   

  1. 1内蒙古医学院附属医院肝胆外科，内蒙古自治区呼和浩特市 010059
    2华北煤炭医学院病理生理学教研室，河北省唐山市 063000
    3北京朝阳医院肝胆外科，北京市 100021
  • 收稿日期:2010-12-03 修回日期:2011-03-06 出版日期:2011-04-30 发布日期:2011-04-30
  • 通讯作者: 陈大志，博士，北京朝阳医院肝胆外科，北京市 100021 dazhichen@ medmail.com
  • 作者简介:赵德芳☆，男，1974年生，内蒙古自治区科左中旗人，蒙古族， 2008年首都医科大学毕业，博士，副主任医师，从事肝胆外科及肝移植研究。 hostmanabc@ hotmail.com

Establishment of a rat model of ischemic biliary strictures induced by clamping

Zhao De-fang1, Zhang Tong1, Zhao Li-jun2, Chen Da-zhi3   

  1. 1Department of Hepatobiliary Surgery, Affiliated Hospital of Inner Mongolia Medical College, Hohhot  010059, Inner Mongolia Autonomous Region, China
    2Department of Pathophysiology, Medical College, Hebei United University, Tangshan  063000, Hebei Province, China
    3Department of Hepatobiliary Surgery, Beijing Chaoyang Hospital, Beijing  100021, China
  • Received:2010-12-03 Revised:2011-03-06 Online:2011-04-30 Published:2011-04-30
  • Contact: Chen Da-zhi, Doctor, Department of Hepatobiliary Surgery, Beijing Chaoyang Hospital, Beijing 100021, China
  • About author:Zhao De-fang☆, Doctor, Associate chief physician, Department of Hepatobiliary Surgery, Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010059, Inner Mongolia Autonomous Region, China hostmanabc@hotmail.com

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269

被引次数

2

摘要：

背景：有研究表明可以用钳夹法制作心肌缺血再灌注损伤模型。
目的：采用钳夹法建立大鼠胆管缺血狭窄动物模型。
方法：将Wistar大鼠随机分为假手术组和模型组，模型组用2枚显微血管夹夹闭一段长约0.8 cm的胆总管90 min，假手术组仅暴露胆管未夹闭。术后21 d两组大鼠均经静脉胆道造影，下腔静脉取血，分离血清进行总胆红素及直接胆红素浓度测定。
结果与结论：模型组钳夹部位以上胆管明显扩张，静脉胆管造影示胆管扩张，壁光滑，狭窄部位以下胆管未显影。3只(16.7%)胆道未显影，但解剖动物时发现其肝脏肿大，微呈绿色，钳夹处胆总管闭锁，胆总管扩张。模型组血清总胆红素及直接胆红素高于假手术组(P < 0.05)。结果证实，实验采用血管夹钳闭缺血法成功构建了大鼠胆管缺血狭窄模型。

关键词: 静脉胆道造影, 再灌注损伤, 胆总管, 胆红素, 动物模型, 组织构建

Abstract:

BACKGROUND: Studies have showed that forceps operation can be used to prepare myocardial ischemia-reperfusion injury models.
OBJECTIVE: To prepare a rat biliary ischemic stenosis model using forceps operation.
METHODS: After the optimal time of biliary ischemia was determined, 40 Wistar rats were randomly divided into 2 groups. 0.8 cm common bile duct was clamped for 90 minutes with 2 micro-vessel clips in the experimental group. The common bile duct was not clamped in the sham operation group. Twenty-one days later, the intravenous cholangiography was conducted in all the rats. Blood samples were collected from the inferior vena cava to investigate the serum concentration of total bilerubin (TBIL) and direct bilerubin (DBIL).
RESULTS AND CONCLUSION: Twenty-one days later: 3 rats in experimental group could be evidenced to bile duct distension by intravenous cholangiography. But they all had serious dilatation, and their liver was bad when autopsy was done. Obvious dilatation of the common bile duct appeared over the clamped site. There was no dilatation of the common bile duct in the sham operation group. The serum concentration of TBIL and DBIL were significantly higher in the experimental group than in the sham operation group (P < 0.05). The result proved that The rat model of ischemic biliary strictures resulting from clamping has been successfully established.

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