中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (34): 6351-6354.doi: 10.3969/j.issn.1673-8225.2010.34.019

• 药物控释材料 drug delivery materials • 上一篇    下一篇

可吸收性多聚糖止血材料PerClot®对颅骨愈合的影响

牛  雯，刘  毅，张  博，罗  颖，刘曼玲，李志超   

  1. 解放军第四军医大学基础部病理与病理生理学教研室，陕西省西安市  710032
  • 出版日期:2010-08-20 发布日期:2010-08-20
  • 通讯作者: 李志超，教授，博士研究生导师，第四军医大学基础部病理与病理生理学教研室，陕西省西安市 710032
  • 作者简介:牛雯★，女，1982年生，陕西省蒲城县人，汉族， 2009年解放军第四军医大学毕业，硕士，助理实验师，主要从事骨组织工程学方面的研究。 pathophy@fmmu.edu.cn

Effects of an absorbable polysaccharide hemostat PerClot® on fracture healing of the cranial bone

Niu Wen, Liu Yi, Zhang Bo, Luo Ying, Liu Man-ling, Li Zhi-chao   

  1. Department of Pathology and Pathophysiology, Basic Section, Fourth Military Medical University of Chinese PLA, Xi’an  710032, Shannxi Province, China
  • Online:2010-08-20 Published:2010-08-20
  • Contact: Li Zhi-chao, Professor, Doctoral supervisor, Department of Pathology and Pathophysiology, Basic Section, Fourth Military Medical University of Chinese PLA, Xi’an 710032, Shannxi Province, China
  • About author:Niu Wen★, Master, Assistant experimentalist, Department of Pathology and Pathophysiology, Basic Section, Fourth Military Medical University of Chinese PLA, Xi’an 710032, Shannxi Province, China pathophy@fmmu.edu.cn

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摘要：

背景：PerClot®是一种来源于纯化的植物淀粉、经特殊工艺制成的可吸收性多聚糖止血颗粒，可使血液成分(纤维蛋白和血小板)聚集，有利于原始骨痂的形成。
目的：观察来源于变性淀粉的可吸收性多聚糖止血材料PerClot®对颅骨愈合的影响。
方法：12只健康成年新西兰兔，每只顶骨钻2个圆形缺损孔洞，共24个。按照填充材料的不同随机分别为对照组、Perclot®组和骨蜡组，8个/组。Perclot®组和骨蜡组缺损处覆盖淀粉型可吸收多聚糖止血粉和骨蜡，对照组不使用任何材料。术后6周处死动物，取原缺损边缘扩展至少1.5 cm范围的颅骨，大体观察骨质愈合情况进行愈合评分。应用图像分析软件测量骨小梁和骨缺如面积。
结果与结论：缺损处愈合评分，评分越小，愈合越好，结果显示Perclot®组明显低于对照组(P < 0.05)，骨蜡组与对照组比较无差异(P > 0.05)；与对照组比较，Perclot®组骨小梁面积明显增高(P < 0.05)，缺如面积明显降低(P < 0.05)。可见，Perclot®可吸收多聚糖止血粉有促兔颅骨愈合作用。

关键词: 可吸收多聚糖止血粉, 止血材料, 颅骨, 骨蜡, 愈合, 兔

Abstract:

BACKGROUND: PerClot® is an absorbable polysaccharide hemostat which is made from plant starch. It enables blood components such as fibrin and platelet to congregate, and then it benefits the formation of primary callus.
OBJECTIVE: To observe the effects of PerClot®, an absorbable polysaccharide hemostats, on fracture healing of the cranial bone.
METHODS: Twelve healthy adult New Zealand rabbits were used. Each of them was drilled into two circle cavities in its parietal bone, totaling 24. According to various stuff materials, twelve cavities were equally and randomly divided into three groups: control group, PerClot® group and bone wax group. The defect sites in the PerClot® group and bone wax group were covered with absorbable polysaccharide hemostats and bone wax, respectively. Control group was left intact. After six weeks of treatments, animals were killed. Tissue materials were drawn from the former defective spaces including at least 1.5 cm extention of the cranial bone. Bone healing was generally observed and graded. Bone trabecula and congenital absence areas were measured using the imaging analysis software.
RESULTS AND CONCLUSION: The lower healing score indicated the better healing. The score was significantly lower in the PerClot® group than in the control group (P < 0.05). No significant difference was detected between bone wax group and control group (P > 0.05). Compared with the control group, bone trabecula area was significantly increased (P < 0.05), congenital absence area was significantly reduced (P < 0.05) in the PerClot® group. In conclusion, PerClot® absorbable polysaccharide hemostats can enhance the fracture healing of the cranial bone in rabbits.

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