中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (33): 6095-6098.doi: 10.3969/j.issn.1673-8225.2010.33.004

• 骨组织构建 bone tissue construction • 上一篇    下一篇

胰岛素样生长因子1对小鼠成骨细胞增殖和碱性磷酸酶活性的影响

郭  玲1，王  敏2，郝  亮2   

  1. 1 泸州医学院附属口腔医院修复科，四川省泸州市 646000；2 四川大学华西口腔医学院修复科，四川省成都市  610041
  • 出版日期:2010-08-13 发布日期:2010-08-13
  • 通讯作者: 王敏，教授，博士生导师，四川大学华西口腔医院修复科，四川省成都市 610041 hxkqwangm@163.com
  • 作者简介:郭 玲★，女，1977年生，汉族，重庆人，2008年四川大学华西口腔医学院毕业，硕士，讲师，主要从事口腔修复方面的研究。
  • 基金资助:

    四川省卫生厅基金资助项目(编号：20090201)。课题名称：IGF-1与Wnt/β-catenin信号通路在糖尿病颌骨骨质疏松的作用研究。

Influence of insulin-like growth factor-1 on mouse osteoblast proliferation and alkaline phosphatase activity

Guo Ling1, Wang Min2, Hao Liang2   

  1. 1 Department of Prosthodontics, Hospital of Stomatology Affiliated to Luzhou Medical College, Luzhou   646000, Sichuan Province, China; 2 Department of Prosthodontics, West China School of Stomatology, Sichuan University, Chengdu   610041, Sichuan Province, China
  • Online:2010-08-13 Published:2010-08-13
  • Contact: Wang Min, Professor, Doctoral supervisor, Department of Prosthodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China hxkqwangm@163.com
  • About author:Guo Ling★, Master, Lecturer, Department of Prosthodontics, Hospital of Stomatology Affiliated to Luzhou Medical College, Luzhou 646000, Sichuan Province, China
  • Supported by:

    the Foundation of Health Department of Sichuan Province, No. 20090201* 

PDF

559

被引次数

53

摘要：

背景：胰岛素样生长因子1是骨形成的调节因子，具有促进有丝分裂、促进成骨等作用。由骨细胞所产生的胰岛素样生长因子1在调节成骨细胞和破骨细胞介导的骨骼重建中起着重要的作用。
目的：观察胰岛素样生长因子1作用后，小鼠成骨细胞的增殖和碱性磷酸酶的表达。
方法：在体外培养的小鼠成骨细胞中分别加入25，50，100及200 μg/L的胰岛素样生长因子1，于培养的第1，2，3，4，5天用Cell Counting Kit-8比色法检测细胞增殖率；用流式细胞仪检测细胞的增殖周期和凋亡率；于培养的第3，6，9天应用酶联免疫法检测细胞内碱性磷酸酶活性。
结果与结论：胰岛素样生长因子1质量浓度在25~200 μg/L时，对小鼠成骨细胞的增殖率有明显的促进作用(P < 0.05)，且呈明显的浓度依赖效应。当胰岛素样生长因子1质量浓度在200 μg/L时，能明显提高细胞S期所占的比例。说明胰岛素样生长因子1可加速细胞的增殖活动，以保证参与骨改建的成骨细胞数量而促进骨组织再生。同时，胰岛素样生长因子1在25~200 μg/L时，能显著提高细胞内碱性磷酸酶活性(P < 0.05)，促进成骨细胞分化。

关键词: 胰岛素样生长因子1, 成骨细胞, 增殖, 碱性磷酸酶, 糖尿病性骨病

Abstract:

BACKGROUND: Insulin-like growth factor-1 (IGF-1) is a growth factor to promote mitosis and regulate bone formation. It is of great significance on the dynamic process of osteoclast-driven bone resorption and osteoblast-mediated bone formation.
OBJECTIVE: To elucidate the effect of IGF-1 on proliferation of mouse osteoblasts cultured in vitro and alkaline phosphatase activities.
METHODS: Mouse osteoblasts cultured in vitro were added into medium containing various concentrations of IGF-1 (25, 50, 100, and 200 μg/L). Cell proliferation was determined using Cell Counting Kit-8 method; Cell cycle and apoptosis was assayed with flow cytometry. Alkaline phosphatase activity was examined with enzyme-linked immunosorbent assay at 3, 6 and 9 days following culture.
RESULTS AND CONCLUSION: All IGF-1 concentrations used (25-200 μg/L) were effective to promote the proliferation of mouse osteoblasts (P < 0.05), showing significant concentration-dependent effects. The maximum response was obtained with 200 μg/L, at which of IGF-1 could notably increase the percentage of cells at S phase, which indicated that IGF-1 can accelerate cellular proliferation to ensure the number of osteoblasts that participated bone rebuilding and to promote bone tissue regeneration. Simultaneously, IGF-1 at various concentrations (25-200 μg/L) could significantly enhance alkaline phosphatase activity (P < 0.05), and accelerate osteoblast differentiation.

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