中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (33): 6099-6102.doi: 10.3969/j.issn.1673-8225.2010.33.005

• 骨组织构建 bone tissue construction • 上一篇    下一篇

重组核因子κB活化因子受体蛋白对破骨细胞活性的抑制作用

张里程，张立海，黄  鹏，唐佩福   

  1. 解放军总医院骨科，北京市 100853
  • 出版日期:2010-08-13 发布日期:2010-08-13
  • 通讯作者: 唐佩福，博士，教授，博士研究生导师，解放军总医院骨科，北京市 100853 pftang301@126.com
  • 作者简介:张里程★，男，1982年生，河南省淮阳人，汉族，解放军军医进修学院在读硕士，医师，主要从事骨与软组织损伤的修复重建方面的研究。 zhanglcheng218@126.com
  • 基金资助:

    本研究是在国家自然科学基金项目(30672135)“p57 蛋白参与破骨细胞伪足小体形成的研究”及北京市自然科学基金(7072072)“RANK蛋白PLAD结构域对破骨细胞功能的影响” 共同资助下完成的。

Receptor activator of nuclear factor kappa B protein inhibits osteoclast activity 

Zhang Li-cheng, Zhang Li-hai, Huang Peng, Tang Pei-fu   

  1. Department of Orthopedics, General Hospital of Chinese PLA, Beijing  100853, China
  • Online:2010-08-13 Published:2010-08-13
  • Contact: Tang Pei-fu, Doctor, Professor, Doctoral supervisor, Department of Orthopedics, General Hospital of Chinese PLA, Beijing 100853, China pftang301@126.com
  • About author:Zhang Li-cheng★, Studying for master’s degree, Physician, Department of Orthopedics, General Hospital of Chinese PLA, Beijing 100853, China zhanglcheng218@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30672135*; the Natural Science Foundation of Beijing, No. 7072072*

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摘要：

背景：核因子κB活化因子受体直接参与破骨细胞的活性及功能调节，在骨吸收类疾病发病中具有重要意义。通过基因工程得到的可溶性核因子κB活化因子受体蛋白可能为防治骨质疏松等骨吸收类疾病提供了新的有效手段。
目的：观察重组重组核因子κB活化因子受体蛋白对体外培养破骨细胞活化、吸收活性的影响。
方法：取新生24 h内SD大鼠胎鼠的四肢长骨，机械分离获得破骨细胞，采用不同浓度重组重组核因子κB活化因子受体蛋白干预后，行抗酒石酸酸性磷酸酶染色及骨吸收陷窝甲苯胺蓝染色，观察其对破骨细胞的生长情况。
结果与结论：重组核因子κB活化因子受体蛋白对破骨细胞作用3 d后，破骨细胞数量明显减少，以10-4 mol/L重组核因子κB活化因子受体蛋白最为显著。核因子κB活化因子受体蛋白作用9 d时，骨片吸收陷窝数明显减少。由此认为，在体外重组核因子κB活化因子受体蛋白可以有效抑制破骨细胞活化及骨吸收活性。

关键词: 破骨细胞, 重组核因子&kappa, B, 活化因子受体蛋白, 骨质疏松, 骨吸收陷窝, 组织构建, 骨组织工程

Abstract:

BACKGROUND: Receptor activator of nuclear factor κB (RANK) plays an important role in the signaling pathways of osteoclasts differentiation and functional regulation. Therefore, it provides a new and effective method for bone resorption diseases.
OBJECTIVE: To investigate the effects of recombinant RANK protein on the activation and resorption activity of osteoclasts.
METHODS: Osteoclasts from SD rats were harvested and cultured, recombinant RANK protein with different concentrations was added into these culture systems. The growth of osteoclasts was observed by tartrate-resistant acid phosphatase and toluidine blue staining. 
RESULTS AND CONCLUSION: The number of osteoclasts was obvious decreased at 3 days after adding RANK protein, especially in the 10-4 mol/L group. The bone resorption pits were dramatically reduced at 9 days after adding RANK protein. Therefore, it suggested that recombinant RANK protein can effectively inhibit osteoclasts differentiation and resorption activity.

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