中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (28): 5141-5145.doi: 10.3969/j.issn.1673-8225.2010.28.003

• 骨组织构建 bone tissue construction • 上一篇    下一篇

复方司坦唑醇对D-半乳糖大鼠不同部位骨骼的影响

张新乐，吴  铁，崔  燎，何德谋，李  彬，张益嘉，吴光墚，戴娟秀   

  1. 广东医学院药理教研室，广东省湛江市  524023
  • 出版日期:2010-07-09 发布日期:2010-07-09
  • 通讯作者: 吴铁，教授，广东医学院药理教研室，广东省湛江市 524023
  • 作者简介:张新乐★，女，1979年生，广东省湛江市人，汉族， 2009年广东医学院毕业，硕士，实验师，主要从事骨药理学研究。 xinlezhang@tom.com
  • 基金资助:

    湛江市科技计划项目(2008C04016)。

Effect of compound stanozolol on different skeletal sites of rats induced by D-galactose

Zhang Xin-le, Wu Tie, Cui Liao, He De-mou, Li Bin, Zhang Yi-jia, Wu Guang-liang, Dai Juan-xiu   

  1. Department of Pharmacology, Guangdong Medical College, Zhanjiang  524023, Guangdong Province, China
  • Online:2010-07-09 Published:2010-07-09
  • Contact: Wu Tie, Professor, Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China
  • About author: Zhang Xin-le★, Master, Laboratory technician, Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China xinlezhang@tom.com
  • Supported by:

     Science and Technology Planning Project of Zhanjiang City, No. 2008C04016*

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428

被引次数

16

摘要：

背景：司坦唑醇能提高骨质疏松患者骨密度，具有促进骨形成和抑制骨吸收作用，但目前还没有报道显示其对老年性骨质疏松的效果如何，对不同部位骨骼的影响也未见报道。
目的：通过骨形态计量学观察复方司坦唑醇对D-半乳糖大鼠骨质疏松模型不同部位骨骼的影响。
方法：将SD大鼠以数字表法随机分为正常对照组、D-半乳糖模型组、复方司坦唑醇组。除正常对照组颈背部皮下注射生理盐水外，其余两组颈背部皮下注射D-半乳糖制备骨质疏松模型。正常对照组和D-半乳糖模型组灌胃给予溶剂对照，复方司坦唑醇组灌胃给予司坦唑醇0.54 mg/(kg•d)+吡拉西坦432 mg/(kg•d)，连续14周。测量胫骨上段松质骨和胫骨中段皮质骨骨组织形态计量学参数。
结果与结论：骨组织形态计量学参数显示，复方司坦唑醇可有效预防D-半乳糖对大鼠胫骨上段松质骨显微结构的破坏，抑制骨吸收，促进骨形成。复方司坦唑醇对D-半乳糖大鼠胫骨中段的皮质骨骨量丢失的作用不大，可抑制D-半乳糖大鼠皮质骨骨外膜的骨形成。

关键词: 复方司坦唑醇, D-半乳糖, 骨质疏松, 骨组织形态计量学, 骨组织工程

Abstract:

BACKGROUND: Stanozolol can increase bone mineral density in osteoporosis patients, which promotes bone formation and inhibiting bone resorption, however, its effectiveness in senile osteoporosis, as well as its effects on the different parts of the skeleton, remains unclearly.
OBJECTIVE: To study the effect of compound stanozolol on different skeletal sites of rat D-galactose-induced osteoporosis model through bone histomorphometry observation.
METHODS: Sprague Dawley rats were divided into the normal control, D-galactose and compound stanozolol groups according to random number table. All rats were prepared osteoporosis models by subcutaneous injecting D-galactose exception of the normal control group. In the compound stanozolol group, rats were gastric irrigated with stanozolol 0.54 mg/(kg•d)+Piracetam
432 mg/(kg•d) for 14 successive weeks. Solvent control was performed between normal control and D-galactose group. Bone histomorphometric parameter of the proximal tibial metaphysis (PTM), and tibial shaft was calculated. The biomechanic properties of right femur were analyzed by three-point bending test.
RESULTS AND CONCLUSION: Bone histomorphometric analysis showed that compound stanozolol could prevent micro-structural damage of PTM caused by D-galactose, inhibit bone resorption and facilitate proximal tibial bone formation. However, compound stanozolol had no obviously effect on cortical bone mass loss of tibial shaft by D-galactose.

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