中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (27): 5054-5057.doi: 10.3969/j.issn.1673-8225.2010.27.026

• 干细胞与中医药 stem cells and traditional Chinese medicine • 上一篇    下一篇

黄芩甙体外诱导大鼠骨髓基质细胞向神经干细胞的分化

张  雷1，张  媛1，倪红霞1，陈立梅2   

  1. 北华大学基础医学院，1形态实验室，2细胞生物教研室，吉林省吉林市  132013
  • 出版日期:2010-07-02 发布日期:2010-07-02
  • 通讯作者: 陈立梅，博士，副教授，北华大学基础医学院细胞生物教研室，吉林省吉林市 132013 limei.chen@ yahoo.com.cn
  • 作者简介:张 雷★，女，1971年生，吉林省吉林市人，汉族，1994年吉林师范大学毕业，硕士，实验师 主要从事细胞生物学与医学遗传学研究。 jlsunbin@126. com

Differentiation of rat bone marrow stromal cells into neural stem cells induced by baicalin in vitro

Zhang Lei1, Zhang Yuan1, Ni Hong-xia1, Chen Li-mei2   

  1. 1 Morphology Laboratory, 2 Department of Cytobiology, Medical College of Beihua University, Jilin  132013, Jilin Province, China
  • Online:2010-07-02 Published:2010-07-02
  • Contact: Chen Li-mei, Doctor, Associate professor, Department of Cytobiology, Medical College of Beihua University, Jilin 132013, Jilin Province, China limei.chen@yahoo. com.cn
  • About author:Zhang Lei★, Master, Experimentalist, Morphology Laboratory, Medical College of Beihua University, Jilin 132013, Jilin Province, China jlsunbin@126.com

PDF

337

被引次数

7

摘要：

背景：已有实验表明，骨髓基质细胞具有分化为骨骼、神经干细胞及造血干细胞的巨大潜能，而黄芩甙具有诱导细胞分化的作用。
目的：探讨黄芩甙体外诱导骨髓基质细胞分化为神经干细胞的可能性。
方法：分离培养Wistar纯系大鼠骨髓基质细胞，并将分离的骨髓基质细胞分为实验组和对照组，对照组不干预，实验组用黄芩甙(350~400 μmol/L)诱导，2组的培养环境相同。持续诱导6 d后选取生长良好的细胞进行蛋白质印迹和反转录PCR(RT-PCR)法检测。
结果与结论：黄芩甙诱导 7 d后，骨髓基质细胞形成较典型的神经细胞形态。诱导前骨髓基质细胞不表达神经细胞标记蛋白mRNA和蛋白；诱导 6 d表达神经细胞标记蛋白和mRNA；对照组不表达神经细胞标记蛋白和mRNA。证实黄芩甙在体外可诱导成年大鼠骨髓基质细胞分化为神经样细胞。

关键词: 黄芩甙, 骨髓基质细胞, 神经干细胞, 巢蛋白, 诱导

Abstract:

BACKGROUND: The existing experimental results show that bone marrow stromal cells have enormous potential of differentiating into bone, neural stem cells and hematopoietic stem cells, while baicalin can induce cell differentiation.
OBJECTIVE: To study the possibility that induced the differentiation of bone marrow stromal cells into neural stem cells in vitro by baicalin.
METHODS: Bone marrow stromal cells were harvested from Wistar rats, cultured and assigned to experimental and control groups. That in the control group was left intact. BMSCs in the experimental group were induced by baicalin (350-400 μmol/L). Both groups had the same training environment. Well grown cells following 6 consecutive days were detected using Western blot assay and reverse transcriptase-polymerase chain reaction (RT-PCR).
RESULTS AND CONCLUSION: After induced by baicalin for 7 days, bone marrow stromal cells formed the more typical form of neural cells. Before induction, bone marrow stromal cells did not express neural cell marker protein mRNA and protein. After induced for 6 days, neural cells expressed marker protein and mRNA. Control group was negative for marker protein and mRNA. These indicated that baicalin may induce the differentiation of adult rat bone marrow stromal cells into neuron-like cell in vitro.

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