中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (25): 4589-4593.doi: 10.3969/j.issn.1673-8225.2010.25.008

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

脱钙骨基质与生物蛋白胶复合载体修复兔关节软骨缺损

綦  惠，孙  磊，陈  磊，陶剑锋，江  健   

  1. 北京积水潭医院，北京市创伤骨科研究所，北京市  100035
  • 出版日期:2010-06-18 发布日期:2010-06-18
  • 通讯作者: 孙 磊，博士，副研究员，北京积水潭医院，北京市创伤骨科研究所，北京市 100035 dr.sunlei2009@gmail.com
  • 作者简介:綦 惠☆，女，1979年生，山东省泰安市人，汉族，2008年北京大学医学部毕业，博士，研究实习员，主要从事软骨组织工程与软骨细胞移植的研究。 kellyqhqh2002@163.com

Demineralized bone matrix compounded with biological fibrin glue for repairing rabbit cartilage defects

Qi Hui, Sun Lei, Chen Lei, Tao Jian-feng, Jiang Jian   

  1. Beijing Institute of Traumatology and Orthopedics, Beijing Jishuitan Hospital, Beijing   100035, China
  • Online:2010-06-18 Published:2010-06-18
  • Contact: Sun Lei, Doctor, Associate investigator, Beijing Institute of Traumatology and Orthopedics, Beijing Jishuitan Hospital, Beijing 100035, China dr.sunlei2009@gmail.com
  • About author:Qi Hui☆, Doctor, Beijing Institute of Traumatology and Orthopedics, Beijing Jishuitan Hospital, Beijing 100035, China kellyqhqh2002@163.com

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406

被引次数

9

摘要：

背景：应用组织工程学方法修复软骨组织缺损，需要有合适的载体，在发挥承载作用的同时对软骨细胞起到良好的固定作用。
目的：观察利用脱钙骨基质与生物蛋白胶的复合载体修复实验性兔关节软骨缺损的可行性及有效性。
方法：制备脱钙骨基质与生物蛋白胶的复合载体。建立兔关节软骨缺损模型，编号后，将42只新西兰大白兔随机分为3组：载体复合细胞组(n=15)：兔膝关节软骨缺损区植入含有软骨细胞的脱钙骨基质与生物蛋白胶的复合载体；单纯载体组(n=15)：植入不含细胞的复合载体；空白对照组(n=12)：不进行任何植入处理。依照分组，分别于术后4，8，12周取材，进行大体、组织学、甲苯胺蓝染色观察，并做Wakitani评分，观察各组动物关节缺损修复效果。
结果与结论：载体复合细胞组12周时可以修复膝关节软骨的缺损，并且以透明软骨组织为主，修复效果明显强于单纯载体组与空白对照组；Wakitani评分在各个时间均优于其他两组(P < 0.05)。提示脱钙骨基质与生物蛋白胶的复合载体负载软骨细胞可以作为软骨组织工程支架材料，能够用于再生修复软骨的缺损。

关键词: 脱钙骨基质, 生物蛋白胶, 组织工程, 软骨细胞, Wakitani评分

Abstract:

BACKGROUND: Cartilage defects can be treated with the method of tissue engineering, and the scaffold is of great importance in carrying vector and well fixation of chondrocytes.
OBJECTIVE: To investigate the feasibility and validity of using complex material of biological fibrin glue and demineralized bone matrix as a scaffold for cartilage defects repair in experimental rabbits.
METHODS: Scaffold was prepared with complex material of biological fibrin glue and demineralized bone matrix. Rabbit articular cartilage defect model was prepared and numbered, 42 New Zealand white rabbits were randomly divided into three groups: scaffold complex cell group (n=15): Rabbit articular cartilage defects were repaired with composite scaffold of biological fibrin glue and demineralized bone matrix seeded on the chondrocytes; single scaffold group (n=15): the defects were filled with complex scaffold without cells; blank control group (n=12): no any implant treatment was given. According to the grouping, the specimens were drawn at 4, 8, 12 weeks after surgery, and underwent gross, histological, toluidine blue staining, as well as Wakitani scores. The repair of articular defects was observed in each group.
RESULTS AND CONCLUSION: Implantation of scaffold with chondrocytes had the significant effect on the cartilage defects after 12 weeks, and hyaline cartilage was the predominant, the repair effect was superior to single scaffold and blank control groups. Wakitani score showed the best repairing results in the scaffold complex cell group (P < 0.05). Complex material of biological fibrin glue and demineralized bone matrix can be used as one of the optimal scaffolds for cartilage tissue engineering and it contributes to regeneration and repair of the cartilage defects.

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