中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (25): 4585-4588.doi: 10.3969/j.issn.1673-8225.2010.25.007

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

羟基丁酸-羟基辛酸共聚体/脱细胞软骨基质支架的细胞黏附性

张永红1，许  钰1，林  汲2，赵良启2   

  1. 1山西医科大学第二医院骨科，山西省太原市  030001；2山西大学生物技术研究所化学生物学与分子工程教育部重点实验室，山西省太原市  030006
  • 出版日期:2010-06-18 发布日期:2010-06-18
  • 作者简介:张永红☆，男，山西省忻州市人，汉族，1989年山西医科大学毕业，博士，硕士生导师，副主任医师，主要从事骨与软骨组织工程的研究。 yhzhy79106@163.com

Cell adhesion capability of poly(hydroxybutyrate-cohydroxyoctanoate)/acellular cartilage matrix scaffolds

Zhang Yong-hong1, Xu Yu1, Lin Ji2, Zhao Liang-qi2   

  1. 1 Department of Orthopedics, Second Hospital of Shanxi Medical University, Taiyuan   030001, Shanxi Province, China; 2 Key Laboratory of Chemical Biology and Molecular Engineering, Ministry of Education, Shanxi University Biotechnology Institute, Taiyuan   030006, Shanxi Province, China
  • Online:2010-06-18 Published:2010-06-18
  • About author:Zhang Yong-hong☆, Doctor, Master’s supervisor, Associate chief physician, Department of Orthopedics, Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China yhzhy79106@163.com

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526

被引次数

22

摘要：

背景：羟基丁酸-羟基辛酸共聚体[poly(hydroxybutyrate-co-hydroxyoctanoate)，PHBHOx]是一种新型的多聚羟基烷酸类材料，具有优良的可塑性、生物相容性、生物可降解性等性能，但同其他酯类材料相似，PHBHOx亲水性能较差，单纯PHBHOx材料支架细胞黏附性能明显不足。
目的：观察羟基丁酸-羟基辛酸共聚体/脱细胞软骨基质支架的细胞黏附性。
方法：取新鲜猪膝关节表面透明软骨，采用非离子型去污剂Triton X-100、低渗Tris-HCl以及Dna酶和Rna酶等进行脱细胞处理。低温粉碎后与PHBHOx以不同比例复合，采用溶剂浇铸-颗粒沥滤技术制备支架。分离培养猪关节软骨细胞，进行细胞-支架黏附实验并通过MTT比色法和扫描电镜观察软骨细胞在支架上的黏附存活情况。
结果与结论：PHBHOx/脱细胞软骨基质支架的细胞黏附率较对照组显著提高，软骨细胞在支架上黏附紧密，生长良好。提示复合脱细胞软骨基质可以明显提高单纯PHBHOx支架的细胞黏附性能。

关键词: 羟基丁酸-羟基辛酸共聚体, 脱细胞软骨基质, 支架材料, 细胞黏附性

Abstract:

BACKGROUND: The poly(hydroxybutyrate-co-hydroxyoctanoate) (PHBHOx) is a new type of polyhydroxylalkanoic acids materials, and has excellent plasticity, biocompatibility and biodegradability. However, as for other ester materials, the hydrophilic properties of PHBHOx is poor, the cell adhesion capability of PHBHOx scaffold is low.
OBJECTIVE: To investigate its cell adhesion capability of PHBHOx/acellular cartilage matrix (ACM) scaffolds.
METHODS: Fresh porcine articular cartilage from knee joints was sliced and then treated sequentially with Triton X-100, hypotonic solution Tris-HCl, DNA enzyme, RNA enzyme for decellularization. The ACM was shattered into powder at low temperature and composited with PHBHOx in different ratios to fabricate scaffold using solvent-casting, particulate-leaching method. Chondrocytes isolated from porcine articular cartilage were cultured and used in cell adhesion experiment. The adhesion and survival of chondrocytes on scaffolds were analyzed using MTT test and scanning electron microscopy.
RESULTS AND CONCLUSION: The cell adhesion rate of PHBHOx/ACM scaffold was significantly higher than the control group, chondrocytes adhered tightly and grew well on composite scaffold. All the results showed that ACM could significantly improve the cell adhesion capability of PHBHOx scaffold.

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