中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (21): 3873-3877.doi: 10.3969/j.issn.1673-8225.2010.21.017

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

聚碳酸亚丙酯/壳聚糖纳米纤维复合三维多孔支架的构建与性能

陈昊东1，赵剑豪1,2，曾  戎1,2，屠  美1,2，查振刚2,3   

  1. 1暨南大学理工学院材料系，广东省广州市 510632；2人工器官及材料教育部工程研究中心，广东省广州市  510632；3暨南大学第一附属医院骨科，广东省广州市 510630
  • 出版日期:2010-05-21 发布日期:2010-05-21
  • 通讯作者: 屠 美，教授，博士生导师，暨南大学材料科学与工程系，人工器官及材料教育部工程中心，广东省广州市 510632 tumei@jnu.edu.cn
  • 作者简介:陈昊东★，男，1982年生，安徽省庐江县人，暨南大学在读硕士，主要从事生物材料方面的研究。 chdwjs@foxmail.com
  • 基金资助:

    国家高薪技术发展计划(863计划)(2007AA09Z440)。课题名称：壳聚糖在人工关节大块骨缺损翻修中的应用研究。

Construction and property of three-dimensional poly(propylene carbonate)/chitosan nanofibers composite porous scaffolds

Chen Hao-dong1, Zhao Jian-hao 1,2, Zeng Rong 1,2, Tu Mei 1,2, Zha Zhen-gang 2,3   

  1. 1 Department of Materials Science and Engineering, Collage of Sciences and Engineering, Jinan University, Guangzhou  510632, Guangdong Province, China; 2 Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Guangzhou  510632, Guangdong Province, China;  3Department of Orthopaedics, First Affiliated Hospital of Jinan University, Guangzhou  510630, Guangdong Province, China
  • Online:2010-05-21 Published:2010-05-21
  • Contact: Tu Mei, Professor, Doctoral supervisor, Department of Materials Science and Engineering, Collage of Sciences and Engineering, Jinan University, Guangzhou 510632, Guangdong Province, China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Guangzhou 510632, Guangdong Province, China tumei@jnu.edu.cn
  • About author:Chen Hao-dong★, Studying for master’s degree, Department of Materials Science and Engineering, Collage of Sciences and Engineering, Jinan University, Guangzhou 510632, Guangdong Province, China chdwjs@foxmail.com
  • Supported by:

    the National “863” Program, No. 2007AA09Z440*

PDF

623

被引次数

37

摘要：

背景：聚碳酸亚丙酯(PPC)具有良好的力学性能和生物相容性，但是也存在合成高分子的共性不足即缺乏生物活性。壳聚糖纳米纤维具有优异的生物活性，但力学性能较差，很难保持稳定的高强度三维结构。
目的：将壳聚糖纳米纤维与聚碳酸亚丙酯复合，制备具有优异生物活性和良好力学性能的三维多孔组织工程支架。
方法：用溶液浇铸/粒子沥滤法制备PPC多孔支架，再用相分离法原位复合三维壳聚糖纳米纤维制备聚碳酸亚丙酯/壳聚糖纳米纤维复合三维多孔支架(PPC/CSNF)。用扫描电子显微镜观察PPC及PPC/CSNF多孔支架微观形态，并测定其压缩模量、孔隙率。用扫描电子显微镜观察PPC/CSNF多孔支架在新西兰大白兔大腿皮下埋植1，2个月后的细胞生长情况。
结果：PPC多孔支架孔径分布为200~500 μm且孔连通性好，PPC/CSNF多孔支架中的壳聚糖纳米纤维分布均匀其直径在50~500 nm之间；各种质量浓度的支架孔隙率均为90%以上；各种支架的压缩模量随着PPC浓度的增加而增加，最高可达约15 MPa；体内埋植的实验结果表明PPC/CSNF多孔支架具有良好的生物活性，可促进骨髓基质干细胞向软骨细胞分化。结果提示溶液浇铸/粒子沥滤法与低温相分离法相结合成功制备了力学性能与生物活性优异的PPC/CSNF多孔支架。该支架可促进新西兰大白兔的骨髓基质干细胞向软骨细胞分化。

关键词: 壳聚糖, 纳米纤维, 聚碳酸亚丙酯, 多孔支架, 生物材料

Abstract:

BACKGROUND: Poly(propylene carbonate) (PPC) is a new synthetic polyester with good mechanical properties and biocompatibility, but it also lacks of bioactivity as other synthetic polymers. Nanofibrous chitosan shows an excellent bioactivity. However, nanofibrous chitosan is hard to fabricate a three dimensional network with a high mechanical property.
OBJECTIVE: To fabricate three dimensional poly(propylene carbonate)/chitosan nanofibers (PPC/CSNF) composite porous scaffolds with good mechanical properties and bioactivity by combining PPC and CSNF.
METHODS: PPC porous scaffold was firstly fabricated by combining a solution-casting and a porogen-leaching technique, followed by in situ phase separation of chitosan solution within the PPC scaffold to form PPC/CSNF composite porous scaffold. The morphology of PPC and PPC/CSNF scaffolds were observed by a scanning electron microscope (SEM). The compressive modulus and porosity of the scaffolds were characterized. The cell growth after PPC/CSNF scaffold being subepidermally implanted in the leg of New Zealand rabbit for one and two months was also evaluated by SEM observation.
RESULTS AND CONCLUSION: A PPC porous scaffold with interconnected pores of 200-500 μm was fabricated. The diameter of chitosan nanofibers in the PPC/CSNF scaffold varied from 50 nm to 500 nm. All the scaffolds showed a high porosity of over 90%. The compressive modulus of various scaffolds increased with the concentration of PPC and reached a maximum of about 15 MPa, which met the mechanical requirement of cartilage tissue engineering scaffolds. The implant results showed that PPC/CSNF porous scaffold had good bioactivity and was able to induce the differentiation of bone marrow stromal cells of New Zealand rabbit into chondrocytes. A three dimensional PPC/CSNF composite porous scaffold with good mechanical properties and bioactivity was successfully fabricated by combing a phase separation and a solution-casting/porogen-leaching technique. The PPC/CSNF composite scaffold could promote the differentiation of bone marrow stromal cells of New Zealand rabbit into chondrocytes.

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