中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (15): 2727-2730.doi: 10.3969/j.issn.1673-8225.2010.15.016

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

槲皮素对大鼠卵巢发育和卵巢寿命的影响

庄小兰1，傅玉才2，许锦阶2，孔晓霞1，陈振国2，罗丽莉1   

  1. 1 汕头大学医学院第一附属医院妇科，广东省汕头市  515041；2汕头大学医学院细胞衰老实验室， 广东省汕头市 515041
  • 出版日期:2010-04-09 发布日期:2010-04-09
  • 通讯作者: 罗丽莉，教授，主任医师，汕头大学医学院第一附属医院妇科， 广东省汕头市 515041 jjxu@stu.edu.cn
  • 作者简介:庄小兰★，女， 1980年生， 江西省吉水县人，汉族，汕头大学医学院在读硕士，主要从事卵巢衰老调控研究。 zxl3511161@163.com
  • 基金资助:

    国家自然科学基金(30772318)。

Effects of quercetin on ovarian follicular development and ovarian life span in rats 

Zhuang Xiao-lan1, Fu Yu-cai2, Xu Jin-jie2, Kong Xiao-xia1, Chen Zhen-guo2, Luo Li-li1   

  1. 1 Department of Gynecology, First Affiliated Hospital of Medical College of Shantou University, Shantou  515041, Guangdong Province, China; 2 Laboratory of Cell Senescence, Medical College of Shantou University, Shantou  515041, Guangdong Province, China
  • Online:2010-04-09 Published:2010-04-09
  • Contact: Luo Li-li, Professor, Chief physician, Department of Gynecology, First Affiliated Hospital of Medical College of Shantou University, Shantou 515041, Guangdong Province, China jjxu@stu.edu.cn
  • About author:Zhuang Xiao-lan★, Studying for master’s degree, Department of Gynecology, First Affiliated Hospital of Medical College of Shantou University, Shantou 515041, Guangdong Province, China zxl3511161@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30772318*

PDF

453

被引次数

16

摘要：

背景：研究发现一些植物雌激素可改变生殖系统发育，并可能最终影响生殖衰老的年龄。槲皮素是一种黄酮类物质，具有微弱的雌激素效应，但对卵巢卵泡发育和卵巢衰老进程究竟有无影响还不清楚。
目的：观察槲皮素对大鼠卵巢卵泡发育和卵巢寿命的影响。
方法：健康成年SD 大鼠30只雌雄按2∶1合笼所生的新生雌鼠，分为3组：母鼠灌胃组，对孕10.5 d母鼠槲皮素灌胃，1次/d，直至分娩，取1，2，4 d新生雌鼠；腹腔注射组，对正常出生后12 h内新生雌鼠槲皮素腹腔注射，1次/d，取2，4 d新生雌鼠；空白对照组：不进行任何干预的1，2，4 d新生雌鼠；观察新生鼠卵巢发育情况。另取12月龄雌性大鼠22只，以数字表法随机分为实验组和对照组：实验组用槲皮素灌胃4个月，1次/d；对照组灌胃给予生理盐水。对灌胃前和灌胃期间12月龄雌性大鼠行阴道涂片，观察大鼠动情周期模式；灌胃结束后，计算卵巢和子宫系数，苏木精-伊红染色观察各期卵泡发育情况和卵泡总数变化。
结果与结论：用槲皮素干预1，2 d龄新生大鼠未装配的卵泡均减少，而发育更晚期阶段的卵泡即原始卵泡或发育卵泡有所增加。灌胃前大部分大鼠动情周期已经不规则，用药后，随着大鼠年龄的增长，实验组与对照组显示了相似的动情周期变化：规则的动情逐渐消失，周期变得延长或不规则，灌胃结束时均以不规则动情为主。灌胃4个月后，实验组大鼠卵巢大部分由闭锁卵泡组成，另外可见少许原始卵泡、发育卵泡以及窦状卵泡和黄体，实验组窦状卵泡较对照组增加(P < 0.05)，两组卵巢和子宫系数及体质量增加无差异。 提示槲皮素可能具有一定促进卵巢卵泡发育和成熟的作用，但不影响卵巢寿命。

关键词: 槲皮素, 卵泡发育, 卵巢寿命, 大鼠, 组织工程

Abstract:

BACKGROUND: Research demonstrated that some phytoestrogens can change the reproductive system development and may affect the age of reproductive senescence ultimately. Quercetin is a plant-derived flavonoid, which possess weak estrogen effect, however, there is limited evidence that quercetin may has effect on ovarian follicular development or ovarian consenescence.
OBJECTIVE: To explore the effects of quercetin on ovarian follicular development and ovarian life span in rats. 
METHOD: A total of 30 SD rats were caged together with male and female ratio of 2:1, and the neonatal female rates were divided into three groups. ①Intragastric perfusion group, pregnant rats were intragastric administrated quercetin (40 mg/kg.d) on day 10.5 after pregnancy till delivery, and the female rats with 1 d, 2 d and 4 d were selected; ②Intraperitoneal injection group, female neonatal rats were intraperitoneally injected with quercetin within 12 hours after birth, once per day, and 2 d and 4 d female rats were selected; ③Blank control group, 2 d and 4 d female rats were selected without intervention. The ovarian development of rats was observed. Additional 22 female rats were divided into the experimental and control groups according to the number table method. Rats in the experimental group were gastrogavage administered 40 mg/kg quercetin once per day for 4 consecutive months, and rats in the control group were fed with normal saline of same volume instead. Early aged rats were killed after completing administration, uerus and ovaries were weighed to calculate their coefficients and ovarian morphology were observed; In addition, vaginal smears were used to observe estrous cycles patterns.  
RESULTS AND CONCLUSION: The percentage of unassembled follicles was decreased and primordial follicles increased in the 1/2-day-old rat’s ovaries of intragastric administration group. The rats oestrus cycles were became irregular before intragastric administration, which exhibited similar oestrus cycles in the experimental and control groups after medication. At month 4 after intragastric administration, the ovaries of rats in the experimental group comprised atretic follicle, primordial follicle, developed follicles, as well as atretic follicles and corpus luteum, and the number was greater than that of the control group (P < 0.05), however, the coefficients of ovarian and uterine and increased body mass had no significant difference between the experimental and control groups. The results demonstrated that quercetin may promote ovarian follicular development and maturity, but no effect on the ovarian life span.

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