中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (15): 2675-2679.doi: 10.3969/j.issn.1673-8225.2010.15.004

• 骨组织构建 bone tissue construction • 上一篇    下一篇

去卵巢骨质疏松大鼠成骨细胞G1期调节蛋白的改变

吴银生1，林燕萍1，卢天祥2，林  煜1，黄云梅3，黄美雅3   

  1. 1福建中医学院中西医结合研究院骨病研究所，福建省福州市 350108；2福建省泉州市第一医院骨科，福建省泉州市  362000；3福建中医学院中西医结合研究院基础重点实验室，福建省福州市 350108
  • 出版日期:2010-04-09 发布日期:2010-04-09
  • 通讯作者: 林燕萍，福建中医学院中西医结合研究院骨病研究所，福建省福州市 350108 Lyp66@126.com
  • 作者简介:吴银生★，男，1978年生，福建省漳州市人，汉族，2008年福建中医学院毕业，博士，助理研究员，主要从事中西医结合骨病研究。 Wings197810@sina.com
  • 基金资助:

    课题为系列研究，分别获得国家自然科学基金项目(30572402)；福建省科技厅社发处重点项目(2009Y0028)；福建省科技厅青年人才项目(2008F3054)；福建省中西医结合老年性疾病重点实验室(福建中医学院)开放课题·陈可冀中西医结合发展基金资助项目(2008J1004-30、CKJ2008067)的资助。

Changes in G1 phase regulatory protein of osteoblasts in ovariectomized osteoporosis rats 

Wu Yin-sheng1, Lin Yan-ping1, Lu Tian-xiang2, Lin Yu1, Huang Yun-mei3, Huang Mei-ya3   

  1. 1 Institute of Bone Disease, Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou  350108, Fujian Province, China; 2 Department of Orthopaedics, Quanzhou First Hospital, Quanzhou  362000, Fujian Province, China; 3 Key Laboratory of Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou  350108, Fujian Province, China
  • Online:2010-04-09 Published:2010-04-09
  • Contact: Lin Yan-ping, Institute of Bone Disease, Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, Fujian Province, China Lyp66@126.com
  • About author:Wu Yin-sheng☆, Doctor, Assistant researcher, Institute of Bone Disease, Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, Fujian Province, China Wings197810@sina.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30572402*; Key Program of Social Development Department of Fujian Provincial Department of Science and Technology, No. 2009Y0028*; Youth Talent Program of Science and Technology Department of Fujian, No. 2008F3054*; Open Subjects of Fujian Provincial Key Laboratory of Integrative Medicine for Senile Diseases, (Fujian University of Traditional Chinese Medicine) · Chen Ke-ji Foundation for the Development of Integrative Medicine, No. 2008J1004-30, CKJ2008067*

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摘要：

背景：G1期调节蛋白对调控增殖细胞的增殖周期具有重要作用，目前关于成骨细胞G1期调节蛋白的研究甚少，绝经后骨质疏松与成骨细胞G1期调节蛋白的关系更未被阐明。
目的：观察去卵巢大鼠骨质疏松发病过程中成骨细胞G1期调节蛋白的改变，探讨绝经后骨质疏松症的发病机制。
方法：100只6月龄SD雌性大鼠，随机分成假手术组和模型组各50只，模型组进行双侧卵巢结扎切除术，假手术组除未行卵巢结扎切除外，其余步骤与手术组相同。所有大鼠于术后4，8，12，18，24周分批取材，每批每组各处死10只，取大鼠腰椎。运用免疫组织化学方法检测骨组织中成骨细胞G1期调节蛋白Cyclin D1、CDK4、p21蛋白表达。
结果与结论：Cyclin D1、CDK4蛋白阳性表达主要定位于骨小梁表面的成骨细胞。假手术组有Cyclin D1、CDK4蛋白阳性表达，模型组表达强于假手术组。p21蛋白阳性表达部位与Cyclin D1相似，假手术组和模型组均有明显阳性表达，但模型组的表达维持在较高水平，在各时期均明显高于同期假手术组(P < 0.01)。结果证实，去卵巢骨质疏松模型大鼠发病过程中，成骨细胞Cyclin D1、CDK4、p21蛋白出现明显高表达。提示成骨细胞增殖加快，同时成骨细胞周期受阻滞亦增多，成骨细胞数量相对不足，骨形成低于骨吸收，导致骨质疏松的发生。

关键词: 绝经后骨质疏松症, 细胞周期蛋白D1, 细胞周期蛋白质依赖激酶, 癌基因蛋白质p21, 骨组织工程

Abstract:

BACKGROUND: G1 phase regulatory protein plays an important role in regulating cell reproductive cycle. However, there are few reports concerning research of G1 phase regulatory protein of osteoblasts, and the relationship between postmenopausal osteoporosis and the G1 phase regulatory protein of osteoblasts have not been explained.
OBJECTIVE: To observe the change of G1 phase regulatory protein of osteoblasts in the ovariectomized osteoporosis rats, and to investigate the pathogenesis of postmenopausal osteoporosis.
METHODS: Totally 100 6-month-old SD female rats were randomly divided into sham surgery and model groups, with 50 animals in each group. Rats in the model group were performed bilaterally ovariectomy, those in the sham surgery group were subjected to sham surgery. Ten rats of each group were sacrificed at 4,8,12,18 and 24 weeks after operation, respectively. The expression of Cyclin D1, CDK4, and p21 protein in osteoblasts of lumbar vertebra were measured by immunohistochemistry.
RESULTS AND CONCLUSION: Cyclin D1 and CDK4-positive expression mainly located at osteoblasts which in the superficies of bone trabecula. There was Cyclin D1 and CDK4 positive expression in the sham surgery group, but was obviously smaller than that of the model group. The location of p21 positive expression was similar to Cyclin D1. Obviously positive expression of p21 appeared in the bone tissue of both groups, in which that of the model group maintained at a high level, and higher than that of the sham surgery group at different periods after operation (P < 0.01). The results demonstrated that Cyclin D1, CDK4 and p21 in osteoblasts were hyper-expressed in the process of osteoporosis in ovariectomized rats. It suggested that faster osteoblast proliferation and inhabited osteoblast cell cycles result in relative insufficiency of osteoblast, thus the bone formation is smaller than bone resorption, eventually, lead to the occurrence of osteoporosis.

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