中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (15): 2671-2674.doi: 10.3969/j.issn.1673-8225.2010.15.003

• 骨组织构建 bone tissue construction • 上一篇    下一篇

重组人骨形态发生蛋白2在下颌骨牵引成骨过程中的作用

田璐鸣，王稚英   

  1. 辽宁医学院附属口腔医院，辽宁省锦州市  121000
  • 出版日期:2010-04-09 发布日期:2010-04-09
  • 作者简介:田璐鸣★，男，1976年生，辽宁省彰武县人，汉族，2007年辽宁医学院口腔医学专业毕业，硕士，助理研究员，主治医师，主要从事颌骨牵引成骨方面的实验研究。 memopoor@163.com

Effects of recombinant human bone morphogenetic protein 2 on mandibular distraction 

Tian Lu-ming, Wang Zhi-ying   

  1. Affiliated Stomatologial Hospital of Liaoning Medical University, Jinzhou  121000, Liaoning Province, China
  • Online:2010-04-09 Published:2010-04-09
  • About author:Tian Lu-ming★, Master, Assistant researcher, Attending physician, Affiliated Stomatologial Hospital of Liaoning Medical University, Jinzhou 121000, Liaoning Province, China memopoor@163.com

PDF

366

被引次数

5

摘要：

背景：重组人骨形态发生蛋白2能诱导未分化的间充质细胞不可逆地分化形成软骨和骨，从而导致新骨的形成。
目的：观察在下颌骨牵引成骨过程中应用基因重组人骨形态发生蛋白2(rhBMP-2)对成骨的影响。
方法：选用日本成年大耳白兔45只，随机分为3组，建立下颌骨牵引模型，分别将空白胶原、1.5 mg和3.0 mg重组人骨形态发生蛋白2胶原复合物植入下颌骨切开处，固定、牵引0.4 mm/次，2次/d，共计5 d，总延长下颌骨4 mm。在稳定期第1，3，7，14，28天分别处死各组动物，取牵引区新生骨痂行放射学及组织学检测。
结果与结论：重组人骨形态发生蛋白2组中的新生骨组织较空白胶原对照组中多且成熟，3.0 mg重组人骨形态发生蛋白2组较1.5 mg重组人骨形态发生蛋白2组新骨形成多且成熟。结果表明，重组人骨形态发生蛋白2能促进兔下颌骨牵引成骨，并且具有浓度依赖性。

关键词: 下颌骨, 牵引成骨, 基因重组人骨形态发生蛋白2, 骨组织工程

Abstract:

BACKGROUND: Recombinant human bone morphogenetic protein 2 (rhBMP-2) can induce undifferentiated mesenchymal cells irreversibly differentiated into cartilage and bone, thus, lead to the formation of new bone.
OBJECTIVE: To study the effect of rhBMP-2 on the mandibular distraction.
METHODS: A total of 45 Japanese white rabbits were prepared for mandibular distraction models and randomly assigned into control, 1.5 mg rhBMP-2 and 3.0 mg rhBMP-2 groups. Collagen sponge and collagen binding proteins were implanted into mandibles of rabbits, respectively, and distraction 0.4 mm per time, twice per day for 5 days. The mandibles were lengthened    4 mm. All rats were sacrificed at 1, 3, 7, 14 and 28 days after operation, and the new generated bone were detected by X-ray and histological examination. 
RESULTS AND CONCLUSION: Compared with the control group, there were more neonatal bone tissues with maturity in the rhBMP-2 group, especially in the rhBMP-2 3.0 mg group. The results demonstrated that RhBMP-2 appears to be able to accelerate bone formation of mandibular distraction osteogenesis in a concentration-dependent manner. 

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