中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (13): 2399-2402.doi: 10.3969/j.issn.1673-8225.2010.13.030

• 骨与关节损伤基础实验 basic experiments of bone and joint injury • 上一篇    下一篇

Th1/Th2免疫应答系统在冠状动脉支架内再狭窄患者中的表达

李今朝1，张  军2   

  1. 1辽宁医学院附属第一医院心内科，辽宁省锦州市  121000；2辽宁医学院附属第二医院口腔内科，辽宁省锦州市  121000
  • 出版日期:2010-03-26 发布日期:2010-03-26
  • 通讯作者: 张 军，博士，辽宁医学院附属第二医院口腔内科，辽宁省锦州市 121000 cncnlady@126.com
  • 作者简介:李今朝☆，男，1969年生，辽宁省锦州市人，汉族，中国医科大学毕业，博士，副主任医师，主要从事冠心病介入治疗的研究。 medicaldoctor33@sina.com
  • 基金资助:

    辽宁省教育厅资助项目(30556013)。

Th1/Th2 immunological response expression in a patient of coronary arterial stent restenosis

Lin Jin-zhao1, Zhang Jun2   

  1. 1 Department of Cardiology, First Hospital Affiliated to Liaoning Medical University, Jinzhou 121000, Liaoning Province, China; 2 Department of Oral Medicine, Second Hospital Affiliated to Liaoning Medical University, Jinzhou   121000, Liaoning Province, China
  • Online:2010-03-26 Published:2010-03-26
  • Contact: Zhang Jun, Doctor, Department of Oral Medicine, Second Hospital Affiliated to Liaoning Medical University, Jinzhou 121000, Liaoning Province, China cncnlady@126.com
  • About author:Li Jin-zhao☆, Doctor, Associate chief physician, Department of Cardiology, First Hospital Affiliated to Liaoning Medical University, Jinzhou 121000, Liaoning Province, China medicaldoctor33@sina.com
  • Supported by:

    the Foundation of Education Department of Liaoning Province, No. 30556013*

PDF

358

被引次数

6

摘要：

背景：支架再狭窄的机制尚未阐明，炎症失衡在多种疾病中起重要做用，可能与支架再狭窄有关。
目的：观察冠状动脉支架置入后支架内再狭窄患者辅助T细胞亚型Th1样细胞因子γ-干扰素、白细胞介素2和Th2样细胞因子白细胞介素4、白细胞介素10在血清中的分布特点。
方法：应用酶联免疫吸附法测定32例冠状动脉内支架置入后合并支架内再狭窄患者血清标本中γ-干扰素、白细胞介素2、白细胞介素4、白细胞介素10的浓度(实验2组)，并与未行支架置入但排除冠心病患者30例(对照1组)、未行支架置入的冠心病患者30例(对照2组)及行支架置入但是无支架内狭窄患者30例(实验1组)进行比较。
结果与结论：对照2组血清中γ-干扰素、白细胞介素2、白细胞介素4、白细胞介素10的水平与对照1组相比，差异无显著性意义(P > 0.05)；实验1组各指标与对照1组相比，差异亦无显著性意义(P > 0.05)；实验2组患者血清中γ-干扰素、白细胞介素2水平高于其他3组(P < 0.01)；白细胞介素4、白细胞介素10水平则低于对照1组和实验1组(P < 0.01)。结果表明支架内再狭窄患者存在Th1、Th2炎症水平的失衡，Th1型细胞因子上调，Th2型细胞因子下调，提示Th1/Th2失衡可能是冠状动脉支架内再狭窄的重要原因。

关键词: 支架, 冠心病, T淋巴细胞, 辅助诱导, 干扰素Ⅱ型, 白细胞介素

Abstract:

BACKGROUND: Mechanism of restenosis remains unclear. Inflammatory unbalance plays an important role in various diseases, being related to stent restenosis.
OBJECTIVE: To evaluate the distribution characteristics of Th1-type cytokines [interferon-γ (INF-r) and interleukin-2 (IL-2)] and Th2-type cytokines (IL-4 and IL-10) in the serum of the patients with the in-stent restenosis following coronary artery stent implantation.
METHODS: The concentrations of INF-γ, IL-2, IL-4, and IL-10 in the serum of 32 patients with in-stent restenosis following coronary artery implantation (experimental 2 group) were measured with enzyme linked immuno-sorbent assay (ELISA). The serum levels of the four cytokines were compared with the subjects without stent implantation and coronary artery disease (n=30, control I group), the patients without stent implantation but with the coronary artery disease (n=30, control II group), and the patients with stent implantation but without in-stent restenosis (n=30, experimental I group).
RESULTS AND CONCLUSION: There was no significant difference in the level of the INF-γ, IL-2, IL-4, and IL-10 between control 2 group and control 1 group (P > 0.05); while there was also no significant difference in those levels between experimental 1 group and control 1 group (P > 0.05). The levels of INF-γ and IL-2 in the experimental 2 group were significantly higher than other three groups (P < 0.01); but the levels of IL-4 and IL-10 in the experimental 2 group were significantly lower than control 1 and experimental 1 groups (P < 0.01). The results demonstrated that Th1 and Th2 inflammatory unbalances, i.e., up-regulation of Th1 and down-regulation of Th2, occurred in patients with in-stent restenosis, which might be the key factors for in-stent restenosis following coronary artery implantation.

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