中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (12): 2137-2140.doi: 10.3969/j.issn.1673-8225.2010.12.012

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

 一种新型可切削生物活性微晶玻璃的生物安全性评价

郝玉全1，高莹娇2，秦小梅3，韩雪松4，刘敏达5，艾红军1   

  1. 中国医科大学口腔医学院，1口腔修复科， 2口腔正畸科，辽宁省沈阳市  110002；3东北大学材料与冶金学院，辽宁省沈阳市  110004；  4武警辽宁省总队医院口腔科，辽宁省沈阳市  110034；5中国医科大学口腔医学院，辽宁省沈阳市  110002
  • 出版日期:2010-03-19 发布日期:2010-03-19
  • 通讯作者: 艾红军，教授，主任医师，博士生导师，中国医科大学口腔医学院口腔修复科，辽宁省沈阳市 110002 aih0620@yahoo.com.cn
  • 作者简介:郝玉全★，男，1975年生，辽宁省沈阳市人，汉族，2004年中国医科大学毕业，硕士，讲师，主治医师，主要从事生物医用材料的研究。 Yqhao056@163.com

Biological safety assessment of a new machinable bioactive glass-ceramic

Hao Yu-quan1, Gao Ying-jiao2, Qin Xiao-mei3, Han Xue-song4, Liu Min-da5, Ai Hong-jun1   

  1. 1 Department of Prosthodontics, School of Stomatology, China Medical University, Shenyang  110002, Liaoning Province, China; 2 Department of Orthodontics, School of Stomatology, China Medical University, Shenyang  110002, Liaoning Province, China; 3 College of Materials and Metallurgy, Northeastern University, Shenyang 110004, Liaoning Province, China; 4 Department of Stomatology, Liaoning Provincial Corps Hospital of Chinese People’s Armed Police Forces, Shenyang  110034, Liaoning Province, China; 5 School of Stomatology, China Medical University, Shenyang  110002, Liaoning Province, China
  • Online:2010-03-19 Published:2010-03-19
  • Contact: Ai Hong-jun, Professor, Chief physician, Doctoral supervisor, Department of Prosthodontics, School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China aih0620@yahoo.com.cn
  • About author:Hao Yu-quan★, Master, Lecturer, Attending physician, Department of Prosthodontics, School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China yqhao056@163.com

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摘要：

背景：自主设计的可切削生物活性微晶玻璃，在保证抗弯强度的同时，有效降低了熔制温度、制备成本。在临床应用前，除必要的机械、理化性能研究外，还需进行生物相容性评估。
目的：观察新型可切削生物活性微晶玻璃对小鼠有无全身毒性作用，评价其生物安全性。
方法：将纯系昆明种小鼠随机分为实验组、阴性对照组和阳性对照组，实验组经口灌注可切削生物活性微晶玻璃的阿拉伯胶混悬液，阴性对照组灌注等体积阿拉伯胶，阳性对照组灌注醋酸铅溶液,于灌注后24，48，72 h观察小鼠一般状态、毒性表现并记录体质量变化。最后处死小鼠取出肝、肾，做光、电镜检查。
结果与结论：72 h内，实验组小鼠体质量未下降，与阴性对照组比较，差异无显著性意义(P > 0.05)，肝、肾组织未见病理改变。提示该新型可切削生物活性微晶玻璃经短期口服后对机体无全身毒性。

关键词: 微晶玻璃, 生物安全性, 急性全身毒性实验, 小鼠, 生物材料

Abstract:

BACKGROUND: The machinable bioactive glass-ceramic we designed recently have a lower melting temperature, and preparation of cost while bending strength in ensuring the same time. Prior to clinical application, biocompatibility should be evaluated.
OBJECTIVE: To observe the new machinable bioactive glass-ceramics on the availability of systemic toxicity in mice and evaluate its biological security.
METHODS: The Kunming mice were randomly divided into an experimental group, negative control group and positive control group. Mice in experimental group were orally perfused with the suspension of machinable bioactive glass-ceramics and Arabic gum. While the mice in the negative control group were treated with an equal volume of Arabic gum, and the mice in the positive control group were treated with an equal volume of lead acetate solution. The general state and toxicity of mice were observed and body mass was recorded after 24 hours, 48 hours, and 72 hours. Finally the mice were sacrificed, and the morphological changes in liver and kidney were then examined by light and electron microscopy.
RESULTS AND CONCLUSION: The body mass of mice in the experimental group was not decreased significantly compared with the mice in the negative control group in 72 hours (P > 0.05). Pathological changes of liver and kidney in the experimental group did not be found. The new machinable bioactive glass-ceramic did not show systemic toxicity for a short term.

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