中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (3): 473-476.doi: 10.3969/j.issn.1673-8225.2010.03.022

• 生物材料基础实验 basic experiments of biomaterials • 上一篇    下一篇

硬性透气性角膜接触镜材料体外溶菌酶的短期吸附动力学

章  瑾1，万子义2，南开辉3，郑  琪1，瞿  佳4，奚廷斐2，5   

  1. 1温州医学院检验医学院，浙江省温州市 325035；   2温州医学院生物材料与组织工程研究所，浙江省温州市 325035；   3温州市生物材料工程技术研究中心，浙江省温州市 325027；4温州医学院眼视光学院，浙江省温州市 325000；5北京大学前沿交叉学科研究院，北京市 100871
  • 出版日期:2010-01-15 发布日期:2010-01-15
  • 通讯作者: 瞿 佳，教授，温州医学院眼视光学院，浙江省温州市 325000 zxt-dr@wzzj.cn
  • 作者简介:章 瑾★，女，1983年生，河南省安阳市人，汉族，温州医学院在读硕士，主要从事医用生物材料研究。 zhang_jin_222@163.com

The kinetics of in vitro lysozyme deposition on rigid gas-permeable contact lens for a short period

Zhang Jin1, Wan Zi-yi2, Nan Kai-hui 3, Zheng Qi1, Qu Jia4, Xi Ting-fei 2,5   

  1. 1 School of Medical Laboratory Science, Wenzhou Medical College, Wenzhou 325035, Zhejiang Province, China; 2 Institute of Biomaterials & Tissue Engineering, Wenzhou Medical College, Wenzhou 325035, Zhejiang Province, China; 3 Wenzhou Engineering Research Center for Biomaterials, Wenzhou  325027, Zhejiang Province, China; 4 School of Ophthalmology & Optometry, Wenzhou Medical College, Wenzhou  325000, Zhejiang Province, China; 5 Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
  • Online:2010-01-15 Published:2010-01-15
  • Contact: Qu Jia, Professor, School of Ophthalmology & Optometry, Wenzhou Medical College, Wenzhou 325000, Zhejiang Province, China zxt-dr@wzzj.cn
  • About author:Zhang Jin★, Studying for master’s degree, School of Medical Laboratory Science, Wenzhou Medical College, Wenzhou 325035, Zhejiang Province, China zhang_jin_222@163.com

PDF

483

被引次数

2

摘要：

背景：镜片沉淀物的形成是角膜接触镜配戴的常见问题之一，其中蛋白沉淀最易形成。研究氟硅丙烯酸酯硬镜溶菌酶吸附动力学可进一步完善硬镜蛋白吸附数据，为降低其表面蛋白吸附量和预防硬镜表面污染提供有意义的指导。
目的：考察氟硅丙烯酸酯硬镜在体外对溶菌酶的吸附情况。
方法：配制质量浓度为2.0 g/L溶菌酶溶液(溶液Ⅰ)及不同浓度三氟乙酸溶液；解析率实验，将对照组与实验组镜片浸入溶液Ⅰ37 ℃振荡孵育，Hank’s平衡盐溶液清洗氟硅丙烯酸酯硬镜，然后将对照组镜片浸入去离子水中，实验组镜片浸入不同浓度三氟乙酸，于不同时间点取出；溶菌酶短期吸附动力学实验，对照组镜片浸入去离子水中，实验组镜片浸入溶液Ⅰ37 ℃振荡孵育不同时间点，Hank’s平衡盐溶液清洗氟硅丙烯酸酯硬镜，后用0.2%三氟乙酸解析吸附溶菌酶；BCA法测定各溶液中溶菌酶的量。
结果与结论：氟硅丙烯酸酯硬镜吸附溶菌酶可用三氟乙酸解析，三氟乙酸解析溶菌酶受解析时间和解析浓度的影响。三氟乙酸解析溶菌酶最佳时间1 h，最适浓度0.2%。氟硅丙烯酸酯硬镜吸附溶菌酶(体外模拟24 h)，10 min~1 h溶菌酶吸附量递增，1 h达吸附饱和，1~24 h吸附量稳定，饱和吸附量为0.349 mg/cm2。解析时间为10，30 min时，溶菌酶解析率较低，40 min~24 h解析率较好(90%~100%)。

关键词: 氟硅丙烯酸酯硬镜, 溶菌酶, 短期, 吸附动力学, 解析率

Abstract:

BACKGROUND: The contact lenses were easily contaminated by adsorbing components from the tear film, particularly protein after wearing for a period of time. Lysozyme adsorption dynamics of ?uorosilicone acrylate contact lenses has been studied in order to further improve data of protein adsorption, reduce adsorbing amount of surface protein, and prevent surface contamination of contact lenses.
OBJECTIVE: To investigate the adsorption dynamics of ?uorosilicone acrylate contact lenses to lysozyme in vitro.
METHODS: A stock solution of lysozyme was prepared in Hanks balanced salt solution (2.0 g/L, solution I) and different trifluoroacetic acid (TFA) concentrations were prepared. Recovery experiment, the contact lenses were placed in shaking incubator at 37 ℃ for varying time intervals. After incubation there was a single rinsing in Hanks balanced salt solution. Contact lenses in control group were placed in diluted water, and contact lenses in the other group were placed in different concentrations of TFA. For deposition, FSA contact lenses in experimental group were placed in shaking incubator at 37 ℃ for varying time intervals. After incubation there was a single rinsing in Hanks balanced salt solution. Then FSA contact lenses were immersed in 0.2% TFA solution. The amount of lysozyme was assayed with BCA method.
RESULTS AND CONCLUSION: Lysozyme which attached to ?uorosilicone acrylate contact lenses could be resolved by TFA, and the recovery was influenced by the immersed time and the concentration of TFA. The optimal time was 1 hour, and the optimum concentration was 0.2%. The adsorption dynamics of lysozyme on FSA contact lenses was a second-phased process, i.e., lysozyme adsorption increased rapidly during 10 minutes-1 hour, reached a plateau at 1 hour, stably adsorbed during 1-24 hours, and reached a saturation of 0.349 mg/cm2. The recovery of lysozyme was lower at 10 and 30 minutes, but reached 90%-100% while the time of incubation was between 40 minutes and 24 hours.

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