中国组织工程研究

中国组织工程研究 ›› 2026, Vol. 30 ›› Issue (32): 8345-8351.doi: 10.12307/2026.885

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

新型口腔修复材料聚醚醚酮促进牙髓干细胞增殖的机制

吴  琳，陆  统   

  1. 浙江特殊教育职业学院，浙江省杭州市  310023
  • 接受日期:2026-02-09 出版日期:2026-11-18 发布日期:2026-04-23
  • 通讯作者: 陆统，副教授，浙江特殊教育职业学院，浙江省杭州市 310023
  • 作者简介:吴琳，女，1986年生，湖北省十堰市人，汉族，硕士，主治医师，副教授，主要从事基础医学/口腔医学方面的研究。
  • 基金资助:
    2025年浙江省教育科学规划重点课题“教育数字化转型背景下高职院校教师数字素养测评与提升策略研究”(2025SB144)，项目负责人：吴琳；2025年度浙江省教育厅一般科研项目(Y202559497)：基于AI的基层慢病智能筛查模型构建研究，项目负责人：吴琳；浙江省教育厅2023年度高校国内访问工程师“校企合作项目”(FG2023368)，项目负责人：吴琳

Mechanism by which polyetheretherketone, a novel oral restorative material, promotes the proliferation of dental pulp stem cells

Wu Lin, Lu Tong   

  1. Zhejiang Vocational College of Special Education, Hangzhou 310023, Zhejiang Province, China
  • Accepted:2026-02-09 Online:2026-11-18 Published:2026-04-23
  • Contact: Lu Tong, Associate professor, Zhejiang Vocational College of Special Education, Hangzhou 310023, Zhejiang Province, China
  • About author:Wu Lin, MS, Attending physician, Associate professor, Zhejiang Vocational College of Special Education, Hangzhou 310023, Zhejiang Province, China
  • Supported by:
    Key Project of Zhejiang Provincial Educational Science Planning in 2025, No. 2025SB144: Research on the Evaluation and Improvement Strategies of Digital Literacy of Teachers in Higher Vocational Colleges under the Background of Educational Scientific Transformation (to WL); General Scientific Research Project of Zhejiang Provincial Department of Education in 2025, No. Y202559497: Research on the Construction of AI-Based Intelligent Screening Model for Chronic Diseases at the Grassroots Level (to WL); University Domestic Visiting Engineer "School-Enterprise Cooperation Project" of Zhejiang Provincial Department of Education in 2023, No. FG2023368 (to WL) 

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摘要：

文题释义：
聚醚醚酮：是一种高性能的热塑性工程塑料，分子主链由重复的醚键和酮键构成。聚醚醚酮具有优异的耐高温、耐腐蚀、耐磨损和电绝缘性能，广泛应用于航空航天、医疗器械和汽车等领域。
牙髓干细胞：是源自牙齿内部牙髓组织的成体干细胞，具有多向分化潜能和神经嵴外胚层起源特征，在牙齿再生、糖尿病治疗及神经系统修复等领域展现出临床应用潜力。

背景：聚醚醚酮作为新型盖髓材料用于活髓保存治疗时，其表面特性与释放的活性成分可协同调控牙髓干细胞增殖，但具体的作用机制尚未明确。
目的：探究聚醚醚酮对牙髓干细胞增殖的影响及相关机制。
方法：①分离培养人牙髓干细胞。采用不同质量浓度[0(对照)，0.1，1，10 mg/mL]聚醚醚酮培养人牙髓干细胞48 h，EdU染色与CCK-8法检测细胞增殖，免疫印迹检测细胞外信号调节激酶、磷酸化细胞外信号调节激酶蛋白表达。采用1 mg/mL聚醚醚酮培养人牙髓干细胞0，24，48，72，96，120 h，CCK-8法检测细胞增殖。采用0(对照)，1 mg/mL聚醚醚酮培养人牙髓干细胞48 h，流式细胞仪检测细胞周期。②将人牙髓干细胞分3组培养：对照组不进行任何干预，聚醚醚酮组加入1 mg/mL聚醚醚酮，聚醚醚酮+U0126组加入1 mg/mL聚醚醚酮与10 µmol/L细胞外信号调节激酶抑制剂U0126，培养48 h后，EdU染色与CCK-8法检测细胞增殖，流式细胞仪检测细胞周期，免疫印迹检测细胞外信号调节激酶、磷酸化细胞外信号调节激酶蛋白表达。
结果与结论：①1 mg/mL聚醚醚酮组细胞增殖快于其余3组，磷酸化细胞外信号调节激酶蛋白表达高于对照组、0.1 mg/mL聚醚醚酮组，因此，后续实验选择1 mg/mL聚醚醚酮进行干预。1 mg/mL聚醚醚酮培养96 h的细胞增殖快于24，48，72 h。1 mg/mL聚醚醚酮组G0/G1期细胞比例低于对照组，S期细胞比例高于对照组。②聚醚醚酮组细胞增殖快于对照组、聚醚醚酮+U0126组，G0/G1期细胞比例低于对照组、聚醚醚酮+U0126组，S期细胞比例与磷酸化细胞外信号调节激酶蛋白表达高于对照组、聚醚醚酮+U0126组。③结果表明，聚醚醚酮通过激活丝裂原活化蛋白激酶/细胞外信号调节激酶促进牙髓干细胞增殖。
https://orcid.org/0009-0003-2174-2599(吴琳)

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料；口腔生物材料；纳米材料；缓释材料；材料相容性；组织工程

关键词: 新型口腔修复材料">, 聚醚醚酮">, MAPK/ERK信号通路">, 牙髓干细胞">, 细胞增殖">, 细胞周期

Abstract: BACKGROUND: Polyetheretherketone, as a novel pulp capping material used in vital pulp therapy, can synergistically regulate the proliferation of dental pulp stem cells through its surface characteristics and released active components, but the specific mechanism remains unclear.
OBJECTIVE: To investigate the effect of polyetheretherketone on the proliferation of dental pulp stem cells and the related mechanisms.
METHODS: (1) Human dental pulp stem cells were isolated and cultured. Human dental pulp stem cells were cultured with different mass concentrations of polyetheretherketone [0 (control), 0.1, 1, 10 mg/mL] for 48 hours. Cell proliferation was detected by EdU staining and CCK-8 assay. The expression of extracellular signal-regulated kinase and phosphorylated extracellular signal-regulated kinase proteins was detected by western blot assay. Human dental pulp stem cells were cultured with 1 mg/mL polyetheretherketone for 0, 24, 48, 72, 96, and 120 hours. Cell proliferation was detected by CCK-8 assay. Human dental pulp stem cells were cultured with 0 (control) and 1 mg/mL polyetheretherketone for 48 hours. Cell cycle was detected by flow cytometry. (2) Human dental pulp stem cells were divided into three groups: control group (no intervention), polyetheretherketone group (1 mg/mL polyetheretherketone), and polyetheretherketone+U0126 group (1 mg/mL polyetheretherketone and 10 µmol/L extracellular signal-regulated kinase inhibitor U0126). After 48 hours of culture, cell proliferation was detected by EdU staining and CCK-8 assay. Cell cycle was detected by flow cytometry. The protein expression of extracellular signal-regulated kinase and phosphorylated extracellular signal-regulated kinase was detected by western blot assay. 
RESULTS AND CONCLUSION: (1) The cell proliferation in the 1 mg/mL polyetheretherketone group was faster than that in the other three groups, and the expression of phosphorylated extracellular signal-regulated kinase protein was higher than that in the control group and the 0.1 mg/mL polyetheretherketone group. Therefore, 1 mg/mL polyetheretherketone was selected for subsequent experiments. Cell proliferation after 96 hours of culture with 1 mg/mL polyetheretherketone was faster than that after 24, 48, and 72 hours. The proportion of cells in the G0/G1 phase in the 1 mg/mL polyetheretherketone group was lower than that in the control group, and the proportion of cells in the S phase was higher than that in the control group. (2) Cell proliferation in the polyetheretherketone group was faster than that in the control group and the polyetheretherketone+U0126 group. The proportion of cells in the G0/G1 phase was lower in the polyetheretherketone group than in the control group and the polyetheretherketone+U0126 group, while the proportion of cells in the S phase and the expression of phosphorylated extracellular signal-regulated kinase protein were higher in the polyetheretherketone group than in the control group and the polyetheretherketone+U0126 group. (3) The results indicate that polyetheretherketone promotes dental pulp stem cell proliferation by activating the mitogen-activated protein kinase/extracellular signal-regulated kinase signaling pathway.

Key words: novel oral restorative materials">, polyetheretherketone">, MAPK/ERK signaling pathway">, dental pulp stem cells">, cell proliferation">, cell cycle

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